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Expression of SANT/HTH Myb mRNA, a plant morphogenesis-regulating transcription factor, changes due to viroid infection
J. Matoušek, RJ. Piernikarczyk, A. Týcová, GS. Duraisamy, T. Kocábek, G. Steger,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- interakce hostitele a patogenu MeSH
- listy rostlin růst a vývoj metabolismus virologie MeSH
- malá nekódující RNA genetika metabolismus MeSH
- messenger RNA genetika metabolismus MeSH
- molekulární sekvence - údaje MeSH
- nemoci rostlin genetika virologie MeSH
- regulátory růstu rostlin genetika metabolismus MeSH
- RNA virová genetika metabolismus MeSH
- rostlinné proteiny genetika metabolismus MeSH
- sekvenční analýza RNA MeSH
- Solanum lycopersicum genetika metabolismus virologie MeSH
- tabák genetika metabolismus virologie MeSH
- transkripční faktory genetika metabolismus MeSH
- viroidy genetika patogenita fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Potato spindle tuber viroid (PSTVd) belongs to plant-pathogenic, circular, non-coding RNAs. Its propagation is accompanied by (mis)regulation of host genes and induction of pathogenesis symptoms including changes of leaf morphogenesis depending on the strength of viroid variant. We found strong genotype-dependent suppression of tomato morphogenesis-regulating transcription factor SANT/HTH-Myb (SlMyb) due to viroid pathogenesis. Its relative mRNA level was found to be significantly decreased in PSTVd-sensitive tomato (cvs Rutgers and Heinz 1706) due to degradation processes, but increased in PSTVd-tolerant (cv. Harzfeuer). In heterologous system of Nicotiana benthamiana, we observed a SlMyb-associated necrotic effect in agroinfiltrated leaf sectors during ectopic overexpression. Leaf sector necroses were accompanied by activation of nucleolytic enzymes but were suppressed by a strongly pathogenic PSTVd variant. Contrary to that, PSTVd's effect was inhibited by the silencing suppressor p19. It was found that in both, Solanum lycopersicum leaves and N. benthamiana leaf sectors, SlMyb mRNA degradation was significantly stronger in viroid-infected tissues. Necroses induction as well as gene silencing experiments using the SANT/HTH-Myb homologues revealed involvement of this Myb in physiological changes like distortions in flower morphogenesis and growth suppression.
Citace poskytuje Crossref.org
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- $a Matoušek, Jaroslav $u BC ASCR v. v. i., Institute of Plant Molecular Biology, Branišovská 31, 37005 České Budějovice, Czech Republic. Electronic address: jmat@umbr.cas.cz.
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- $a Potato spindle tuber viroid (PSTVd) belongs to plant-pathogenic, circular, non-coding RNAs. Its propagation is accompanied by (mis)regulation of host genes and induction of pathogenesis symptoms including changes of leaf morphogenesis depending on the strength of viroid variant. We found strong genotype-dependent suppression of tomato morphogenesis-regulating transcription factor SANT/HTH-Myb (SlMyb) due to viroid pathogenesis. Its relative mRNA level was found to be significantly decreased in PSTVd-sensitive tomato (cvs Rutgers and Heinz 1706) due to degradation processes, but increased in PSTVd-tolerant (cv. Harzfeuer). In heterologous system of Nicotiana benthamiana, we observed a SlMyb-associated necrotic effect in agroinfiltrated leaf sectors during ectopic overexpression. Leaf sector necroses were accompanied by activation of nucleolytic enzymes but were suppressed by a strongly pathogenic PSTVd variant. Contrary to that, PSTVd's effect was inhibited by the silencing suppressor p19. It was found that in both, Solanum lycopersicum leaves and N. benthamiana leaf sectors, SlMyb mRNA degradation was significantly stronger in viroid-infected tissues. Necroses induction as well as gene silencing experiments using the SANT/HTH-Myb homologues revealed involvement of this Myb in physiological changes like distortions in flower morphogenesis and growth suppression.
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