-
Je něco špatně v tomto záznamu ?
Chiral recognition of bilirubin and biliverdin in liposomes and micelles
P. Novotná, F. Králík, M. Urbanová,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- biliverdin chemie metabolismus MeSH
- cholesterol analogy a deriváty metabolismus MeSH
- kompetitivní vazba MeSH
- kyseliny cholové metabolismus MeSH
- lidé MeSH
- micely * MeSH
- molekulární konformace MeSH
- molekulární modely MeSH
- sérový albumin hovězí metabolismus MeSH
- skot MeSH
- stereoizomerie MeSH
- unilamelární lipozómy chemie metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The structural formula of biologically important chiral pigments bilirubin and biliverdin differs only by one double bond. We showed that this results in dissimilar interactions with two models of membranes: cationic liposomes composed of 3β-[N-(N',N'-dimethylaminoethane)-carbamoyl]cholesterol and zwitterionic micelles from 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS). While the liposomes recognized the P-form of bilirubin, the micelles recognized its M-form. Both recognized the P-form of biliverdin. Our study also comprised ternary systems consisting of the pigments, model membranes and serum albumin (human and bovine). Bilirubin preferentially interacted with the albumins even in the presence of the liposomes. On the other hand, biliverdin preferred the liposomes. Remarkably, the presence of CHAPS completely changed the biliverdin binding to the protein. Because our study was oriented on different chiral interactions, a chiroptical method of electronic circular dichroism was chosen as the principal method to study our systems. As complementary methods, UV-vis absorption and fluorescence emission were used.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16020678
- 003
- CZ-PrNML
- 005
- 20160726092507.0
- 007
- ta
- 008
- 160722s2015 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.bpc.2015.06.001 $2 doi
- 024 7_
- $a 10.1016/j.bpc.2015.06.001 $2 doi
- 035 __
- $a (PubMed)26071845
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Novotná, Pavlína $u Department of Analytical Chemistry, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic.
- 245 10
- $a Chiral recognition of bilirubin and biliverdin in liposomes and micelles / $c P. Novotná, F. Králík, M. Urbanová,
- 520 9_
- $a The structural formula of biologically important chiral pigments bilirubin and biliverdin differs only by one double bond. We showed that this results in dissimilar interactions with two models of membranes: cationic liposomes composed of 3β-[N-(N',N'-dimethylaminoethane)-carbamoyl]cholesterol and zwitterionic micelles from 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS). While the liposomes recognized the P-form of bilirubin, the micelles recognized its M-form. Both recognized the P-form of biliverdin. Our study also comprised ternary systems consisting of the pigments, model membranes and serum albumin (human and bovine). Bilirubin preferentially interacted with the albumins even in the presence of the liposomes. On the other hand, biliverdin preferred the liposomes. Remarkably, the presence of CHAPS completely changed the biliverdin binding to the protein. Because our study was oriented on different chiral interactions, a chiroptical method of electronic circular dichroism was chosen as the principal method to study our systems. As complementary methods, UV-vis absorption and fluorescence emission were used.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a biliverdin $x chemie $x metabolismus $7 D001664
- 650 _2
- $a kompetitivní vazba $7 D001667
- 650 _2
- $a skot $7 D002417
- 650 _2
- $a cholesterol $x analogy a deriváty $x metabolismus $7 D002784
- 650 _2
- $a kyseliny cholové $x metabolismus $7 D002793
- 650 _2
- $a lidé $7 D006801
- 650 12
- $a micely $7 D008823
- 650 _2
- $a molekulární modely $7 D008958
- 650 _2
- $a molekulární konformace $7 D008968
- 650 _2
- $a sérový albumin hovězí $x metabolismus $7 D012710
- 650 _2
- $a stereoizomerie $7 D013237
- 650 _2
- $a unilamelární lipozómy $x chemie $x metabolismus $7 D053835
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Králík, František $u Department of Analytical Chemistry, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic.
- 700 1_
- $a Urbanová, Marie $u Department of Physics and Measurements, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic. Electronic address: Marie.Urbanova@vscht.cz.
- 773 0_
- $w MED00000773 $t Biophysical chemistry $x 1873-4200 $g Roč. 205, č. - (2015), s. 41-50
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/26071845 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20160722 $b ABA008
- 991 __
- $a 20160726092727 $b ABA008
- 999 __
- $a ok $b bmc $g 1155348 $s 945206
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 205 $c - $d 41-50 $e 20150604 $i 1873-4200 $m Biophysical chemistry $n Biophys Chem $x MED00000773
- LZP __
- $a Pubmed-20160722
