Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Cellular Responses Modulated by FGF-2 Adsorbed on Albumin/Heparin Layer-by-Layer Assemblies

M. Kumorek, D. Kubies, E. Filová, M. Houska, N. Kasoju, E. Mázl Chánová, R. Matějka, M. Krýslová, L. Bačáková, F. Rypáček,

. 2015 ; 10 (5) : e0125484. [pub] 20150506

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc16020825

In a typical cell culture system, growth factors immobilized on the cell culture surfaces can serve as a reservoir of bio-signaling molecules, without the need to supplement them additionally into the culture medium. In this paper, we report on the fabrication of albumin/heparin (Alb/Hep) assemblies for controlled binding of basic fibroblast growth factor (FGF-2). The surfaces were constructed by layer-by-layer adsorption of polyelectrolytes albumin and heparin and were subsequently stabilized by covalent crosslinking with glutaraldehyde. An analysis of the surface morphology by atomic force microscopy showed that two Alb/Hep bilayers are required to cover the surface of substrate. The formation of the Alb/Hep assemblies was monitored by the surface plasmon resonance (SPR), the infrared multiinternal reflection spectroscopy (FTIR MIRS) and UV/VIS spectroscopy. The adsorption of FGF-2 on the cross-linked Alb/Hep was followed by SPR. The results revealed that FGF-2 binds to the Alb/Hep assembly in a dose and time-dependent manner up to the surface concentration of 120 ng/cm(2). The bioactivity of the adsorbed FGF-2 was assessed in experiments in vitro, using calf pulmonary arterial endothelial cells (CPAE). CPAE cells could attach and proliferate on Alb/Hep surfaces. The adsorbed FGF-2 was bioactive and stimulated both the proliferation and the differentiation of CPAE cells. The improvement was more pronounced at a lower FGF-2 surface concentration (30 ng/cm(2)) than on surfaces with a higher concentration of FGF-2 (120 ng/cm(2)).

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc16020825
003      
CZ-PrNML
005      
20161216093538.0
007      
ta
008      
160722s2015 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1371/journal.pone.0125484 $2 doi
024    7_
$a 10.1371/journal.pone.0125484 $2 doi
035    __
$a (PubMed)25945799
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Kumorek, Marta $u Department of Biomaterials and Bioanalogous Systems, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic v.v.i., Prague, Czech Republic.
245    10
$a Cellular Responses Modulated by FGF-2 Adsorbed on Albumin/Heparin Layer-by-Layer Assemblies / $c M. Kumorek, D. Kubies, E. Filová, M. Houska, N. Kasoju, E. Mázl Chánová, R. Matějka, M. Krýslová, L. Bačáková, F. Rypáček,
520    9_
$a In a typical cell culture system, growth factors immobilized on the cell culture surfaces can serve as a reservoir of bio-signaling molecules, without the need to supplement them additionally into the culture medium. In this paper, we report on the fabrication of albumin/heparin (Alb/Hep) assemblies for controlled binding of basic fibroblast growth factor (FGF-2). The surfaces were constructed by layer-by-layer adsorption of polyelectrolytes albumin and heparin and were subsequently stabilized by covalent crosslinking with glutaraldehyde. An analysis of the surface morphology by atomic force microscopy showed that two Alb/Hep bilayers are required to cover the surface of substrate. The formation of the Alb/Hep assemblies was monitored by the surface plasmon resonance (SPR), the infrared multiinternal reflection spectroscopy (FTIR MIRS) and UV/VIS spectroscopy. The adsorption of FGF-2 on the cross-linked Alb/Hep was followed by SPR. The results revealed that FGF-2 binds to the Alb/Hep assembly in a dose and time-dependent manner up to the surface concentration of 120 ng/cm(2). The bioactivity of the adsorbed FGF-2 was assessed in experiments in vitro, using calf pulmonary arterial endothelial cells (CPAE). CPAE cells could attach and proliferate on Alb/Hep surfaces. The adsorbed FGF-2 was bioactive and stimulated both the proliferation and the differentiation of CPAE cells. The improvement was more pronounced at a lower FGF-2 surface concentration (30 ng/cm(2)) than on surfaces with a higher concentration of FGF-2 (120 ng/cm(2)).
650    _2
$a albuminy $x farmakologie $7 D000418
650    _2
$a zvířata $7 D000818
650    _2
$a skot $7 D002417
650    _2
$a buněčná adheze $x účinky léků $7 D002448
650    _2
$a buněčné kultury $x metody $7 D018929
650    _2
$a buněčná diferenciace $x účinky léků $7 D002454
650    _2
$a proliferace buněk $x účinky léků $7 D049109
650    _2
$a kultivační média $x chemie $7 D003470
650    _2
$a endoteliální buňky $x cytologie $7 D042783
650    _2
$a fibroblastový růstový faktor 2 $x metabolismus $x farmakologie $7 D016222
650    _2
$a heparin $x farmakologie $7 D006493
650    _2
$a lidé $7 D006801
650    _2
$a mikroskopie atomárních sil $7 D018625
650    _2
$a vazba proteinů $7 D011485
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Kubies, Dana $u Department of Biomaterials and Bioanalogous Systems, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic v.v.i., Prague, Czech Republic.
700    1_
$a Filová, Elena $u Department of Biomaterials and Tissue Engineering, Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., Prague, Czech Republic.
700    1_
$a Houska, Milan $u Department of Biomaterials and Bioanalogous Systems, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic v.v.i., Prague, Czech Republic.
700    1_
$a Kasoju, Naresh $u Department of Biomaterials and Bioanalogous Systems, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic v.v.i., Prague, Czech Republic.
700    1_
$a Mázl Chánová, Eliška $u Department of Biomaterials and Bioanalogous Systems, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic v.v.i., Prague, Czech Republic.
700    1_
$a Matějka, Roman $u Department of Biomaterials and Tissue Engineering, Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., Prague, Czech Republic; Department of Medical Biophysics and Informatics, Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic; Department of Biomedical Technology, Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic.
700    1_
$a Krýslová, Markéta $u Department of Biomaterials and Tissue Engineering, Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., Prague, Czech Republic. $7 xx0268307
700    1_
$a Bačáková, Lucie $u Department of Biomaterials and Tissue Engineering, Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., Prague, Czech Republic.
700    1_
$a Rypáček, František $u Department of Biomaterials and Bioanalogous Systems, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic v.v.i., Prague, Czech Republic.
773    0_
$w MED00180950 $t PloS one $x 1932-6203 $g Roč. 10, č. 5 (2015), s. e0125484
856    41
$u https://pubmed.ncbi.nlm.nih.gov/25945799 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20160722 $b ABA008
991    __
$a 20161216093558 $b ABA008
999    __
$a ok $b bmc $g 1155495 $s 945353
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2015 $b 10 $c 5 $d e0125484 $e 20150506 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
LZP    __
$a Pubmed-20160722

Find record

Citation metrics

Archiving options