-
Something wrong with this record ?
Characterization of Staphylococcus aureus Strains Isolated from Czech Cystic Fibrosis Patients: High Rate of Ribosomal Mutation Conferring Resistance to MLS(B) Antibiotics as a Result of Long-Term and Low-Dose Azithromycin Treatment
J. Tkadlec, E. Vařeková, R. Pantůček, J. Doškař, V. Růžičková, T. Botka, L. Fila, O. Melter,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NT12395
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
ProQuest Central
from 2000-06-01 to 2018-12-31
Health & Medicine (ProQuest)
from 2000-06-01 to 2018-12-31
Public Health Database (ProQuest)
from 2000-06-01 to 2018-12-31
PubMed
25826283
DOI
10.1089/mdr.2014.0276
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents pharmacology therapeutic use MeSH
- Azithromycin therapeutic use MeSH
- Drug Resistance, Bacterial genetics MeSH
- Cystic Fibrosis microbiology MeSH
- Long-Term Care MeSH
- Cross Infection microbiology MeSH
- Humans MeSH
- Methicillin-Resistant Staphylococcus aureus drug effects MeSH
- Microbial Sensitivity Tests MeSH
- Mutation genetics MeSH
- Ribosomes genetics MeSH
- Staphylococcal Infections drug therapy microbiology MeSH
- Staphylococcus aureus drug effects genetics MeSH
- Thymidine genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
Staphylococcus aureus is one of the most frequent pathogens infecting the respiratory tract of patients with cystic fibrosis (CF). This study was the first to examine S. aureus isolates from CF patients in the Czech Republic. Among 100 S. aureus isolates from 92 of 107 observed patients, we found a high prevalence of resistance to macrolide-lincosamide-streptogramin B (MLS(B)) antibiotics (56%). More than half of the resistant strains (29 of 56) carried a mutation in the MLS(B) target site. The emergence of MLS(B) resistance and mutations conferring resistance to MLS(B) antibiotics was associated with azithromycin treatment (p=0.000000184 and p=0.000681, respectively). Methicillin resistance was only detected in 3% of isolates and the rate of resistance to other antibiotics did not exceed 12%. The prevalence of small-colony variant (SCV) strains was relatively low (9%) and eight of nine isolates with the SCV phenotype were thymidine dependent. The study population of S. aureus was heterogeneous in structure and both the most prevalent community-associated and hospital-acquired clonal lineages were represented. Of the virulence genes, enterotoxin genes seg (n=52), sei (n=49), and sec (n=16) were the most frequently detected among the isolates. The PVL genes (lukS-PV and lukF-PV) have not been revealed in any of the isolates.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16020909
- 003
- CZ-PrNML
- 005
- 20190509102344.0
- 007
- ta
- 008
- 160722s2015 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1089/mdr.2014.0276 $2 doi
- 024 7_
- $a 10.1089/mdr.2014.0276 $2 doi
- 035 __
- $a (PubMed)25826283
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Tkadlec, Jan $u 1 Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital , Prague, Czech Republic . $7 xx0209685
- 245 10
- $a Characterization of Staphylococcus aureus Strains Isolated from Czech Cystic Fibrosis Patients: High Rate of Ribosomal Mutation Conferring Resistance to MLS(B) Antibiotics as a Result of Long-Term and Low-Dose Azithromycin Treatment / $c J. Tkadlec, E. Vařeková, R. Pantůček, J. Doškař, V. Růžičková, T. Botka, L. Fila, O. Melter,
- 520 9_
- $a Staphylococcus aureus is one of the most frequent pathogens infecting the respiratory tract of patients with cystic fibrosis (CF). This study was the first to examine S. aureus isolates from CF patients in the Czech Republic. Among 100 S. aureus isolates from 92 of 107 observed patients, we found a high prevalence of resistance to macrolide-lincosamide-streptogramin B (MLS(B)) antibiotics (56%). More than half of the resistant strains (29 of 56) carried a mutation in the MLS(B) target site. The emergence of MLS(B) resistance and mutations conferring resistance to MLS(B) antibiotics was associated with azithromycin treatment (p=0.000000184 and p=0.000681, respectively). Methicillin resistance was only detected in 3% of isolates and the rate of resistance to other antibiotics did not exceed 12%. The prevalence of small-colony variant (SCV) strains was relatively low (9%) and eight of nine isolates with the SCV phenotype were thymidine dependent. The study population of S. aureus was heterogeneous in structure and both the most prevalent community-associated and hospital-acquired clonal lineages were represented. Of the virulence genes, enterotoxin genes seg (n=52), sei (n=49), and sec (n=16) were the most frequently detected among the isolates. The PVL genes (lukS-PV and lukF-PV) have not been revealed in any of the isolates.
- 650 _2
- $a antibakteriální látky $x farmakologie $x terapeutické užití $7 D000900
- 650 _2
- $a azithromycin $x terapeutické užití $7 D017963
- 650 _2
- $a infekce spojené se zdravotní péčí $x mikrobiologie $7 D003428
- 650 _2
- $a cystická fibróza $x mikrobiologie $7 D003550
- 650 _2
- $a bakteriální léková rezistence $x genetika $7 D024881
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a dlouhodobá péče $7 D008134
- 650 _2
- $a methicilin rezistentní Staphylococcus aureus $x účinky léků $7 D055624
- 650 _2
- $a mikrobiální testy citlivosti $7 D008826
- 650 _2
- $a mutace $x genetika $7 D009154
- 650 _2
- $a ribozomy $x genetika $7 D012270
- 650 _2
- $a stafylokokové infekce $x farmakoterapie $x mikrobiologie $7 D013203
- 650 _2
- $a Staphylococcus aureus $x účinky léků $x genetika $7 D013211
- 650 _2
- $a thymidin $x genetika $7 D013936
- 651 _2
- $a Česká republika $7 D018153
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Vařeková, Eva $u Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital , Prague, Czech Republic
- 700 1_
- $a Pantůček, Roman, $u Department of Experimental Biology, Faculty of Science, Masaryk University, Brno , Czech Republic $d 1969- $7 uzp2006324651
- 700 1_
- $a Doškař, Jiří, $u Department of Experimental Biology, Faculty of Science, Masaryk University, Brno , Czech Republic $d 1951- $7 xx0076285
- 700 1_
- $a Růžičková, Vladislava, $u Department of Experimental Biology, Faculty of Science, Masaryk University, Brno , Czech Republic $d 1951- $7 mub2011664139
- 700 1_
- $a Botka, Tibor $u Department of Experimental Biology, Faculty of Science, Masaryk University, Brno , Czech Republic $7 mub2011664145
- 700 1_
- $a Fila, Libor, $u Department of Pneumology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital , Prague, Czech Republic $d 1968- $7 ola2006345307
- 700 1_
- $a Melter, Oto, $u 1 Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital , Prague, Czech Republic . $d 1965- $7 xx0102242
- 773 0_
- $w MED00008749 $t Microbial drug resistance (Larchmont, N.Y.) $x 1931-8448 $g Roč. 21, č. 4 (2015), s. 416-423
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/25826283 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20160722 $b ABA008
- 991 __
- $a 20190509102441 $b ABA008
- 999 __
- $a ok $b bmc $g 1155579 $s 945437
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 21 $c 4 $d 416-423 $e 20150331 $i 1931-8448 $m Microbial drug resistance $n Microb Drug Resist $x MED00008749
- GRA __
- $a NT12395 $p MZ0
- LZP __
- $a Pubmed-20160722