-
Je něco špatně v tomto záznamu ?
Current approaches in SELEX: An update to aptamer selection technology
M. Darmostuk, S. Rimpelova, H. Gbelcova, T. Ruml,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem, přehledy
- MeSH
- aptamerová technika SELEX metody MeSH
- aptamery nukleotidové metabolismus MeSH
- elektroforéza kapilární MeSH
- magnetismus MeSH
- metody zobrazení buněčného povrchu MeSH
- mikrosféry MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
Systematic evolution of ligands by exponential enrichment (SELEX) is a well-established and efficient technology for the generation of oligonucleotides with a high target affinity. These SELEX-derived single stranded DNA and RNA molecules, called aptamers, were selected against various targets, such as proteins, cells, microorganisms, chemical compounds etc. They have a great potential in the use as novel antibodies, in cancer theragnostics and in biomedical research. Vast interest in aptamers stimulated continuous development of SELEX, which underwent numerous modifications since its first application in 1990. Novel modifications made the selection process more efficient, cost-effective and significantly less time-consuming. This article brings a comprehensive and up-to-date review of recent advances in SELEX methods and pinpoints advantages, main obstacles and limitations. The post-SELEX strategies and examples of application are also briefly outlined in this review.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16020960
- 003
- CZ-PrNML
- 005
- 20160722121445.0
- 007
- ta
- 008
- 160722s2015 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.biotechadv.2015.02.008 $2 doi
- 024 7_
- $a 10.1016/j.biotechadv.2015.02.008 $2 doi
- 035 __
- $a (PubMed)25708387
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Darmostuk, Mariia $u Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, Prague 6 166 28, Czech Republic. Electronic address: darmosta@vscht.cz.
- 245 10
- $a Current approaches in SELEX: An update to aptamer selection technology / $c M. Darmostuk, S. Rimpelova, H. Gbelcova, T. Ruml,
- 520 9_
- $a Systematic evolution of ligands by exponential enrichment (SELEX) is a well-established and efficient technology for the generation of oligonucleotides with a high target affinity. These SELEX-derived single stranded DNA and RNA molecules, called aptamers, were selected against various targets, such as proteins, cells, microorganisms, chemical compounds etc. They have a great potential in the use as novel antibodies, in cancer theragnostics and in biomedical research. Vast interest in aptamers stimulated continuous development of SELEX, which underwent numerous modifications since its first application in 1990. Novel modifications made the selection process more efficient, cost-effective and significantly less time-consuming. This article brings a comprehensive and up-to-date review of recent advances in SELEX methods and pinpoints advantages, main obstacles and limitations. The post-SELEX strategies and examples of application are also briefly outlined in this review.
- 650 _2
- $a aptamery nukleotidové $x metabolismus $7 D052157
- 650 _2
- $a metody zobrazení buněčného povrchu $7 D061505
- 650 _2
- $a elektroforéza kapilární $7 D019075
- 650 _2
- $a magnetismus $7 D008280
- 650 _2
- $a mikrosféry $7 D008863
- 650 _2
- $a aptamerová technika SELEX $x metody $7 D052156
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 655 _2
- $a přehledy $7 D016454
- 700 1_
- $a Rimpelova, Silvie $u Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, Prague 6 166 28, Czech Republic. Electronic address: silvie.rimpelova@vscht.cz.
- 700 1_
- $a Gbelcova, Helena $u Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, Prague 6 166 28, Czech Republic; Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 4, Bratislava 811 08, Slovak Republic. Electronic address: helena.gbelcova@fmed.uniba.sk.
- 700 1_
- $a Ruml, Tomas $u Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, Prague 6 166 28, Czech Republic. Electronic address: tomas.ruml@vscht.cz.
- 773 0_
- $w MED00000793 $t Biotechnology advances $x 1873-1899 $g Roč. 33, č. 6 Pt 2 (2015), s. 1141-61
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/25708387 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20160722 $b ABA008
- 991 __
- $a 20160722121659 $b ABA008
- 999 __
- $a ok $b bmc $g 1155630 $s 945488
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2015 $b 33 $c 6 Pt 2 $d 1141-61 $e 20150220 $i 1873-1899 $m Biotechnology advances $n Biotechnol Adv $x MED00000793
- LZP __
- $a Pubmed-20160722
