-
Something wrong with this record ?
Cryptochrome 2 mediates directional magnetoreception in cockroaches
O. Bazalova, M. Kvicalova, T. Valkova, P. Slaby, P. Bartos, R. Netusil, K. Tomanova, P. Braeunig, HJ. Lee, I. Sauman, M. Damulewicz, J. Provaznik, R. Pokorny, D. Dolezel, M. Vacha,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1915 to 6 months ago
Freely Accessible Science Journals
from 1915 to 6 months ago
PubMed Central
from 1915 to 6 months ago
Europe PubMed Central
from 1915 to 6 months ago
Open Access Digital Library
from 1915-01-01
Open Access Digital Library
from 1915-01-15
- MeSH
- Phenotype MeSH
- Photoreceptor Cells, Invertebrate metabolism radiation effects MeSH
- Cryptochromes metabolism MeSH
- Magnetic Fields * MeSH
- Compound Eye, Arthropod radiation effects MeSH
- Cockroaches metabolism radiation effects MeSH
- Ultraviolet Rays MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The ability to perceive geomagnetic fields (GMFs) represents a fascinating biological phenomenon. Studies on transgenic flies have provided evidence that photosensitive Cryptochromes (Cry) are involved in the response to magnetic fields (MFs). However, none of the studies tackled the problem of whether the Cry-dependent magnetosensitivity is coupled to the sole MF presence or to the direction of MF vector. In this study, we used gene silencing and a directional MF to show that mammalian-like Cry2 is necessary for a genuine directional response to periodic rotations of the GMF vector in two insect species. Longer wavelengths of light required higher photon fluxes for a detectable behavioral response, and a sharp detection border was present in the cyan/green spectral region. Both observations are consistent with involvement of the FADox, FAD(•-) and FADH(-) redox forms of flavin. The response was lost upon covering the eyes, demonstrating that the signal is perceived in the eye region. Immunohistochemical staining detected Cry2 in the hemispherical layer of laminal glia cells underneath the retina. Together, these findings identified the eye-localized Cry2 as an indispensable component and a likely photoreceptor of the directional GMF response. Our study is thus a clear step forward in deciphering the in vivo effects of GMF and supports the interaction of underlying mechanism with the visual system.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc16027717
- 003
- CZ-PrNML
- 005
- 20161031112851.0
- 007
- ta
- 008
- 161005s2016 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1073/pnas.1518622113 $2 doi
- 024 7_
- $a 10.1073/pnas.1518622113 $2 doi
- 035 __
- $a (PubMed)26811445
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Bazalova, Olga $u Institute of Entomology, Biology Centre of Academy of Sciences of the Czech Republic, 370 05, Ceske Budejovice, Czech Republic; Department of Molecular Biology, Faculty of Science, University of South Bohemia, 370 05, Ceske Budejovice, Czech Republic;
- 245 10
- $a Cryptochrome 2 mediates directional magnetoreception in cockroaches / $c O. Bazalova, M. Kvicalova, T. Valkova, P. Slaby, P. Bartos, R. Netusil, K. Tomanova, P. Braeunig, HJ. Lee, I. Sauman, M. Damulewicz, J. Provaznik, R. Pokorny, D. Dolezel, M. Vacha,
- 520 9_
- $a The ability to perceive geomagnetic fields (GMFs) represents a fascinating biological phenomenon. Studies on transgenic flies have provided evidence that photosensitive Cryptochromes (Cry) are involved in the response to magnetic fields (MFs). However, none of the studies tackled the problem of whether the Cry-dependent magnetosensitivity is coupled to the sole MF presence or to the direction of MF vector. In this study, we used gene silencing and a directional MF to show that mammalian-like Cry2 is necessary for a genuine directional response to periodic rotations of the GMF vector in two insect species. Longer wavelengths of light required higher photon fluxes for a detectable behavioral response, and a sharp detection border was present in the cyan/green spectral region. Both observations are consistent with involvement of the FADox, FAD(•-) and FADH(-) redox forms of flavin. The response was lost upon covering the eyes, demonstrating that the signal is perceived in the eye region. Immunohistochemical staining detected Cry2 in the hemispherical layer of laminal glia cells underneath the retina. Together, these findings identified the eye-localized Cry2 as an indispensable component and a likely photoreceptor of the directional GMF response. Our study is thus a clear step forward in deciphering the in vivo effects of GMF and supports the interaction of underlying mechanism with the visual system.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a švábi $x metabolismus $x účinky záření $7 D003058
- 650 _2
- $a složené oko členovců $x účinky záření $7 D054910
- 650 _2
- $a kryptochromy $x metabolismus $7 D056931
- 650 12
- $a magnetické pole $7 D060526
- 650 _2
- $a fenotyp $7 D010641
- 650 _2
- $a fotoreceptory bezobratlých $x metabolismus $x účinky záření $7 D017956
- 650 _2
- $a ultrafialové záření $7 D014466
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Kvicalova, Marketa $u Department of Animal Physiology and Immunology, Faculty of Science, Masaryk University, 611 37, Brno, Czech Republic;
- 700 1_
- $a Valkova, Tereza $u Department of Animal Physiology and Immunology, Faculty of Science, Masaryk University, 611 37, Brno, Czech Republic;
- 700 1_
- $a Slaby, Pavel $u Department of Animal Physiology and Immunology, Faculty of Science, Masaryk University, 611 37, Brno, Czech Republic;
- 700 1_
- $a Bartos, Premysl $u Department of Animal Physiology and Immunology, Faculty of Science, Masaryk University, 611 37, Brno, Czech Republic;
- 700 1_
- $a Netusil, Radek $u Department of Animal Physiology and Immunology, Faculty of Science, Masaryk University, 611 37, Brno, Czech Republic;
- 700 1_
- $a Tomanova, Katerina $u Department of Animal Physiology and Immunology, Faculty of Science, Masaryk University, 611 37, Brno, Czech Republic;
- 700 1_
- $a Braeunig, Peter $u Department of Zoology and Animal Physiology, Institute for Biology II, RWTH Aachen University, D-52056, Aachen, Germany;
- 700 1_
- $a Lee, How-Jing $u Department of Entomology, National Taiwan University, Taipei 106, Taiwan;
- 700 1_
- $a Sauman, Ivo $u Institute of Entomology, Biology Centre of Academy of Sciences of the Czech Republic, 370 05, Ceske Budejovice, Czech Republic; Department of Molecular Biology, Faculty of Science, University of South Bohemia, 370 05, Ceske Budejovice, Czech Republic;
- 700 1_
- $a Damulewicz, Milena $u Institute of Entomology, Biology Centre of Academy of Sciences of the Czech Republic, 370 05, Ceske Budejovice, Czech Republic;
- 700 1_
- $a Provaznik, Jan $u Institute of Entomology, Biology Centre of Academy of Sciences of the Czech Republic, 370 05, Ceske Budejovice, Czech Republic; Department of Molecular Biology, Faculty of Science, University of South Bohemia, 370 05, Ceske Budejovice, Czech Republic;
- 700 1_
- $a Pokorny, Richard $u Department of Plant Physiology and Photobiology, Faculty of Biology, Philipps-University, D-35032 Marburg, Germany david.dolezel@entu.cas.cz pokorny@staff.uni-marburg.de vacha@sci.muni.cz.
- 700 1_
- $a Dolezel, David $u Institute of Entomology, Biology Centre of Academy of Sciences of the Czech Republic, 370 05, Ceske Budejovice, Czech Republic; Department of Molecular Biology, Faculty of Science, University of South Bohemia, 370 05, Ceske Budejovice, Czech Republic; david.dolezel@entu.cas.cz pokorny@staff.uni-marburg.de vacha@sci.muni.cz.
- 700 1_
- $a Vacha, Martin $u Department of Animal Physiology and Immunology, Faculty of Science, Masaryk University, 611 37, Brno, Czech Republic; david.dolezel@entu.cas.cz pokorny@staff.uni-marburg.de vacha@sci.muni.cz.
- 773 0_
- $w MED00010472 $t Proceedings of the National Academy of Sciences of the United States of America $x 1091-6490 $g Roč. 113, č. 6 (2016), s. 1660-5
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/26811445 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20161005 $b ABA008
- 991 __
- $a 20161031112815 $b ABA008
- 999 __
- $a ok $b bmc $g 1166031 $s 952347
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2016 $b 113 $c 6 $d 1660-5 $e 20160125 $i 1091-6490 $m Proceedings of the National Academy of Sciences of the United States of America $n Proc Natl Acad Sci U S A $x MED00010472
- LZP __
- $a Pubmed-20161005
