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Cytotoxicity, cell uptake and microscopic analysis of titanium dioxide and silver nanoparticles in vitro
K. Tomankova, J. Horakova, M. Harvanova, L. Malina, J. Soukupova, S. Hradilova, K. Kejlova, J. Malohlava, L. Licman, M. Dvorakova, D. Jirova, H. Kolarova,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NT14060
MZ0
CEP - Centrální evidence projektů
- MeSH
- apoptóza účinky léků MeSH
- buněčné linie účinky léků metabolismus MeSH
- buňky NIH 3T3 účinky léků MeSH
- kometový test MeSH
- kovové nanočástice chemie toxicita MeSH
- lidé MeSH
- membránový potenciál mitochondrií účinky léků MeSH
- mikroskopie atomárních sil MeSH
- myši MeSH
- Ramanova spektroskopie MeSH
- reaktivní formy kyslíku metabolismus MeSH
- spektrofotometrie atomová MeSH
- stříbro chemie farmakokinetika toxicita MeSH
- titan farmakokinetika toxicita MeSH
- velikost částic MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Commercially manufactured nanomaterials are used massively for modification of products of everyday use, including products intended for children. Therefore their potential risks have to be ultimately studied. Aside from toxicity of nanomaterials with known specific parameters, the end-consumer is potentially endangered by materials with unknown specification. Commercially available products are not usually accompanied by parameter/specification sheet providing the consumer with sufficient chemico-physical parameters allowing the evaluation of possible toxic effects. The aim of this work was to evaluate the declared parameters of commercially available TiO2 and Ag NPs employing chemico-physical methods and consequently in vitro cytotoxicity and genotoxicity tests performed on non-cancer cell lines. Based on the results of our complex study we can conclude that the data provided by the producers are not in good agreement with the performed measurements. Furthermore, all tested NPs penetrated into the SVK14 cells and all NPs had significant effect on the kinetics of ROS production in all cell lines (note: the ROS production has not been established as the major mechanism of cell damage elicited by Ag NPs). The study revealed greater cytotoxic potential of Ag NPs in comparison with TiO2 NPs and all of the studied NPs caused significant DNA damage.
Citace poskytuje Crossref.org
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- $a Tománková, Kateřina $u Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic; Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic. Electronic address: katerina.tomankova@upol.cz. $7 xx0132276
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- $a Cytotoxicity, cell uptake and microscopic analysis of titanium dioxide and silver nanoparticles in vitro / $c K. Tomankova, J. Horakova, M. Harvanova, L. Malina, J. Soukupova, S. Hradilova, K. Kejlova, J. Malohlava, L. Licman, M. Dvorakova, D. Jirova, H. Kolarova,
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- $a Commercially manufactured nanomaterials are used massively for modification of products of everyday use, including products intended for children. Therefore their potential risks have to be ultimately studied. Aside from toxicity of nanomaterials with known specific parameters, the end-consumer is potentially endangered by materials with unknown specification. Commercially available products are not usually accompanied by parameter/specification sheet providing the consumer with sufficient chemico-physical parameters allowing the evaluation of possible toxic effects. The aim of this work was to evaluate the declared parameters of commercially available TiO2 and Ag NPs employing chemico-physical methods and consequently in vitro cytotoxicity and genotoxicity tests performed on non-cancer cell lines. Based on the results of our complex study we can conclude that the data provided by the producers are not in good agreement with the performed measurements. Furthermore, all tested NPs penetrated into the SVK14 cells and all NPs had significant effect on the kinetics of ROS production in all cell lines (note: the ROS production has not been established as the major mechanism of cell damage elicited by Ag NPs). The study revealed greater cytotoxic potential of Ag NPs in comparison with TiO2 NPs and all of the studied NPs caused significant DNA damage.
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- $a Soukupová, Jana $u Department of Physical Chemistry, Faculty of Science, Regional Centre of Advanced Technologies and Materials, Slechtitelu 11, 783 71 Olomouc, Czech Republic. $7 _AN056459
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- $a Kolářová, Hana, $u Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic; Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic. $d 1961- $7 _gn020022061
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