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A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics

S. Tacke, V. Krzyzanek, H. Nüsse, RA. Wepf, J. Klingauf, R. Reichelt,

. 2016 ; 110 (4) : 758-65.

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc17000415
E-zdroje Online Plný text

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Odkazy

PubMed 26910419
DOI 10.1016/j.bpj.2016.01.024
Knihovny.cz E-zdroje

Cryogenic microscopy methods have gained increasing popularity, as they offer an unaltered view on the architecture of biological specimens. As a prerequisite, samples must be handled under cryogenic conditions below their recrystallization temperature, and contamination during sample transfer and handling must be prevented. We present a high-vacuum cryo-transfer system that streamlines the entire handling of frozen-hydrated samples from the vitrification process to low temperature imaging for scanning transmission electron microscopy and transmission electron microscopy. A template for cryo-electron microscopy and multimodal cryo-imaging approaches with numerous sample transfer steps is presented.

Citace poskytuje Crossref.org

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