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Metallohelix vectors for efficient gene delivery via cationic DNA nanoparticles
J. Malina, H. Kostrhunova, V. Novohradsky, P. Scott, V. Brabec
Language English Country Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
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PubMed
35018455
DOI
10.1093/nar/gkab1277
Knihovny.cz E-resources
- MeSH
- Cell Line MeSH
- DNA chemistry ultrastructure MeSH
- Gene Expression MeSH
- Fluorescent Antibody Technique MeSH
- Genetic Vectors * chemistry ultrastructure MeSH
- Cations chemistry MeSH
- Metal Nanoparticles chemistry ultrastructure MeSH
- Humans MeSH
- Microscopy, Atomic Force methods MeSH
- Molecular Structure MeSH
- Flow Cytometry MeSH
- Genes, Reporter MeSH
- Gene Transfer Techniques * MeSH
- Transfection MeSH
- Cell Survival MeSH
- Ferrous Compounds chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The design of efficient and safe gene delivery vehicles remains a major challenge for the application of gene therapy. Of the many reported gene delivery systems, metal complexes with high affinity for nucleic acids are emerging as an attractive option. We have discovered that certain metallohelices-optically pure, self-assembling triple-stranded arrays of fully encapsulated Fe-act as nonviral DNA delivery vectors capable of mediating efficient gene transfection. They induce formation of globular DNA particles which protect the DNA from degradation by various restriction endonucleases, are of suitable size and electrostatic potential for efficient membrane transport and are successfully processed by cells. The activity is highly structure-dependent-compact and shorter metallohelix enantiomers are far less efficient than less compact and longer enantiomers.
Czech Academy of Sciences Institute of Biophysics Brno CZ 61265 Czech Republic
Department of Chemistry University of Warwick Coventry CV4 7AL UK
References provided by Crossref.org
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