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Metallohelix vectors for efficient gene delivery via cationic DNA nanoparticles
J. Malina, H. Kostrhunova, V. Novohradsky, P. Scott, V. Brabec
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
Free Medical Journals od 1996
PubMed Central od 1974
Europe PubMed Central od 1974
Open Access Digital Library od 1996-01-01 do 2030-12-31
Open Access Digital Library od 1974-01-01
Open Access Digital Library od 1996-01-01
Open Access Digital Library od 1996-01-01
Medline Complete (EBSCOhost) od 1996-01-01
Oxford Journals Open Access Collection od 1996-01-01
ROAD: Directory of Open Access Scholarly Resources od 1974
Odkazy
PubMed
35018455
DOI
10.1093/nar/gkab1277
Knihovny.cz E-zdroje
- MeSH
- buněčné linie MeSH
- DNA chemie ultrastruktura MeSH
- exprese genu MeSH
- fluorescenční protilátková technika MeSH
- genetické vektory * chemie ultrastruktura MeSH
- kationty chemie MeSH
- kovové nanočástice chemie ultrastruktura MeSH
- lidé MeSH
- mikroskopie atomárních sil metody MeSH
- molekulární struktura MeSH
- průtoková cytometrie MeSH
- reportérové geny MeSH
- technika přenosu genů * MeSH
- transfekce MeSH
- viabilita buněk MeSH
- železnaté sloučeniny chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The design of efficient and safe gene delivery vehicles remains a major challenge for the application of gene therapy. Of the many reported gene delivery systems, metal complexes with high affinity for nucleic acids are emerging as an attractive option. We have discovered that certain metallohelices-optically pure, self-assembling triple-stranded arrays of fully encapsulated Fe-act as nonviral DNA delivery vectors capable of mediating efficient gene transfection. They induce formation of globular DNA particles which protect the DNA from degradation by various restriction endonucleases, are of suitable size and electrostatic potential for efficient membrane transport and are successfully processed by cells. The activity is highly structure-dependent-compact and shorter metallohelix enantiomers are far less efficient than less compact and longer enantiomers.
Czech Academy of Sciences Institute of Biophysics Brno CZ 61265 Czech Republic
Department of Chemistry University of Warwick Coventry CV4 7AL UK
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