-
Je něco špatně v tomto záznamu ?
Characterization of three distinct metallothionein genes of the Ag-hyperaccumulating ectomycorrhizal fungus Amanita strobiliformis
K. Hložková, M. Matěnová, P. Žáčková, H. Strnad, H. Hršelová, M. Hroudová, P. Kotrba,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- Amanita genetika metabolismus MeSH
- genetická transkripce účinky léků MeSH
- měď metabolismus MeSH
- metalothionein genetika metabolismus MeSH
- Saccharomyces cerevisiae účinky léků genetika metabolismus MeSH
- stanovení celkové genové exprese MeSH
- stříbro metabolismus MeSH
- tolerance léku MeSH
- zinek metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Mechanisms evolved in eukaryotes to handle heavy metals involve cytosolic, metal-binding metallothioneins (MTs). We have previously documented that the sequestration of silver (Ag) in the Ag-hyperaccumulating Amanita strobiliformis is dominated by 34-amino-acid (AA) AsMT1a, 1b, and 1c isoforms. Here we show that in addition to AsMT1a, 1b, and 1c isogenes, the fungus has two other MT genes: AsMT2 encoding a 34-AA AsMT2 similar to MTs known from other species, but unrelated to AsMT1s; AsMT3 coding for a 62-AA AsMT3 that shares substantial identity with as-yet-uncharacterized conserved peptides predicted in agaricomycetes. Transcription of AsMT1s and AsMT3 in the A. strobiliformis mycelium was specifically inducible by treatments with Ag or copper (Cu) and zinc (Zn) or cadmium (Cd), respectively; AsMT2 showed a moderate upregulation in the presence of Cd. Expression of AsMTs in the metal-sensitive Saccharomyces cerevisiae revealed that all AsMTs confer increased Cd tolerance (AsMT3 proved the most effective) and that, unlike AsMT1 and AsMT2, AsMT3 can protect the yeasts against Zn toxicity. The highest level of Cu tolerance was observed with yeasts expressing AsMT1a. Our data indicate that A. strobiliformis can specifically employ different MT genes for functions in the cellular handling of Ag and Cu (AsMT1s) and Zn (AsMT3).
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc17000435
- 003
- CZ-PrNML
- 005
- 20170117093151.0
- 007
- ta
- 008
- 170103s2016 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.funbio.2015.11.007 $2 doi
- 024 7_
- $a 10.1016/j.funbio.2015.11.007 $2 doi
- 035 __
- $a (PubMed)26895864
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Hložková, Kateřina $u Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 3, CZ-166 28 Prague, Czech Republic.
- 245 10
- $a Characterization of three distinct metallothionein genes of the Ag-hyperaccumulating ectomycorrhizal fungus Amanita strobiliformis / $c K. Hložková, M. Matěnová, P. Žáčková, H. Strnad, H. Hršelová, M. Hroudová, P. Kotrba,
- 520 9_
- $a Mechanisms evolved in eukaryotes to handle heavy metals involve cytosolic, metal-binding metallothioneins (MTs). We have previously documented that the sequestration of silver (Ag) in the Ag-hyperaccumulating Amanita strobiliformis is dominated by 34-amino-acid (AA) AsMT1a, 1b, and 1c isoforms. Here we show that in addition to AsMT1a, 1b, and 1c isogenes, the fungus has two other MT genes: AsMT2 encoding a 34-AA AsMT2 similar to MTs known from other species, but unrelated to AsMT1s; AsMT3 coding for a 62-AA AsMT3 that shares substantial identity with as-yet-uncharacterized conserved peptides predicted in agaricomycetes. Transcription of AsMT1s and AsMT3 in the A. strobiliformis mycelium was specifically inducible by treatments with Ag or copper (Cu) and zinc (Zn) or cadmium (Cd), respectively; AsMT2 showed a moderate upregulation in the presence of Cd. Expression of AsMTs in the metal-sensitive Saccharomyces cerevisiae revealed that all AsMTs confer increased Cd tolerance (AsMT3 proved the most effective) and that, unlike AsMT1 and AsMT2, AsMT3 can protect the yeasts against Zn toxicity. The highest level of Cu tolerance was observed with yeasts expressing AsMT1a. Our data indicate that A. strobiliformis can specifically employ different MT genes for functions in the cellular handling of Ag and Cu (AsMT1s) and Zn (AsMT3).
- 650 _2
- $a Amanita $x genetika $x metabolismus $7 D000545
- 650 _2
- $a měď $x metabolismus $7 D003300
- 650 _2
- $a tolerance léku $7 D004361
- 650 _2
- $a stanovení celkové genové exprese $7 D020869
- 650 _2
- $a metalothionein $x genetika $x metabolismus $7 D008668
- 650 _2
- $a Saccharomyces cerevisiae $x účinky léků $x genetika $x metabolismus $7 D012441
- 650 _2
- $a stříbro $x metabolismus $7 D012834
- 650 _2
- $a genetická transkripce $x účinky léků $7 D014158
- 650 _2
- $a zinek $x metabolismus $7 D015032
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Matěnová, Michaela $u Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 3, CZ-166 28 Prague, Czech Republic.
- 700 1_
- $a Žáčková, Petra $u Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 3, CZ-166 28 Prague, Czech Republic.
- 700 1_
- $a Strnad, Hynek $u Institute of Molecular Genetics, v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-142 20 Prague 4, Czech Republic.
- 700 1_
- $a Hršelová, Hana $u Institute of Microbiology, v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-142 20 Prague 4, Czech Republic.
- 700 1_
- $a Hroudová, Miluše $u Institute of Molecular Genetics, v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-142 20 Prague 4, Czech Republic.
- 700 1_
- $a Kotrba, Pavel $u Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 3, CZ-166 28 Prague, Czech Republic. Electronic address: pavel.kotrba@vscht.cz.
- 773 0_
- $w MED00180227 $t Fungal biology $x 1878-6146 $g Roč. 120, č. 3 (2016), s. 358-69
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/26895864 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20170103 $b ABA008
- 991 __
- $a 20170117093257 $b ABA008
- 999 __
- $a ok $b bmc $g 1179575 $s 961002
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2016 $b 120 $c 3 $d 358-69 $e 20151126 $i 1878-6146 $m Fungal biology $n Fungal Biol $x MED00180227
- LZP __
- $a Pubmed-20170103
