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Crystallization of nepenthesin I using a low-pH crystallization screen
K. Fejfarová, A. Kádek, H. Mrázek, J. Hausner, V. Tretyachenko, T. Koval', P. Man, J. Hašek, J. Dohnálek,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 2014 to 2 years ago
PubMed Central
from 2014 to 1 year ago
Europe PubMed Central
from 2014 to 2 years ago
- MeSH
- Aspartic Acid Endopeptidases chemistry MeSH
- Hydrogen-Ion Concentration MeSH
- Crystallization MeSH
- Crystallography, X-Ray MeSH
- Magnoliopsida enzymology MeSH
- Pepstatins chemistry MeSH
- Plant Proteins chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Nepenthesins are aspartic proteases secreted by carnivorous pitcher plants of the genus Nepenthes. They significantly differ in sequence from other plant aspartic proteases. This difference, which provides more cysteine residues in the structure of nepenthesins, may contribute to their unique stability profile. Recombinantly produced nepenthesin 1 (rNep1) from N. gracilis in complex with pepstatin A was crystallized under two different crystallization conditions using a newly formulated low-pH crystallization screen. The diffraction data were processed to 2.9 and 2.8 Å resolution, respectively. The crystals belonged to space group P212121, with unit-cell parameters a = 86.63, b = 95.90, c = 105.40 Å, α = β = γ = 90° and a = 86.28, b = 97.22, c = 103.78 Å, α = β = γ = 90°, respectively. Matthews coefficient and solvent-content calculations suggest the presence of two molecules of rNep1 in the asymmetric unit. Here, the details of the crystallization experiment and analysis of the X-ray data are reported.
Faculty of Science Charles University Prague Albertov 6 128 44 Praha 2 Czech Republic
Institute of Biotechnology CAS v v i Vídeňská 1083 142 20 Praha 4 Czech Republic
Institute of Macromolecular Chemistry CAS v v i Heyrovského nám 2 1888 162 06 Praha 6 Czech Republic
Institute of Microbiology CAS v v i Vídeňská 1083 142 20 Praha 4 Czech Republic
References provided by Crossref.org
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