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Caspases and osteogenic markers--in vitro screening of inhibition impact
E. Adamova, E. Janeckova, K. Kleparnik, E. Matalova,
Language English Country Germany
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
ProQuest Central
from 1998-01-01 to 2018-12-31
Medline Complete (EBSCOhost)
from 2011-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 1998-01-01 to 2018-12-31
Public Health Database (ProQuest)
from 1998-01-01 to 2018-12-31
- MeSH
- Apoptosis drug effects MeSH
- Cell Differentiation drug effects MeSH
- Chondrogenesis drug effects MeSH
- Caspase Inhibitors administration & dosage MeSH
- Caspase 3 genetics metabolism MeSH
- Mesenchymal Stem Cells drug effects MeSH
- Mice MeSH
- Osteogenesis drug effects MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Caspases, well-known players in apoptosis or inflammation, appear to have roles also in other processes such as cell differentiation. Caspase-3, in particular, was recently demonstrated to have non-apoptotic functions in osteogenesis. However, the molecular pathways involved are not yet known. Therefore, we used osteogenic PCR arrays to provide a comprehensive screening of possible interactions of caspases in general and specifically of caspase-3 in osteogenic networks. Embryonic micromass cultures derived from mouse forelimbs were established and pharmacological fluoromethylketone (FMK) inhibitors applied. Alterations were observed in expression of several genes after caspase inhibition (Bmp1, Bmp5, Bmp6, Col10a1, Col2a1, Comp, Egf, Fgfr2, Gli1, Igf1, Nog, Phex, Sox9, Spp1). The list suggests molecular interactions of caspases and osteogenic molecules and creates a background for further temporospatial and functional studies.
References provided by Crossref.org
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