Caspases and osteogenic markers--in vitro screening of inhibition impact
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26512002
DOI
10.1007/s11626-015-9964-1
PII: 10.1007/s11626-015-9964-1
Knihovny.cz E-zdroje
- Klíčová slova
- Caspase-3, Caspases, Chondrogenesis, Gene expression, Osteogenesis,
- MeSH
- apoptóza účinky léků MeSH
- buněčná diferenciace účinky léků MeSH
- chondrogeneze účinky léků MeSH
- inhibitory kaspas aplikace a dávkování MeSH
- kaspasa 3 genetika metabolismus MeSH
- mezenchymální kmenové buňky účinky léků MeSH
- myši MeSH
- osteogeneze účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- inhibitory kaspas MeSH
- kaspasa 3 MeSH
Caspases, well-known players in apoptosis or inflammation, appear to have roles also in other processes such as cell differentiation. Caspase-3, in particular, was recently demonstrated to have non-apoptotic functions in osteogenesis. However, the molecular pathways involved are not yet known. Therefore, we used osteogenic PCR arrays to provide a comprehensive screening of possible interactions of caspases in general and specifically of caspase-3 in osteogenic networks. Embryonic micromass cultures derived from mouse forelimbs were established and pharmacological fluoromethylketone (FMK) inhibitors applied. Alterations were observed in expression of several genes after caspase inhibition (Bmp1, Bmp5, Bmp6, Col10a1, Col2a1, Comp, Egf, Fgfr2, Gli1, Igf1, Nog, Phex, Sox9, Spp1). The list suggests molecular interactions of caspases and osteogenic molecules and creates a background for further temporospatial and functional studies.
Department of Experimental Biology Faculty of Science Masaryk University Brno Czech Republic
Department of Physiology University of Veterinary and Pharmaceutical Sciences Brno Czech Republic
Institute of Analytical Chemistry v v i Brno Czech Republic
Institute of Animal Physiology and Genetics CAS v v i Brno Czech Republic
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