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Expression of p21 and expression and activation of mapk regulated transcription factors in peripheral blood mononuclear cells in rats after whole-body γ-radiation and its use in biodosimetry
Jaroslav Pejchal, Sarka Pruchova, Klara Kubelkova, Ales Tichy, Jirina Vavrova
Jazyk angličtina Země Česko
Typ dokumentu práce podpořená grantem
Digitální knihovna NLK
Zdroj
NLK
ROAD: Directory of Open Access Scholarly Resources
od 2011
- MeSH
- aktivační transkripční faktory * MeSH
- biologické markery krev MeSH
- časové faktory MeSH
- celotělové ozáření MeSH
- ELISA MeSH
- inhibitor p21 cyklin-dependentní kinasy metabolismus účinky záření MeSH
- ionizující záření * MeSH
- mitogenem aktivované proteinkinasy kinas metabolismus účinky záření MeSH
- potkani Wistar MeSH
- protein vázající cAMP responzivní element metabolismus účinky záření MeSH
- protoonkogenní proteiny c-myc metabolismus účinky záření MeSH
- radiometrie statistika a číselné údaje MeSH
- vztah dávky záření a odpovědi MeSH
- western blotting MeSH
- záření gama MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
The aim of our study was to examine the in vivo expression of p21 and expression and activation of ATF-2, c-Myc, and CREB in rat peripheral blood mononuclear cells (PBMC) after whole body γ-irradiation and to assess its contribution to biodosimetry. For Western blot experiments, male Wistar rats were whole-body irradiated by a single dose of 0, 0.5, 1, 3, and 5 Gy (60 Co, 1 m, 0.7 Gy/min). As a positive control, leukaemic MOLT-4 cells were used. For ELISA experiments, male Wistar rats were whole-body irradiated by a single dose of 0, 1, 2, 3, 4, and 5 Gy (60 Co, 1 m, 0.6 Gy/min). Blood samples were taken 4 h after the irradiation and PBMC were isolated using centrifugation on Histopaque-1077. Expressions of p21, ATF-2, phospho-ATF-2Thr69/71 , c-Myc, phospho-c-MycThr-58/Ser62 , CREB, and phospho-CREBSer133 were measured using Western blot method. Expression of p21 was also quantified using ELISA. We observed increase of p21 expression in rat PBMC 4 h after irradiation. According to ELISA, p21 levels increased 2.0-, 3.1-, 5.5-, 3.0-, and 3.1fold after irradiation by 1, 2, 3, 4, and 5 Gy, respectively. We did not detect any expression or activation of ATF-2, c-Myc and CREB. Protein p21 could be considered as a perspective biodosimetric marker of clinically significant irradiation (≥1 Gy) in vivo in unstimulated PBMC.
Citace poskytuje Crossref.org
Literatura
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- $a The aim of our study was to examine the in vivo expression of p21 and expression and activation of ATF-2, c-Myc, and CREB in rat peripheral blood mononuclear cells (PBMC) after whole body γ-irradiation and to assess its contribution to biodosimetry. For Western blot experiments, male Wistar rats were whole-body irradiated by a single dose of 0, 0.5, 1, 3, and 5 Gy (60 Co, 1 m, 0.7 Gy/min). As a positive control, leukaemic MOLT-4 cells were used. For ELISA experiments, male Wistar rats were whole-body irradiated by a single dose of 0, 1, 2, 3, 4, and 5 Gy (60 Co, 1 m, 0.6 Gy/min). Blood samples were taken 4 h after the irradiation and PBMC were isolated using centrifugation on Histopaque-1077. Expressions of p21, ATF-2, phospho-ATF-2Thr69/71 , c-Myc, phospho-c-MycThr-58/Ser62 , CREB, and phospho-CREBSer133 were measured using Western blot method. Expression of p21 was also quantified using ELISA. We observed increase of p21 expression in rat PBMC 4 h after irradiation. According to ELISA, p21 levels increased 2.0-, 3.1-, 5.5-, 3.0-, and 3.1fold after irradiation by 1, 2, 3, 4, and 5 Gy, respectively. We did not detect any expression or activation of ATF-2, c-Myc and CREB. Protein p21 could be considered as a perspective biodosimetric marker of clinically significant irradiation (≥1 Gy) in vivo in unstimulated PBMC.
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