-
Something wrong with this record ?
Selective effect of irreversible electroporation on parenchyma of the pancreas and its vascular structures - an in vivo experiment on a porcine model
Roman Svatoň, Jan Hlavsa, Zdeněk Kala, Vladimír Procházka, Katarína Gašparová, Michal Crha, Alois Nečas, Petr Raušer, Tomáš Andrašina, Vladimír Válek, Iva Svobodová
Language English Country Czech Republic
Document type Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1978
Open Access Digital Library
from 1978-01-01
ROAD: Directory of Open Access Scholarly Resources
from 1978
- MeSH
- Ablation Techniques methods MeSH
- Blood Vessels * pathology MeSH
- Electrochemotherapy * methods instrumentation adverse effects MeSH
- Animal Experimentation MeSH
- Laparotomy MeSH
- Models, Animal MeSH
- Necrosis etiology MeSH
- Pancreas * anatomy & histology blood supply pathology MeSH
- Postoperative Complications MeSH
- Postoperative Period MeSH
- Swine MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
Irreversible electroporation is a local, non-thermal ablation method, where short electrical pulses of high voltage lead to changes in cell membrane permeability and cell death. Recent experimental studies have shown that it does not lead to damage of blood vessels, nerves, bile duct or ureters. The aim of our experimental study was to evaluate the negative effect of irreversible electroporation regarding damage to the vascular wall and porcine pancreatic tissue. Irreversible electroporation of the pancreas was performed in 6 pigs after medial laparotomy. Irreversible electroporation was applied to each pig to the splenic lobe of the pancreas in order to assess damage to the pancreatic tissue and to the duodenal lobe of the pancreas to assess damage to the vascular structure of the pancreatic tissue. Higher ablation electric intensity (minimum 500 V/cm – maximum 1,750 V/cm, step 250 V/cm) in 90 μs pulses was utilized on each pig. After 7 days, macroscopic and microscopic evaluations of en bloc resected specimen (pancreas with duodenum) were performed. During 7 post-ablation days, no deaths or clinical worsening occurred in any of the pigs. Necrotic changes in the pancreatic tissue were recorded at an electric intensity of 750 V/cm. Changes in the outer layers of the wall of the arteries and veins occurred at 1,000 V/cm. Transmural vascular wall damage was not recorded in any case. Irreversible electroporation allows for relatively efficient cell death in the target tissues. Our independent experimental work confirms the safety of this method towards vascular structures located in the ablation zone
St Anne's University Hospital Brno Department of Pathology Brno Czech Republic
University Hospital and Masaryk University Brno Department of Radiology Brno Czech Republic
University Hospital and Masaryk University Brno Department of Surgery Brno Czech Republic
References provided by Crossref.org
Literatura
- 000
- 00000naa a2200000 a 4500
- 001
- bmc17011457
- 003
- CZ-PrNML
- 005
- 20230802115127.0
- 007
- cr|cn|
- 008
- 170331s2016 xr a fs 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.2754/avb201685020133 $2 doi
- 040 __
- $a ABA008 $d ABA008 $e AACR2 $b cze
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Svatoň, Roman $7 xx0108126 $u University Hospital and Masaryk University Brno, Department of Surgery, Brno, Czech Republic
- 245 10
- $a Selective effect of irreversible electroporation on parenchyma of the pancreas and its vascular structures - an in vivo experiment on a porcine model / $c Roman Svatoň, Jan Hlavsa, Zdeněk Kala, Vladimír Procházka, Katarína Gašparová, Michal Crha, Alois Nečas, Petr Raušer, Tomáš Andrašina, Vladimír Válek, Iva Svobodová
- 504 __
- $a Literatura
- 520 3_
- $a Irreversible electroporation is a local, non-thermal ablation method, where short electrical pulses of high voltage lead to changes in cell membrane permeability and cell death. Recent experimental studies have shown that it does not lead to damage of blood vessels, nerves, bile duct or ureters. The aim of our experimental study was to evaluate the negative effect of irreversible electroporation regarding damage to the vascular wall and porcine pancreatic tissue. Irreversible electroporation of the pancreas was performed in 6 pigs after medial laparotomy. Irreversible electroporation was applied to each pig to the splenic lobe of the pancreas in order to assess damage to the pancreatic tissue and to the duodenal lobe of the pancreas to assess damage to the vascular structure of the pancreatic tissue. Higher ablation electric intensity (minimum 500 V/cm – maximum 1,750 V/cm, step 250 V/cm) in 90 μs pulses was utilized on each pig. After 7 days, macroscopic and microscopic evaluations of en bloc resected specimen (pancreas with duodenum) were performed. During 7 post-ablation days, no deaths or clinical worsening occurred in any of the pigs. Necrotic changes in the pancreatic tissue were recorded at an electric intensity of 750 V/cm. Changes in the outer layers of the wall of the arteries and veins occurred at 1,000 V/cm. Transmural vascular wall damage was not recorded in any case. Irreversible electroporation allows for relatively efficient cell death in the target tissues. Our independent experimental work confirms the safety of this method towards vascular structures located in the ablation zone
- 650 _2
- $a zvířata $7 D000818
- 650 12
- $a elektrochemoterapie $x metody $x přístrojové vybavení $x škodlivé účinky $7 D053672
- 650 _2
- $a prasata $7 D013552
- 650 _2
- $a ablace $x metody $7 D055011
- 650 12
- $a cévy $x patologie $7 D001808
- 650 _2
- $a experimenty na zvířatech $7 D032761
- 650 _2
- $a laparotomie $7 D007813
- 650 _2
- $a pooperační období $7 D011184
- 650 _2
- $a nekróza $x etiologie $7 D009336
- 650 _2
- $a pooperační komplikace $7 D011183
- 650 _2
- $a modely u zvířat $7 D023421
- 650 12
- $a pankreas $x anatomie a histologie $x krevní zásobení $x patologie $7 D010179
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Hlavsa, Jan $7 xx0158100 $u University Hospital and Masaryk University Brno, Department of Surgery, Brno, Czech Republic
- 700 1_
- $a Kala, Zdeněk, $d 1960- $7 xx0003702 $u University Hospital and Masaryk University Brno, Department of Surgery, Brno, Czech Republic
- 700 1_
- $a Procházka, Vladimír, $d 1972- $7 xx0070644 $u University Hospital and Masaryk University Brno, Department of Surgery, Brno, Czech Republic
- 700 1_
- $a Gašparová, Katarína $7 _AN090959 $u University Hospital and Masaryk University Brno, Department of Surgery, Brno, Czech Republic
- 700 1_
- $a Crha, Michal $7 xx0158096 $u University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Department of Surgery and Orthopedics, Small Animal Clinic, Brno, Czech Republic
- 700 1_
- $a Nečas, Alois, $d 1966- $7 mzk2004229096 $u University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Department of Surgery and Orthopedics, Small Animal Clinic, Brno, Czech Republic
- 700 1_
- $a Raušer, Petr, $d 1972- $7 mzk2004221783 $u University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Department of Surgery and Orthopedics, Small Animal Clinic, Brno, Czech Republic
- 700 1_
- $a Andrašina, Tomáš $7 xx0074370 $u University Hospital and Masaryk University Brno, Department of Radiology, Brno, Czech Republic
- 700 1_
- $a Válek, Vlastimil, $d 1960- $7 nlk20010098982 $u University Hospital and Masaryk University Brno, Department of Radiology, Brno, Czech Republic
- 700 1_
- $a Svobodová, Iva $7 xx0128976 $u St. Anne's University Hospital Brno, Department of Pathology, Brno, Czech Republic
- 773 0_
- $t Acta veterinaria Brno $x 0001-7213 $g Roč. 85, č. 2 (2016), s. 133-137 $w MED00172332
- 910 __
- $a ABA008 $b online $y 4 $z 0
- 990 __
- $a 20170331101410 $b ABA008
- 991 __
- $a 20230802115123 $b ABA008
- 999 __
- $a ok $b bmc $g 1196772 $s 972201
- BAS __
- $a 3 $a 4
- BMC __
- $a 2016 $b 85 $c 2 $d 133-137 $i 0001-7213 $m Acta veterinaria Brno $x MED00172332
- LZP __
- $c NLK185 $d 20190125 $a NLK 2017-11/pk
