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Rapid diagnostic testing of toxigenic microfungi isolated from foodstuffs
Marie Jefremova, Vladimír Ostrý, František Malíř, Jiří Ruprich
Language English Country Czech Republic
Document type Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1978
Open Access Digital Library
from 1978-01-01
ROAD: Directory of Open Access Scholarly Resources
from 1978
- MeSH
- Food Analysis MeSH
- Aspergillus isolation & purification classification pathogenicity MeSH
- Food Safety MeSH
- Chromogenic Compounds MeSH
- Indicators and Reagents MeSH
- Animal Feed MeSH
- Culture Media * classification MeSH
- Microbiological Techniques * methods MeSH
- Mitosporic Fungi * isolation & purification classification metabolism pathogenicity MeSH
- Mycotoxins biosynthesis isolation & purification classification adverse effects MeSH
- Penicillium isolation & purification classification pathogenicity MeSH
- Food Microbiology MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
The aim of this study was to experimentally test chromogenic media and reagents for a rapid diagnosis of toxigenic microfungi Aspergillus flavus, A. carbonarius, A. ochraceus, Penicillium expansum, P. verrucosum, and P. citrinum isolated from foodstuffs. Toxigenic microfungi of Aspergillus and Penicillium species are considered to be of major relevance for human and animal health. The presence of these toxigenic microfungi in foodstuffs and feedstuffs raises a potential risk of mycotoxins (for example presence of aflatoxins, cyclopiazonic acid, ochratoxin A, patulin or citrinin). In order to reduce the risks for consumers’ health, it is necessary to set rapid methods for toxigenic microfungi isolated from foodstuffs and also feedstuffs. Application of chromogenic media and reagents presents one of the possibilities. The selected strains of toxigenic microfungi, producing mycotoxins, were tested on chromogenic media. The chromogenic media and reagents in our study are suitable for a microbiological and mycological laboratory. The benefits of chromogenic media and reagents include a rapid diagnosis within 48–72 h, simple preparation, easy availability and relatively low prices.
National Institute of Public Health Prague Centre for Health Nutrition and Food Brno Czech Republic
University of Hradec Králové Faculty of Science Department of Biology Hradec Králové Czech Republic
References provided by Crossref.org
Literatura
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