The effect of the combination of α-pinene and β-caryophyllene on the proliferation of the Trypanosoma evansi was analyzed. These compounds were added individually and in combination at different concentrations to T. evansi cultures, and the in vivo study used mice of 1.0 and 1.5 mL kg−1 of α-pinene and β-caryophyllene individually and in combination against T. evansi. In order to evaluate the in vitro safety, lymphocytes and VERO cells were assessed using tetrazolium salt (MTT) assay. The trypanocidal effect of α-pinene and β-caryophyllene was observed in vitro when applied independently or combined at 0.5, 1 and 2% concentrations. The combination effect showed a faster trypanocidal effect when compared to chemotherapy (diminazene aceturate – D.A.). Concerning the in vivo evaluation, α-pinene + β-caryophyllene at 1.0 mL kg−1 was able to extend animal longevity and showed 83.33% curative efficacy, a superior result when compared to D.A. (16.66%). The MTT assay showed that the assessed compounds do not present toxicity to used cells. Also, in vivo safety evaluation of α-pinene + β-caryophyllene at 1.0 mL kg−1 not altered the aminotransferases levels in serum. Combination between α-pinene and β-caryophyllene shows in vitro and in vivo trypanocidal activity against T. evansi, in a safe dose.

Department of Animal Science Universidade do Estado de Santa Catarina Chapecó SC Brazil

Department of Microbiology and Parasitology Universidade Federal de Santa Maria Santa Maria RS Brazi

Department of Microbiology and Parasitology Universidade Federal de Santa Maria Santa Maria RS Brazil

Laboratory of Cell Culture Centro Universitário Franciscano Santa Maria RS Brazil

Literatura

000      

00000naa a2200000 a 4500

001      

bmc17012153

003      

CZ-PrNML

005      

20170531102932.0

007      

ta

008      

170407s2016 xr d f 000 0|eng||

009      

AR

024    7_

$a 10.1016/j.jab.2016.04.004 $2 doi

040    __

$a ABA008 $d ABA008 $e AACR2 $b cze

041    0_

$a eng

044    __

$a xr

100    1_

$a Amaral, Renata G. $u Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil $7 gn_A_00005270

245    10

$a Combination of the essential oil constituents α-pinene and β-caryophyllene as a potentiator of trypanocidal action on Trypanosoma evansi / $c Renata G. Amaral, Matheus D. Baldissera, Thirssa H. Grando, Jéssica C.M. Couto, Christopher P. Posser, Andiara P. Ramos, Michele R. Sagrillo, Rodrigo A. Vaucher, Aleksandro S. Da Silva, Ana P. Becker, Silvia G. Monteiro

504    __

$a Literatura

520    9_

$a The effect of the combination of α-pinene and β-caryophyllene on the proliferation of the Trypanosoma evansi was analyzed. These compounds were added individually and in combination at different concentrations to T. evansi cultures, and the in vivo study used mice of 1.0 and 1.5 mL kg−1 of α-pinene and β-caryophyllene individually and in combination against T. evansi. In order to evaluate the in vitro safety, lymphocytes and VERO cells were assessed using tetrazolium salt (MTT) assay. The trypanocidal effect of α-pinene and β-caryophyllene was observed in vitro when applied independently or combined at 0.5, 1 and 2% concentrations. The combination effect showed a faster trypanocidal effect when compared to chemotherapy (diminazene aceturate – D.A.). Concerning the in vivo evaluation, α-pinene + β-caryophyllene at 1.0 mL kg−1 was able to extend animal longevity and showed 83.33% curative efficacy, a superior result when compared to D.A. (16.66%). The MTT assay showed that the assessed compounds do not present toxicity to used cells. Also, in vivo safety evaluation of α-pinene + β-caryophyllene at 1.0 mL kg−1 not altered the aminotransferases levels in serum. Combination between α-pinene and β-caryophyllene shows in vitro and in vivo trypanocidal activity against T. evansi, in a safe dose.

650    _2

$a Trypanosoma $x izolace a purifikace $x účinky léků $7 D014345

650    02

$a trypanocidální látky $x aplikace a dávkování $7 D014344

650    _2

$a monoterpeny $x aplikace a dávkování $x farmakologie $x chemie $7 D039821

650    _2

$a seskviterpeny $x aplikace a dávkování $x farmakologie $x chemie $7 D012717

650    _2

$a vztahy mezi strukturou a aktivitou $7 D013329

650    _2

$a modely u zvířat $7 D023421

650    _2

$a zvířata $7 D000818

650    _2

$a myši $7 D051379

650    _2

$a ženské pohlaví $7 D005260

700    1_

$a Baldissera, Matheus D. $u Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazi

700    1_

$a Grando, Thirssa H. $u Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazi

700    1_

$a Couto, Jéssica C.M. $u Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazi

700    1_

$a Posser, Christopher P. $u Laboratory of Cell Culture, Centro Universitário Franciscano, Santa Maria, RS, Brazil

700    1_

$a Ramos, Andiara P. $u Laboratory of Cell Culture, Centro Universitário Franciscano, Santa Maria, RS, Brazil

700    1_

$a Sagrillo, Michele R. $u Laboratory of Cell Culture, Centro Universitário Franciscano, Santa Maria, RS, Brazil

700    1_

$a Vaucher, Rodrigo A. $u Laboratory of Cell Culture, Centro Universitário Franciscano, Santa Maria, RS, Brazil

700    1_

$a Da Silva, Aleksandro S. $u Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil

700    1_

$a Becker, Ana P. $u Laboratory of Cell Culture, Centro Universitário Franciscano, Santa Maria, RS, Brazil

700    1_

$a Monteiro, Silvia G. $u Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazi

773    0_

$t Journal of applied biomedicine $x 1214-021X $g Roč. 14, č. 4 (2016), s. 265-272 $w MED00012667

856    41

$u https://jab.zsf.jcu.cz/pdfs/jab/2016/04/03.pdf $y plný text volně přístupný

910    __

$a ABA008 $b B 2301 $c 1249 $y 4 $z 0

990    __

$a 20170407165434 $b ABA008

991    __

$a 20170531103333 $b ABA008

999    __

$a ok $b bmc $g 1198021 $s 972921

BAS    __

$a 3

BMC    __

$a 2016 $b 14 $c 4 $d 265-272 $i 1214-021X $m Journal of Applied Biomedicine $x MED00012667

LZP    __

$c NLK125 $d 20170531 $a NLK 2017-15/dk