The aim of this study was to investigate the occurrence of toxic effects in liver tissue of rats treated with α-terpinene. All treatments were intraperitoneally administered at doses of 0.5, 0.75 and 1.0 ml kg−1 during 10 days. Liver samples were collected and assessed by histopathological analysis, caspases -1, -3, -8 assay, biomarkers of hepatic damage and determination of oxidant/antioxidant status (thiobarbituric acid-reactive substances (TBARS), catalase (CAT), superoxide dismutase (SOD), reactive oxygen species (ROS), glutathione S-transferase (GST) and glutathione peroxidase (GPx)). Additionally, the cytotoxic and genotoxic effects were evaluated by comet assay. An increase was observed on TBARS levels and GPx activity on the hepatic tissue. Instead, CAT and SOD activities decreased in rats treated with a dose of 1.0 ml kg−1 of α-terpinene. Concomitantly, ROS levels increased and GST levels decreased in rats treated with α-terpinene at doses of 0.5, 0.75 and 1.0 ml kg−1. Also, there was an increase in frequency of damage, damage index and caspases, while cell viability decreased in rats treated with α-terpinene. Alanine aminotransferase and aspartate aminotransferase increased in rats treated with 1.0 ml kg−1 of α-terpinene. Therefore, α-terpinene induces oxidative stress, cytotoxic and genotoxic effects in liver tissue involving the caspases activation.
- MeSH
- apoptóza účinky léků MeSH
- glutathiontransferasa účinky léků MeSH
- imunoanalýza MeSH
- játra * patologie účinky léků MeSH
- kaspasy * MeSH
- kometový test MeSH
- krysa rodu rattus MeSH
- monoterpeny * aplikace a dávkování toxicita MeSH
- oxidační stres MeSH
- poškození DNA účinky léků MeSH
- potkani Wistar MeSH
- reaktivní formy kyslíku analýza MeSH
- testy genotoxicity MeSH
- viabilita buněk účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
The effect of the combination of α-pinene and β-caryophyllene on the proliferation of the Trypanosoma evansi was analyzed. These compounds were added individually and in combination at different concentrations to T. evansi cultures, and the in vivo study used mice of 1.0 and 1.5 mL kg−1 of α-pinene and β-caryophyllene individually and in combination against T. evansi. In order to evaluate the in vitro safety, lymphocytes and VERO cells were assessed using tetrazolium salt (MTT) assay. The trypanocidal effect of α-pinene and β-caryophyllene was observed in vitro when applied independently or combined at 0.5, 1 and 2% concentrations. The combination effect showed a faster trypanocidal effect when compared to chemotherapy (diminazene aceturate – D.A.). Concerning the in vivo evaluation, α-pinene + β-caryophyllene at 1.0 mL kg−1 was able to extend animal longevity and showed 83.33% curative efficacy, a superior result when compared to D.A. (16.66%). The MTT assay showed that the assessed compounds do not present toxicity to used cells. Also, in vivo safety evaluation of α-pinene + β-caryophyllene at 1.0 mL kg−1 not altered the aminotransferases levels in serum. Combination between α-pinene and β-caryophyllene shows in vitro and in vivo trypanocidal activity against T. evansi, in a safe dose.
- MeSH
- modely u zvířat MeSH
- monoterpeny aplikace a dávkování farmakologie chemie MeSH
- myši MeSH
- seskviterpeny aplikace a dávkování farmakologie chemie MeSH
- trypanocidální látky aplikace a dávkování MeSH
- Trypanosoma izolace a purifikace účinky léků MeSH
- vztahy mezi strukturou a aktivitou MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Klíčová slova
- cholagol,
- MeSH
- adherence k farmakoterapii MeSH
- fixní kombinace léků MeSH
- hodnocení léčiv MeSH
- kontrolní skupiny MeSH
- lidé MeSH
- monoterpeny * aplikace a dávkování farmakokinetika škodlivé účinky terapeutické užití MeSH
- nemoci žlučového ústrojí * farmakoterapie MeSH
- oleje prchavé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- klinická studie MeSH
- tabulky MeSH
