Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Plasma concentrations and subcutaneous adipose tissue mRNA expression of clusterin in obesity and type 2 diabetes mellitus: the effect of short-term hyperinsulinemia, very-low-calorie diet and bariatric surgery

J. Kloučková, Z. Lacinová, P. Kaválková, P. Trachta, M. Kasalický, D. Haluzíková, M. Mráz, M. Haluzík

. 2016 ; 65 (3) : 481-492. [pub] 20160412

Language English Country Czech Republic

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc17012736

Grant support
NT13299 MZ0 CEP Register

Clusterin is a heterodimeric glycoprotein with wide range of functions. To further explore its possible regulatory role in energy homeostasis and in adipose tissue, we measured plasma clusterin and its mRNA expression in subcutaneous adipose tissue (SCAT) of 15 healthy lean women, 15 obese women (OB) and 15 obese women with type 2 diabetes mellitus (T2DM) who underwent a 2-week very low-calorie diet (VLCD), 10 obese women without T2DM who underwent laparoscopic sleeve gastrectomy (LSG) and 8 patients with T2DM, 8 patients with impaired glucose tolerance (IGT) and 8 normoglycemic patients who underwent hyperinsulinemic euglycemic clamp (HEC). VLCD decreased plasma clusterin in OB but not in T2DM patients while LSG and HEC had no effect. Clusterin mRNA expression in SCAT at baseline was increased in OB and T2DM patients compared with controls. Clusterin mRNA expression decreased 6 months after LSG and remained decreased 12 months after LSG. mRNA expression of clusterin was elevated at the end of HEC compared with baseline only in normoglycemic but not in IGT or T2DM patients. In summary, our data suggest a possible local regulatory role for clusterin in the adipose tissue rather than its systemic involvement in the regulation of energy homeostasis.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17012736
003      
CZ-PrNML
005      
20190827094500.0
007      
ta
008      
170412s2016 xr a f 000 0|eng||
009      
AR
024    7_
$a 10.33549/physiolres.933121 $2 doi
035    __
$a (PubMed)27070750
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Kloučková, Jana. $u Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic $7 xx0232313
245    10
$a Plasma concentrations and subcutaneous adipose tissue mRNA expression of clusterin in obesity and type 2 diabetes mellitus: the effect of short-term hyperinsulinemia, very-low-calorie diet and bariatric surgery / $c J. Kloučková, Z. Lacinová, P. Kaválková, P. Trachta, M. Kasalický, D. Haluzíková, M. Mráz, M. Haluzík
520    9_
$a Clusterin is a heterodimeric glycoprotein with wide range of functions. To further explore its possible regulatory role in energy homeostasis and in adipose tissue, we measured plasma clusterin and its mRNA expression in subcutaneous adipose tissue (SCAT) of 15 healthy lean women, 15 obese women (OB) and 15 obese women with type 2 diabetes mellitus (T2DM) who underwent a 2-week very low-calorie diet (VLCD), 10 obese women without T2DM who underwent laparoscopic sleeve gastrectomy (LSG) and 8 patients with T2DM, 8 patients with impaired glucose tolerance (IGT) and 8 normoglycemic patients who underwent hyperinsulinemic euglycemic clamp (HEC). VLCD decreased plasma clusterin in OB but not in T2DM patients while LSG and HEC had no effect. Clusterin mRNA expression in SCAT at baseline was increased in OB and T2DM patients compared with controls. Clusterin mRNA expression decreased 6 months after LSG and remained decreased 12 months after LSG. mRNA expression of clusterin was elevated at the end of HEC compared with baseline only in normoglycemic but not in IGT or T2DM patients. In summary, our data suggest a possible local regulatory role for clusterin in the adipose tissue rather than its systemic involvement in the regulation of energy homeostasis.
650    12
$a bariatrická chirurgie $7 D050110
650    12
$a kalorická restrikce $7 D031204
650    _2
$a studie případů a kontrol $7 D016022
650    _2
$a klusterin $x krev $7 D051152
650    _2
$a diabetes mellitus 2. typu $x krev $x komplikace $7 D003924
650    _2
$a energetický metabolismus $7 D004734
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a lidé $7 D006801
650    _2
$a lidé středního věku $7 D008875
650    _2
$a obezita $x krev $x komplikace $x terapie $7 D009765
650    _2
$a čipová analýza proteinů $7 D040081
650    _2
$a messenger RNA $x metabolismus $7 D012333
650    _2
$a podkožní tuk $x metabolismus $7 D050151
655    _2
$a časopisecké články $7 D016428
700    1_
$a Lacinová, Zdeňka $7 xx0062138 $u Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic; Third Department of Medicine, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic
700    1_
$a Kaválková, Petra $7 xx0094376 $u Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic
700    1_
$a Trachta, Pavel $7 xx0248280 $u Third Department of Medicine, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic
700    1_
$a Kasalický, Mojmír, $d 1959- $7 mzk2007395028 $u Department of Surgery, Second Faculty of Medicine, Charles University and Military University Hospital, Prague, Czech Republic
700    1_
$a Haluzíková, Denisa, $d 1970- $7 jo2003163140 $u Department of Sports Medicine, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic
700    1_
$a Mráz, Miloš $7 xx0119173 $u Third Department of Medicine, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic
700    1_
$a Haluzík, Martin, $d 1970- $7 xx0000707 $u Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic; Institute of Endocrinology, Prague, Czech Republic
773    0_
$w MED00003824 $t Physiological research $x 1802-9973 $g Roč. 65, č. 3 (2016), s. 481-492
856    41
$u http://www.biomed.cas.cz/physiolres/ $y domovská stránka časopisu
910    __
$a ABA008 $b A 4120 $c 266 $y 4 $z 0
990    __
$a 20170412 $b ABA008
991    __
$a 20190827094720 $b ABA008
999    __
$a ok $b bmc $g 1201671 $s 973509
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2016 $b 65 $c 3 $d 481-492 $e 20160412 $i 1802-9973 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
GRA    __
$a NT13299 $p MZ0
LZP    __
$b NLK118 $a Pubmed-20170412
Back

Find record

Citation metrics

Logged in users only

Archiving options