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The crucial role of emilin 1 gene expression during progression of tumor growth
M. Rabajdova, P. Urban, I. Spakova, L. Saksun, R. Dudic, A. Ostro, M. Caprnda, P. Kruzliak, M. Adamek, M. Marekova,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
NLK
ProQuest Central
od 1997-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2003-04-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 1997-01-01 do Před 1 rokem
Public Health Database (ProQuest)
od 1997-01-01 do Před 1 rokem
Springer Nature OA/Free Journals
od 1904-02-01
- MeSH
- duktální karcinom prsu genetika metabolismus MeSH
- exprese genu MeSH
- genová dávka MeSH
- komplementární DNA genetika metabolismus MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lidé MeSH
- membránové glykoproteiny biosyntéza genetika MeSH
- messenger RNA genetika metabolismus MeSH
- nádory prsu genetika metabolismus MeSH
- studie případů a kontrol MeSH
- stupeň nádoru MeSH
- western blotting MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: This study describes the effect of rapid tumor growth of patients suffering from various grades of malignant ductal breast carcinoma associated with the gene expression of ECM protein emilin 1, in correlation with the number of gene copies of emilin 1 and degradation of tumor tissue proteins. METHODS: A total of 40 examined patients participated in the experiment (controls, n = 10, grades GI-GIII, each n = 10). After isolation of total mRNA, transcription of mRNA into the cDNA was performed. Quantification of gene expression changes was detected by the real-time PCR method. Analysis at the protein level was performed via Western blot method. RESULTS: During the detection of changes at the mRNA level, a significantly decreased level of emilin 1 in tumor tissues with grade II (about 54 ± 8 % lower than control) was identified. Protein-level analysis indicated an increased level of emilin 1 in tumors with grade I in comparison with control samples (about 10 ± 3 %). CONCLUSION: Obtained results demonstrated that the suppressive role of emilin 1 is related to the grade of growing breast tumors, and associated with increased hypoxia in the tumor microenvironment followed by elevated unfolding and degradation of tissue proteins.
2nd Department of Internal Medicine Faculty of Medicine Comenius University Bratislava Slovakia
Department of Pathology Saca Hospital Kosice Slovakia
Department of Thoracic Surgery Medical University of Silesia Zabrze Poland
Citace poskytuje Crossref.org
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