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Gene Expression Profiling in Fish Toxicology: A Review
G. Kumar, ND. Denslow,
Language English Country United States
Document type Journal Article, Review
PubMed
27464848
DOI
10.1007/398_2016_10
Knihovny.cz E-resources
- MeSH
- Water Pollutants, Chemical toxicity MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Fishes genetics MeSH
- Gene Expression Profiling * MeSH
- Subtractive Hybridization Techniques MeSH
- Toxicology methods MeSH
- High-Throughput Nucleotide Sequencing MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
In this review, we present an overview of transcriptomic responses to chemical exposures in a variety of fish species. We have discussed the use of several molecular approaches such as northern blotting, differential display reverse transcription-polymerase chain reaction (DDRT-PCR), suppression subtractive hybridization (SSH), real time quantitative PCR (RT-qPCR), microarrays, and next-generation sequencing (NGS) for measuring gene expression. These techniques have been mainly used to measure the toxic effects of single compounds or simple mixtures in laboratory conditions. In addition, only few studies have been conducted to examine the biological significance of differentially expressed gene sets following chemical exposure. Therefore, future studies should focus more under field conditions using a multidisciplinary approach (genomics, proteomics and metabolomics) to understand the synergetic effects of multiple environmental stressors and to determine the functional significance of differentially expressed genes. Nevertheless, recent developments in NGS technologies and decreasing costs of sequencing holds the promise to uncover the complexity of anthropogenic impacts and biological effects in wild fish populations.
References provided by Crossref.org
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- $a Kumar, Girish $u Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic. girishkumar.nio@gmail.com.
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