-
Je něco špatně v tomto záznamu ?
Human Multipotent Mesenchymal Stromal Cells in the Treatment of Postoperative Temporal Bone Defect: An Animal Model
L. Skoloudik, V. Chrobok, D. Kalfert, Z. Koci, E. Sykova, T. Chumak, J. Popelar, J. Syka, J. Laco, J. Dedková, G. Dayanithi, S. Filip,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
ProQuest Central
od 2016-01-01
Health & Medicine (ProQuest)
od 2016-01-01
ROAD: Directory of Open Access Scholarly Resources
od 1992
PubMed
26497735
DOI
10.3727/096368915x689730
Knihovny.cz E-zdroje
- MeSH
- biologické markery metabolismus MeSH
- lidé MeSH
- mezenchymální kmenové buňky cytologie MeSH
- modely nemocí na zvířatech MeSH
- morčata MeSH
- multipotentní kmenové buňky cytologie MeSH
- počítačová rentgenová tomografie MeSH
- pooperační péče * MeSH
- spánková kost diagnostické zobrazování patologie chirurgie MeSH
- transplantace mezenchymálních kmenových buněk * MeSH
- vnitřní ucho patologie MeSH
- zánět patologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- morčata MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Canal wall down mastoidectomy is one of the most effective treatments for cholesteatoma. However, it results in anatomical changes in the external and middle ear with a negative impact on the patient's quality of life. To provide complete closure of the mastoid cavity and normalize the anatomy of the middle and external ear, we used human multipotent mesenchymal stromal cells (hMSCs), GMP grade, in a guinea pig model. A method for preparing a biomaterial composed of hMSCs, hydroxyapatite, and tissue glue was developed. Animals from the treated group were implanted with biomaterial composed of hydroxyapatite and hMSCs, while animals in the control group received hydroxyapatite alone. When compared to controls, the group implanted with hMSCs showed a significantly higher ratio of new bone formation (p = 0.00174), as well as a significantly higher volume percentage of new immature bone (p = 0.00166). Our results proved a beneficial effect of hMSCs on temporal bone formation and provided a promising tool to improve the quality of life of patients after canal wall down mastoidectomy by hMSC implantation.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc17014236
- 003
- CZ-PrNML
- 005
- 20170427111145.0
- 007
- ta
- 008
- 170413s2016 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.3727/096368915X689730 $2 doi
- 035 __
- $a (PubMed)26497735
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Skoloudik, Lukas $u Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital Hradec Kralové, Charles University in Prague, Faculty of Medicine in Hradec Kralové, Hradec Kralové, Czech Republic.
- 245 10
- $a Human Multipotent Mesenchymal Stromal Cells in the Treatment of Postoperative Temporal Bone Defect: An Animal Model / $c L. Skoloudik, V. Chrobok, D. Kalfert, Z. Koci, E. Sykova, T. Chumak, J. Popelar, J. Syka, J. Laco, J. Dedková, G. Dayanithi, S. Filip,
- 520 9_
- $a Canal wall down mastoidectomy is one of the most effective treatments for cholesteatoma. However, it results in anatomical changes in the external and middle ear with a negative impact on the patient's quality of life. To provide complete closure of the mastoid cavity and normalize the anatomy of the middle and external ear, we used human multipotent mesenchymal stromal cells (hMSCs), GMP grade, in a guinea pig model. A method for preparing a biomaterial composed of hMSCs, hydroxyapatite, and tissue glue was developed. Animals from the treated group were implanted with biomaterial composed of hydroxyapatite and hMSCs, while animals in the control group received hydroxyapatite alone. When compared to controls, the group implanted with hMSCs showed a significantly higher ratio of new bone formation (p = 0.00174), as well as a significantly higher volume percentage of new immature bone (p = 0.00166). Our results proved a beneficial effect of hMSCs on temporal bone formation and provided a promising tool to improve the quality of life of patients after canal wall down mastoidectomy by hMSC implantation.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a biologické markery $x metabolismus $7 D015415
- 650 _2
- $a modely nemocí na zvířatech $7 D004195
- 650 _2
- $a vnitřní ucho $x patologie $7 D007758
- 650 _2
- $a morčata $7 D006168
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a zánět $x patologie $7 D007249
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 12
- $a transplantace mezenchymálních kmenových buněk $7 D045164
- 650 _2
- $a mezenchymální kmenové buňky $x cytologie $7 D059630
- 650 _2
- $a multipotentní kmenové buňky $x cytologie $7 D039902
- 650 12
- $a pooperační péče $7 D011182
- 650 _2
- $a spánková kost $x diagnostické zobrazování $x patologie $x chirurgie $7 D013701
- 650 _2
- $a počítačová rentgenová tomografie $7 D014057
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Chrobok, Viktor
- 700 1_
- $a Kalfert, David
- 700 1_
- $a Koci, Zuzana
- 700 1_
- $a Sykova, Eva
- 700 1_
- $a Chumak, Tetyana
- 700 1_
- $a Popelar, Jiri
- 700 1_
- $a Syka, Josef
- 700 1_
- $a Laco, Jan
- 700 1_
- $a Dedková, Jana
- 700 1_
- $a Dayanithi, Govindan
- 700 1_
- $a Filip, Stanislav
- 773 0_
- $w MED00001075 $t Cell transplantation $x 1555-3892 $g Roč. 25, č. 7 (2016), s. 1405-14
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/26497735 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20170413 $b ABA008
- 991 __
- $a 20170427111505 $b ABA008
- 999 __
- $a ok $b bmc $g 1200701 $s 975014
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2016 $b 25 $c 7 $d 1405-14 $e 20151022 $i 1555-3892 $m Cell transplantation $n Cell Transplant $x MED00001075
- LZP __
- $a Pubmed-20170413
