-
Je něco špatně v tomto záznamu ?
Droplet digital PCR analysis of GSTM1 deletion polymorphism in psoriatic subjects treated with goeckerman therapy
M. Beránek, Z. Fiala, J. Kremláček, C. Andrýs, K. Hamáková, V. Palička, L. Borská
Jazyk angličtina Země Česko
Typ dokumentu časopisecké články
Digitální knihovna NLK
Plný text - Článek
Číslo
Ročník
Zdroj
Zdroj
NLK
Directory of Open Access Journals
od 1997
Free Medical Journals
od 1997
Open Access Digital Library
od 1997-01-01
Medline Complete (EBSCOhost)
od 2012-06-01
ROAD: Directory of Open Access Scholarly Resources
od 1997
- MeSH
- adukty DNA MeSH
- dehet uhelný terapeutické užití MeSH
- dospělí MeSH
- genotyp MeSH
- glutathiontransferasa genetika MeSH
- keratolytika terapeutické užití MeSH
- kombinovaná terapie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mutační analýza DNA metody MeSH
- polymerázová řetězová reakce metody MeSH
- polymorfismus genetický MeSH
- psoriáza genetika terapie MeSH
- sekvence nukleotidů * MeSH
- sekvenční delece * MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- terapie ultrafialovými paprsky MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Goeckerman therapy (GT) represents an effective treatment of psoriasis including a combination of pharmaceutical grade crude coal tar (CCT) and ultraviolet irradiation (UV-R). Coal tar contains a mixture of polycyclic aromatic hydrocarbons. The best known carcinogenic polyaromate - benzo[a]pyrene is metabolized into a highly reactive benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE). Glutathione S-transferase M1 (GSTM1) catalyses the conjugation of drugs, toxins and products of oxidative stress with glutathione. The aim of the study is to found possible associations between GSTM1 genotypes and the level of BPDE-DNA adducts in 46 psoriatic patients treated with GT. For genotyping, droplet digital PCR was applied. The GSTM1 copy number was normalized to β-globin reference gene. In five GSTM1*1/*1 subjects, the GSTM1 to β-globin ratio moved from 0.99 to 1.03 with a median of 1.01. GSTM1*0/*1 heterozygotes (n = 20) contained only one GSTM1 function allele which conditioned the ratio 0.47-0.53 (median 0.50). GSTM1*0/*0 individuals (n = 21) showed no amplification of the null variants because of the large deletion in GSTM1. BPDE-DNA concentrations ranged from 1.8 to 66.3 ng/µg with a median of 12.3 ng/µg. GSTM1*0/*0 and GSTM1*0/*1 genotypes showed non-significantly higher concentrations of BPDE-DNA adducts than the GSTM1*1/*1 one (12.3 and 12.4 vs 7.8 ng/µg). The non-significant relationship between BPDE-DNA adducts and GSTM1 genotypes in psoriatic patients could be associated with relatively low doses of CCT and short-term UV-R exposures used in GT.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc17020882
- 003
- CZ-PrNML
- 005
- 20170704134524.0
- 007
- ta
- 008
- 170623s2016 xr d f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.14712/18059694.2016.94 $2 doi
- 035 __
- $a (PubMed)27639151
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Beránek, Martin, $u Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Hradec Králové, Czech Republic; Institute of Clinical Biochemistry and Diagnostics, Charles University Hospital and Faculty of Medicine in Hradec Králové, Hradec Králové, Czech Republic $d 1968- $7 xx0044204
- 245 10
- $a Droplet digital PCR analysis of GSTM1 deletion polymorphism in psoriatic subjects treated with goeckerman therapy / $c M. Beránek, Z. Fiala, J. Kremláček, C. Andrýs, K. Hamáková, V. Palička, L. Borská
- 520 9_
- $a Goeckerman therapy (GT) represents an effective treatment of psoriasis including a combination of pharmaceutical grade crude coal tar (CCT) and ultraviolet irradiation (UV-R). Coal tar contains a mixture of polycyclic aromatic hydrocarbons. The best known carcinogenic polyaromate - benzo[a]pyrene is metabolized into a highly reactive benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE). Glutathione S-transferase M1 (GSTM1) catalyses the conjugation of drugs, toxins and products of oxidative stress with glutathione. The aim of the study is to found possible associations between GSTM1 genotypes and the level of BPDE-DNA adducts in 46 psoriatic patients treated with GT. For genotyping, droplet digital PCR was applied. The GSTM1 copy number was normalized to β-globin reference gene. In five GSTM1*1/*1 subjects, the GSTM1 to β-globin ratio moved from 0.99 to 1.03 with a median of 1.01. GSTM1*0/*1 heterozygotes (n = 20) contained only one GSTM1 function allele which conditioned the ratio 0.47-0.53 (median 0.50). GSTM1*0/*0 individuals (n = 21) showed no amplification of the null variants because of the large deletion in GSTM1. BPDE-DNA concentrations ranged from 1.8 to 66.3 ng/µg with a median of 12.3 ng/µg. GSTM1*0/*0 and GSTM1*0/*1 genotypes showed non-significantly higher concentrations of BPDE-DNA adducts than the GSTM1*1/*1 one (12.3 and 12.4 vs 7.8 ng/µg). The non-significant relationship between BPDE-DNA adducts and GSTM1 genotypes in psoriatic patients could be associated with relatively low doses of CCT and short-term UV-R exposures used in GT.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a senioři $7 D000368
- 650 _2
- $a senioři nad 80 let $7 D000369
- 650 12
- $a sekvence nukleotidů $7 D001483
- 650 _2
- $a dehet uhelný $x terapeutické užití $7 D003033
- 650 _2
- $a kombinovaná terapie $7 D003131
- 650 _2
- $a adukty DNA $7 D018736
- 650 _2
- $a mutační analýza DNA $x metody $7 D004252
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a genotyp $7 D005838
- 650 _2
- $a glutathiontransferasa $x genetika $7 D005982
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a keratolytika $x terapeutické užití $7 D007641
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a polymerázová řetězová reakce $x metody $7 D016133
- 650 _2
- $a polymorfismus genetický $7 D011110
- 650 _2
- $a psoriáza $x genetika $x terapie $7 D011565
- 650 12
- $a sekvenční delece $7 D017384
- 650 _2
- $a terapie ultrafialovými paprsky $7 D014467
- 650 _2
- $a mladý dospělý $7 D055815
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Fiala, Zdeněk, $u Institute of Hygiene and Preventive Medicine, Charles University in Prague, Faculty of Medicine in Hradec Králové, Hradec Králové, Czech Republic $d 1955- $7 nlk19990073147
- 700 1_
- $a Kremláček, Jan, $u Institute of Pathological Physiology, Charles University in Prague, Faculty of Medicine in Hradec Králové, Hradec Králové, Czech Republic $d 1967- $7 mzk2003171485
- 700 1_
- $a Andrýs, Ctirad, $u Institute of Clinical Immunology and Allergology, Charles University in Prague, Faculty of Medicine in Hradec Králové, Hradec Králové, Czech Republic $d 1962- $7 mzk2008430528
- 700 1_
- $a Hamáková, Květoslava $u Clinic of Dermal and Venereal Diseases, Charles University Hospital Hradec Králové, Hradec Králové, Czech Republic $7 xx0064204
- 700 1_
- $a Palička, Vladimír, $u Institute of Clinical Biochemistry and Diagnostics, Charles University Hospital and Faculty of Medicine in Hradec Králové, Hradec Králové, Czech Republic $d 1946- $7 jn99240000830
- 700 1_
- $a Borská, Lenka $u Institute of Pathological Physiology, Charles University in Prague, Faculty of Medicine in Hradec Králové, Hradec Králové, Czech Republic $7 nlk20060173900
- 773 0_
- $w MED00010947 $t Acta Medica (Hradec Kralove) $x 1211-4286 $g Roč. 59, č. 3 (2016), s. 75-78
- 856 41
- $u https://actamedica.lfhk.cuni.cz/media/pdf/18059694.2016.94.pdf $y plný text volně přístupný
- 910 __
- $a ABA008 $b A 3077 $c 1072 $y 4 $z 0
- 990 __
- $a 20170623 $b ABA008
- 991 __
- $a 20170629141211 $b ABA008
- 999 __
- $a ok $b bmc $g 1236245 $s 981755
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2016 $b 59 $c 3 $d 75-78 $e 20160829 $i 1211-4286 $m Acta Medica $n Acta Med. (Hradec Král.) $x MED00010947
- LZP __
- $b NLK118 $a Pubmed-20170623
