Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

From the Tunnels into the Treetops: New Lineages of Black Yeasts from Biofilm in the Stockholm Metro System and Their Relatives among Ant-Associated Fungi in the Chaetothyriales

M. Réblová, V. Hubka, O. Thureborn, J. Lundberg, T. Sallstedt, M. Wedin, M. Ivarsson,

. 2016 ; 11 (10) : e0163396. [pub] 20161012

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc17023641

Rock-inhabiting fungi harbour species-rich, poorly differentiated, extremophilic taxa of polyphyletic origin. Their closest relatives are often well-known species from various biotopes with significant pathogenic potential. Speleothems represent a unique rock-dwelling habitat, whose mycobiota are largely unexplored. Isolation of fungi from speleothem biofilm covering bare granite walls in the Kungsträdgården metro station in Stockholm yielded axenic cultures of two distinct black yeast morphotypes. Phylogenetic analyses of DNA sequences from six nuclear loci, ITS, nuc18S and nuc28S rDNA, rpb1, rpb2 and β-tubulin, support their placement in the Chaetothyriales (Ascomycota). They are described as a new genus Bacillicladium with the type species B. lobatum, and a new species Bradymyces graniticola. Bacillicladium is distantly related to the known five chaetothyrialean families and is unique in the Chaetothyriales by variable morphology showing hyphal, meristematic and yeast-like growth in vitro. The nearest relatives of Bacillicladium are recruited among fungi isolated from cardboard-like construction material produced by arboricolous non-attine ants. Their sister relationship is weakly supported by the Maximum likelihood analysis, but strongly supported by Bayesian inference. The genus Bradymyces is placed amidst members of the Trichomeriaceae and is ecologically undefined; it includes an opportunistic animal pathogen while two other species inhabit rock surfaces. ITS rDNA sequences of three species accepted in Bradymyces and other undescribed species and environmental samples were subjected to phylogenetic analysis and in-depth comparative analysis of ITS1 and ITS2 secondary structures in order to study their intraspecific variability. Compensatory base change criterion in the ITS2 secondary structure supported delimitation of species in Bradymyces, which manifest a limited number of phenotypic features useful for species recognition. The role of fungi in the speleothem biofilm and relationships of Bacillicladium and Bradymyces with other members of the Chaetothyriales are discussed.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17023641
003      
CZ-PrNML
005      
20170720122308.0
007      
ta
008      
170720s2016 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1371/journal.pone.0163396 $2 doi
035    __
$a (PubMed)27732675
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Réblová, Martina $u Department of Taxonomy, Institute of Botany of the Czech Academy of Sciences, 252 43, Průhonice, Czech Republic.
245    10
$a From the Tunnels into the Treetops: New Lineages of Black Yeasts from Biofilm in the Stockholm Metro System and Their Relatives among Ant-Associated Fungi in the Chaetothyriales / $c M. Réblová, V. Hubka, O. Thureborn, J. Lundberg, T. Sallstedt, M. Wedin, M. Ivarsson,
520    9_
$a Rock-inhabiting fungi harbour species-rich, poorly differentiated, extremophilic taxa of polyphyletic origin. Their closest relatives are often well-known species from various biotopes with significant pathogenic potential. Speleothems represent a unique rock-dwelling habitat, whose mycobiota are largely unexplored. Isolation of fungi from speleothem biofilm covering bare granite walls in the Kungsträdgården metro station in Stockholm yielded axenic cultures of two distinct black yeast morphotypes. Phylogenetic analyses of DNA sequences from six nuclear loci, ITS, nuc18S and nuc28S rDNA, rpb1, rpb2 and β-tubulin, support their placement in the Chaetothyriales (Ascomycota). They are described as a new genus Bacillicladium with the type species B. lobatum, and a new species Bradymyces graniticola. Bacillicladium is distantly related to the known five chaetothyrialean families and is unique in the Chaetothyriales by variable morphology showing hyphal, meristematic and yeast-like growth in vitro. The nearest relatives of Bacillicladium are recruited among fungi isolated from cardboard-like construction material produced by arboricolous non-attine ants. Their sister relationship is weakly supported by the Maximum likelihood analysis, but strongly supported by Bayesian inference. The genus Bradymyces is placed amidst members of the Trichomeriaceae and is ecologically undefined; it includes an opportunistic animal pathogen while two other species inhabit rock surfaces. ITS rDNA sequences of three species accepted in Bradymyces and other undescribed species and environmental samples were subjected to phylogenetic analysis and in-depth comparative analysis of ITS1 and ITS2 secondary structures in order to study their intraspecific variability. Compensatory base change criterion in the ITS2 secondary structure supported delimitation of species in Bradymyces, which manifest a limited number of phenotypic features useful for species recognition. The role of fungi in the speleothem biofilm and relationships of Bacillicladium and Bradymyces with other members of the Chaetothyriales are discussed.
650    _2
$a zvířata $7 D000818
650    _2
$a Formicidae $x mikrobiologie $7 D001000
650    _2
$a Ascomycota $x klasifikace $x genetika $x fyziologie $7 D001203
650    _2
$a sekvence nukleotidů $7 D001483
650    _2
$a Bayesova věta $7 D001499
650    _2
$a biofilmy $7 D018441
650    _2
$a DNA fungální $x chemie $x izolace a purifikace $x metabolismus $7 D004271
650    _2
$a ribozomální DNA $x chemie $x izolace a purifikace $x metabolismus $7 D004275
650    _2
$a fungální proteiny $x genetika $7 D005656
650    _2
$a konformace nukleové kyseliny $7 D009690
650    _2
$a fylogeneze $7 D010802
650    _2
$a RNA-polymerasa II $x genetika $7 D012319
650    _2
$a sekvenční seřazení $7 D016415
650    _2
$a Švédsko $7 D013548
650    _2
$a tubulin $x genetika $7 D014404
655    _2
$a časopisecké články $7 D016428
700    1_
$a Hubka, Vit $u Department of Botany, Faculty of Science, Charles University in Prague, 128 01, Prague, 2, Czech Republic. Laboratory of Fungal Genetics and Metabolism, Institute of Microbiology of the Czech Academy of Sciences, 142 20, Prague, 4, Czech Republic.
700    1_
$a Thureborn, Olle $u Department of Ecology, Environment and Plant Sciences, Stockholm University, 106 91, Stockholm, Sweden.
700    1_
$a Lundberg, Johannes $u Department of Botany, Swedish Museum of Natural History, 104 05, Stockholm, Sweden.
700    1_
$a Sallstedt, Therese $u Department of Biology, University of Southern Denmark, 5230, Odense, Denmark. Department of Palaeobiology, Swedish Museum of Natural History, 104 05, Stockholm, Sweden.
700    1_
$a Wedin, Mats $u Department of Botany, Swedish Museum of Natural History, 104 05, Stockholm, Sweden.
700    1_
$a Ivarsson, Magnus $u Department of Palaeobiology, Swedish Museum of Natural History, 104 05, Stockholm, Sweden.
773    0_
$w MED00180950 $t PloS one $x 1932-6203 $g Roč. 11, č. 10 (2016), s. e0163396
856    41
$u https://pubmed.ncbi.nlm.nih.gov/27732675 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20170720 $b ABA008
991    __
$a 20170720122801 $b ABA008
999    __
$a ok $b bmc $g 1239322 $s 984554
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2016 $b 11 $c 10 $d e0163396 $e 20161012 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
LZP    __
$a Pubmed-20170720

Najít záznam

Citační ukazatele

Možnosti archivace