Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Efficacy and safety of human mesenchymal stromal cells in healing of critical-size bone defects in immunodeficient rats

R. Pytlík, C. Rentsch, T. Soukup, L. Novotný, B. Rentsch, V. Kanderová, H. Rychtrmocová, M. Kalmárová, D. Stehlík, M. Trněný, O. Slanař

. 2017 ; 66 (1) : 113-123. [pub] 20161026

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc17026019

Grantová podpora
NT13531 MZ0 CEP - Centrální evidence projektů

To evaluate the preclinical efficacy and safety of human mesenchymal stem cells (hMSC) rapidly expanded in growth medium for clinical use with human serum and recombinant growth factors, we conducted a controlled, randomized trial of plasma clots with hMSC vs. plasma clots only in critical segmental femoral defects in rnu/rnu immunodeficient rats. X-ray, microCT and histomorphometrical evaluation were performed at 8 and 16 weeks. MSC were obtained from healthy volunteers and patients with lymphoid malignancy. Human MSC survived in the defect for the entire duration of the trial. MSC from healthy volunteers, in contrast to hMSC from cancer patients, significantly improved bone healing at 8, but not 16 weeks. However, at 16 weeks, hMSC significantly improved vasculogenesis in residual defect. We conclude that hMSC from healthy donors significantly contributed to the healing of bone defects at 8 weeks and to the vascularisation of residual connective tissue for up to 16 weeks. We found the administration of hMSC to be safe, as no adverse reaction to human cells at the site of implantation and no evidence of migration of hMSC to distant organs was detected.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17026019
003      
CZ-PrNML
005      
20190514105832.0
007      
ta
008      
170824s2017 xr ad f 000 0|eng||
009      
AR
024    7_
$a 10.33549/physiolres.933376 $2 doi
035    __
$a (PubMed)27782744
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Pytlík, Robert, $u First Department of Medicine, First Medical Faculty, Charles University, Prague and General University Hospital, Prague, Czech Republic $d 1967- $7 xx0061345
245    10
$a Efficacy and safety of human mesenchymal stromal cells in healing of critical-size bone defects in immunodeficient rats / $c R. Pytlík, C. Rentsch, T. Soukup, L. Novotný, B. Rentsch, V. Kanderová, H. Rychtrmocová, M. Kalmárová, D. Stehlík, M. Trněný, O. Slanař
520    9_
$a To evaluate the preclinical efficacy and safety of human mesenchymal stem cells (hMSC) rapidly expanded in growth medium for clinical use with human serum and recombinant growth factors, we conducted a controlled, randomized trial of plasma clots with hMSC vs. plasma clots only in critical segmental femoral defects in rnu/rnu immunodeficient rats. X-ray, microCT and histomorphometrical evaluation were performed at 8 and 16 weeks. MSC were obtained from healthy volunteers and patients with lymphoid malignancy. Human MSC survived in the defect for the entire duration of the trial. MSC from healthy volunteers, in contrast to hMSC from cancer patients, significantly improved bone healing at 8, but not 16 weeks. However, at 16 weeks, hMSC significantly improved vasculogenesis in residual defect. We conclude that hMSC from healthy donors significantly contributed to the healing of bone defects at 8 weeks and to the vascularisation of residual connective tissue for up to 16 weeks. We found the administration of hMSC to be safe, as no adverse reaction to human cells at the site of implantation and no evidence of migration of hMSC to distant organs was detected.
650    _2
$a dospělí $7 D000328
650    _2
$a senioři $7 D000368
650    _2
$a zvířata $7 D000818
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a femur $x diagnostické zobrazování $x fyziologie $7 D005269
650    _2
$a lidé $7 D006801
650    _2
$a syndromy imunologické nedostatečnosti $x diagnostické zobrazování $x imunologie $x terapie $7 D007153
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a transplantace mezenchymálních kmenových buněk $x metody $7 D045164
650    _2
$a mezenchymální kmenové buňky $x fyziologie $7 D059630
650    _2
$a lidé středního věku $7 D008875
650    _2
$a osteogeneze $x fyziologie $7 D010012
650    _2
$a náhodné rozdělení $7 D011897
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a potkani nazí $7 D011923
650    _2
$a počítačová rentgenová tomografie $x metody $7 D014057
650    _2
$a výsledek terapie $7 D016896
650    _2
$a hojení ran $x fyziologie $7 D014945
655    _2
$a časopisecké články $7 D016428
700    1_
$a Rentsch, Claudia, $d 1983- $7 xx0209603 $u Translational Bone, Joint and Soft Tissue Research, Medical Faculty and University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
700    1_
$a Soukup, Tomáš, $d 1980- $7 xx0036274 $u Institute of Histology and Embryology, Medical Faculty Hradec Králové, Charles University, Hradec Králové, Czech Republic
700    1_
$a Novotný, Ladislav, $d 1950- $7 xx0105828 $u Radioisotope Laboratory and Animal House, Medical Faculty Hradec Králové, Charles University, Hradec Králové, Czech Republic; Finn Pathologists, One Eyed Lane, Weybread, Norfolk, IP21 5TT, United Kingdom
700    1_
$a Rentsch, B. $u Translational Bone, Joint and Soft Tissue Research, Medical Faculty and University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
700    1_
$a Kanderová, Veronika $7 xx0200317 $u CLIP - Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, Second Medical Faculty, Charles University, Prague and Motol University Hospital, Prague, Prague, Czech Republic
700    1_
$a Rychtrmocová, Hana $7 xx0209604 $u First Department of Medicine, First Medical Faculty, Charles University, Prague and General University Hospital, Prague, Czech Republic
700    1_
$a Kalmárová, Markéta $7 xx0095356 $u First Department of Medicine, First Medical Faculty, Charles University, Prague and General University Hospital, Prague, Czech Republic
700    1_
$a Stehlík, David $7 xx0138007 $u Institute of Anatomy, First Medical Faculty, Charles University, Prague, Czech Republic; Department of Orthopedics, Second Medical Faculty, Charles University, and Motol University Hospital, Prague, Czech Republic
700    1_
$a Trněný, Marek, $d 1960- $7 nlk20000083659 $u First Department of Medicine, First Medical Faculty, Charles University, Prague and General University Hospital, Prague, Czech Republic
700    1_
$a Slanař, Ondřej $7 xx0058262 $u Institute of Pharmacology, First Medical Faculty, Charles University, Prague and General University Hospital, Prague, Czech Republic
773    0_
$w MED00003824 $t Physiological research $x 1802-9973 $g Roč. 66, č. 1 (2017), s. 113-123
856    41
$u http://www.biomed.cas.cz/physiolres/ $y stránka časopisu
910    __
$a ABA008 $b A 4120 $c 266 $y 4 $z 0
990    __
$a 20170824 $b ABA008
991    __
$a 20190514105937 $b ABA008
999    __
$a ok $b bmc $g 1246217 $s 986967
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 66 $c 1 $d 113-123 $e 20161026 $i 1802-9973 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
GRA    __
$a NT13531 $p MZ0
LZP    __
$b NLK118 $a Pubmed-20170824

Najít záznam

Citační ukazatele

Možnosti archivace