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Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma

M. Fedorko, J. Juracek, M. Stanik, M. Svoboda, A. Poprach, T. Buchler, D. Pacik, J. Dolezel, O. Slaby,

. 2017 ; 27 (2) : 411-417.

Language English Country Croatia

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc17030696

Grant support
NV15-31071A MZ0 CEP Register

INTRODUCTION: Urinary microRNAs (miRNAs) are emerging as a clinically useful tool for early and non-invasive detection of various types of cancer. The aim of this study was to evaluate whether let-7 family miRNAs differ in their urinary concentrations between renal cell carcinoma (RCC) cases and healthy controls. MATERIALS AND METHODS: In the case-control study, 69 non-metastatic clear-cell RCC patients and 36 gender/age-matched healthy controls were prospectively enrolled. Total RNA was purified from cell-free supernatant of the 105 first morning urine specimens. Let-7 family miRNAs were determined in cell-free supernatant using quantitative miRNA real-time reverse-transcription PCR and absolute quantification approach. RESULTS: Concentrations of all let-7 miRNAs (let-7a, let-7b, let-7c, let-7d, let-7e and let-7g) were significantly higher in urine samples obtained from RCC patients compared to healthy controls (P < 0.001; P < 0.001; P = 0.005; P = 0.006; P = 0.015 and P = 0.002, respectively). Subsequent ROC analysis has shown that let-7a concentration possesses good ability to differentiate between cases and controls with area under curve being 0.8307 (sensitivity 71%, specificity 81%). CONCLUSIONS: We have shown that let-7 miRNAs are abundant in the urine samples of patients with clear-cell RCC, and out of six let-7 family members, let-7a outperforms the others and presents promising non-invasive biomarker for the detection of RCC.

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$a INTRODUCTION: Urinary microRNAs (miRNAs) are emerging as a clinically useful tool for early and non-invasive detection of various types of cancer. The aim of this study was to evaluate whether let-7 family miRNAs differ in their urinary concentrations between renal cell carcinoma (RCC) cases and healthy controls. MATERIALS AND METHODS: In the case-control study, 69 non-metastatic clear-cell RCC patients and 36 gender/age-matched healthy controls were prospectively enrolled. Total RNA was purified from cell-free supernatant of the 105 first morning urine specimens. Let-7 family miRNAs were determined in cell-free supernatant using quantitative miRNA real-time reverse-transcription PCR and absolute quantification approach. RESULTS: Concentrations of all let-7 miRNAs (let-7a, let-7b, let-7c, let-7d, let-7e and let-7g) were significantly higher in urine samples obtained from RCC patients compared to healthy controls (P < 0.001; P < 0.001; P = 0.005; P = 0.006; P = 0.015 and P = 0.002, respectively). Subsequent ROC analysis has shown that let-7a concentration possesses good ability to differentiate between cases and controls with area under curve being 0.8307 (sensitivity 71%, specificity 81%). CONCLUSIONS: We have shown that let-7 miRNAs are abundant in the urine samples of patients with clear-cell RCC, and out of six let-7 family members, let-7a outperforms the others and presents promising non-invasive biomarker for the detection of RCC.
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$a Juracek, Jaroslav $u Masaryk University, Central European Institute of Technology, Brno, Czech Republic.
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$a Stanik, Michal $u Masaryk Memorial Cancer Institute, Department of Urologic Oncology, Brno, Czech Republic.
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$a Svoboda, Marek $u Masaryk Memorial Cancer Institute, Department of Comprehensive Cancer Care, Brno, Czech Republic.
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$a Poprach, Alexandr $u Masaryk Memorial Cancer Institute, Department of Comprehensive Cancer Care, Brno, Czech Republic.
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$a Buchler, Tomas $u Department of Oncology, Thomayer Hospital and Charles University First Faculty of Medicine, Prague, Czech Republic.
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$a Pacik, Dalibor $u Department of Urology, University Hospital Brno and Masaryk University Brno, Brno, Czech Republic.
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$a Dolezel, Jan $u Masaryk Memorial Cancer Institute, Department of Urologic Oncology, Brno, Czech Republic.
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$a Slaby, Ondrej $u Masaryk University, Central European Institute of Technology, Brno, Czech Republic. Masaryk Memorial Cancer Institute, Department of Comprehensive Cancer Care, Brno, Czech Republic.
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