Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma
Language English Country Croatia Media print
Document type Journal Article
PubMed
28694731
PubMed Central
PMC5493171
DOI
10.11613/bm.2017.043
PII: bm-27-411
Knihovny.cz E-resources
- Keywords
- diagnostic biomarker, let-7, renal cell carcinoma, urine microRNAs,
- MeSH
- Adult MeSH
- Carcinoma, Renal Cell diagnosis genetics urine MeSH
- Middle Aged MeSH
- Humans MeSH
- MicroRNAs genetics urine MeSH
- Biomarkers, Tumor genetics MeSH
- Kidney Neoplasms diagnosis genetics urine MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Gene Expression Regulation, Neoplastic * MeSH
- ROC Curve MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- MicroRNAs MeSH
- mirnlet7 microRNA, human MeSH Browser
- Biomarkers, Tumor MeSH
INTRODUCTION: Urinary microRNAs (miRNAs) are emerging as a clinically useful tool for early and non-invasive detection of various types of cancer. The aim of this study was to evaluate whether let-7 family miRNAs differ in their urinary concentrations between renal cell carcinoma (RCC) cases and healthy controls. MATERIALS AND METHODS: In the case-control study, 69 non-metastatic clear-cell RCC patients and 36 gender/age-matched healthy controls were prospectively enrolled. Total RNA was purified from cell-free supernatant of the 105 first morning urine specimens. Let-7 family miRNAs were determined in cell-free supernatant using quantitative miRNA real-time reverse-transcription PCR and absolute quantification approach. RESULTS: Concentrations of all let-7 miRNAs (let-7a, let-7b, let-7c, let-7d, let-7e and let-7g) were significantly higher in urine samples obtained from RCC patients compared to healthy controls (P < 0.001; P < 0.001; P = 0.005; P = 0.006; P = 0.015 and P = 0.002, respectively). Subsequent ROC analysis has shown that let-7a concentration possesses good ability to differentiate between cases and controls with area under curve being 0.8307 (sensitivity 71%, specificity 81%). CONCLUSIONS: We have shown that let-7 miRNAs are abundant in the urine samples of patients with clear-cell RCC, and out of six let-7 family members, let-7a outperforms the others and presents promising non-invasive biomarker for the detection of RCC.
Department of Urology University Hospital Brno and Masaryk University Brno Brno Czech Republic
Masaryk Memorial Cancer Institute Department of Comprehensive Cancer Care Brno Czech Republic
Masaryk Memorial Cancer Institute Department of Urologic Oncology Brno Czech Republic
Masaryk University Central European Institute of Technology Brno Czech Republic
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