Genome-wide identification of urinary cell-free microRNAs for non-invasive detection of bladder cancer
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
29363887
PubMed Central
PMC5824364
DOI
10.1111/jcmm.13487
Knihovny.cz E-zdroje
- Klíčová slova
- biomarker, bladder cancer, cell-free miRNAs, non-invasive diagnosis, urine,
- MeSH
- celogenomová asociační studie MeSH
- dospělí MeSH
- genom lidský MeSH
- karcinom diagnóza genetika patologie moč MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikro RNA genetika moč MeSH
- nádorové biomarkery genetika moč MeSH
- nádory močového měchýře diagnóza genetika patologie moč MeSH
- prospektivní studie MeSH
- regulace genové exprese u nádorů * MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- studie případů a kontrol MeSH
- stupeň nádoru MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- mikro RNA MeSH
- MIRN191 microRNA, human MeSH Prohlížeč
- MIRN31 microRNA, human MeSH Prohlížeč
- MIRN93 microRNA, human MeSH Prohlížeč
- nádorové biomarkery MeSH
Urinary microRNAs (miRNAs) are emerging as clinically useful tool for early and non-invasive detection of various types of cancer including bladder cancer (BCA). In this study, 205 patients with BCA and 99 healthy controls were prospectively enrolled. Expression profiles of urinary miRNAs were obtained using Affymetrix miRNA microarrays (2578 miRNAs) and candidate miRNAs further validated in independent cohorts using qRT-PCR. Whole-genome profiling identified 76 miRNAs with significantly different concentrations in urine of BCA compared to controls (P < 0.01). In the training and independent validation phase of the study, miR-31-5p, miR-93-5p and miR-191-5p were confirmed to have significantly higher levels in urine of patients with BCA in comparison with controls (P < 0.01). We further established 2-miRNA-based urinary DxScore (miR-93-5p, miR-31-5p) enabling sensitive BCA detection with AUC being 0.84 and 0.81 in the training and validation phase, respectively. Moreover, DxScore significantly differed in the various histopathological subgroups of BCA and decreased post-operatively. In conclusion, we identified and independently validated cell-free urinary miRNAs as promising biomarkers enabling non-invasive detection of BCA.
Central European Institute of Technology Masaryk University Brno Czech Republic
Department of Comprehensive Cancer Care Masaryk Memorial Cancer Institute Brno Czech Republic
Department of Urologic Oncology Masaryk Memorial Cancer Institute Brno Czech Republic
Department of Urology University Hospital Brno Masaryk University Brno Brno Czech Republic
Zobrazit více v PubMed
Antoni S, Ferlay J, Soerjomataram I, PubMed
Goodison S, Rosser C, Urquidi V. Bladder cancer detection and monitoring: assessment of urine‐ and blood‐based marker tests. Mol Diagn Ther. 2013; 17: 71–84. PubMed PMC
Raitanen MP, Aine R, Rintala E, PubMed
Boman H, Hedelin H, Holmäng S. Four bladder tumor markers have a disappointingly low sensitivity for small size and low grade recurrence. J Urol. 2002; 167: 80–3. PubMed
Enokida H, Yoshino H, Matsushita R, PubMed PMC
Weber JA, Baxter DH, Zhang S, PubMed PMC
Mlcochova H, Hezova R, Stanik M, PubMed
R Core Team . R: a language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing; 2014. http://www.R-project.org/.
Fedorko M, Juracek J, Stanik M, PubMed PMC
Hanke M, Hoefig K, Merz H, PubMed
Wang G, Chan E, Kwan B, PubMed
Puerta‐Gil P, García‐Baquero R, Jia AY, PubMed
Pospisilova S, Pazourkova E, Horinek A, PubMed
Urquidi V, Netherton M, Gomes‐Giacoia E, PubMed PMC
Yun S, Jeong P, Kim W, PubMed
De Long J, Sullivan T, Humphrey J, PubMed PMC
Yamada Y, Enokida H, Kojima S, PubMed
Cell-free microRNAs as Non-invasive Diagnostic and Prognostic Bio- markers in Pancreatic Cancer
