Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma
Jazyk angličtina Země Chorvatsko Médium print
Typ dokumentu časopisecké články
PubMed
28694731
PubMed Central
PMC5493171
DOI
10.11613/bm.2017.043
PII: bm-27-411
Knihovny.cz E-zdroje
- Klíčová slova
- diagnostic biomarker, let-7, renal cell carcinoma, urine microRNAs,
- MeSH
- dospělí MeSH
- karcinom z renálních buněk diagnóza genetika moč MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikro RNA genetika moč MeSH
- nádorové biomarkery genetika MeSH
- nádory ledvin diagnóza genetika moč MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- regulace genové exprese u nádorů * MeSH
- ROC křivka MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- mikro RNA MeSH
- mirnlet7 microRNA, human MeSH Prohlížeč
- nádorové biomarkery MeSH
INTRODUCTION: Urinary microRNAs (miRNAs) are emerging as a clinically useful tool for early and non-invasive detection of various types of cancer. The aim of this study was to evaluate whether let-7 family miRNAs differ in their urinary concentrations between renal cell carcinoma (RCC) cases and healthy controls. MATERIALS AND METHODS: In the case-control study, 69 non-metastatic clear-cell RCC patients and 36 gender/age-matched healthy controls were prospectively enrolled. Total RNA was purified from cell-free supernatant of the 105 first morning urine specimens. Let-7 family miRNAs were determined in cell-free supernatant using quantitative miRNA real-time reverse-transcription PCR and absolute quantification approach. RESULTS: Concentrations of all let-7 miRNAs (let-7a, let-7b, let-7c, let-7d, let-7e and let-7g) were significantly higher in urine samples obtained from RCC patients compared to healthy controls (P < 0.001; P < 0.001; P = 0.005; P = 0.006; P = 0.015 and P = 0.002, respectively). Subsequent ROC analysis has shown that let-7a concentration possesses good ability to differentiate between cases and controls with area under curve being 0.8307 (sensitivity 71%, specificity 81%). CONCLUSIONS: We have shown that let-7 miRNAs are abundant in the urine samples of patients with clear-cell RCC, and out of six let-7 family members, let-7a outperforms the others and presents promising non-invasive biomarker for the detection of RCC.
Department of Urology University Hospital Brno and Masaryk University Brno Brno Czech Republic
Masaryk Memorial Cancer Institute Department of Comprehensive Cancer Care Brno Czech Republic
Masaryk Memorial Cancer Institute Department of Urologic Oncology Brno Czech Republic
Masaryk University Central European Institute of Technology Brno Czech Republic
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