Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Hibiscus sabdariffa L. and Its Bioactive Constituents Exhibit Antiviral Activity against HSV-2 and Anti-enzymatic Properties against Urease by an ESI-MS Based Assay

STS. Hassan, E. Švajdlenka, K. Berchová-Bímová,

. 2017 ; 22 (5) : . [pub] 20170430

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc17030839

For decades, Hibiscus sabdariffa L. and its phytochemicals have been shown to possess a wide range of pharmacologic properties. In this study, aqueous extract of Hibiscus sabdariffa (AEHS) and its bioactive constituent protocatechuic acid (PCA), have been evaluated in vitro for their antiviral activity against HSV-2 clinical isolates and anti-enzymatic activity against urease. Antiherpetic activity was evaluated by the titer reduction assay in infected Vero cells, and cytotoxicity was evaluated by the neutral red dye-uptake method. Anti-urease activity was determined by a developed Electrospray Ionization-Mass Spectrometry (ESI-MS)-based assay. PCA showed potent anti-HSV-2 activity compared with that of acyclovir, with EC50 values of 0.92 and 1.43 µg∙mL(-1), respectively, and selectivity indices > 217 and > 140, respectively. For the first time, AEHS was shown to exert anti-urease inhibition activity, with an IC50 value of 82.4 µg∙mL(-1). This, combined with its safety, could facilitate its use in practical applications as a natural urease inhibitor. Our results present Hibiscus sabdariffa L. and its bioactive compound PCA as potential therapeutic agents in the treatment of HSV-2 infection and the treatment of diseases caused by urease-producing bacteria.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17030839
003      
CZ-PrNML
005      
20171030134131.0
007      
ta
008      
171025s2017 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/molecules22050722 $2 doi
035    __
$a (PubMed)28468298
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Hassan, Sherif T S $u Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1946/1, 612 42 Brno, Czech Republic. sherif.hassan@seznam.cz. Department of Applied Ecology, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Kamýcká 129, 165 21 Praha-Suchdol, Czech Republic. sherif.hassan@seznam.cz.
245    10
$a Hibiscus sabdariffa L. and Its Bioactive Constituents Exhibit Antiviral Activity against HSV-2 and Anti-enzymatic Properties against Urease by an ESI-MS Based Assay / $c STS. Hassan, E. Švajdlenka, K. Berchová-Bímová,
520    9_
$a For decades, Hibiscus sabdariffa L. and its phytochemicals have been shown to possess a wide range of pharmacologic properties. In this study, aqueous extract of Hibiscus sabdariffa (AEHS) and its bioactive constituent protocatechuic acid (PCA), have been evaluated in vitro for their antiviral activity against HSV-2 clinical isolates and anti-enzymatic activity against urease. Antiherpetic activity was evaluated by the titer reduction assay in infected Vero cells, and cytotoxicity was evaluated by the neutral red dye-uptake method. Anti-urease activity was determined by a developed Electrospray Ionization-Mass Spectrometry (ESI-MS)-based assay. PCA showed potent anti-HSV-2 activity compared with that of acyclovir, with EC50 values of 0.92 and 1.43 µg∙mL(-1), respectively, and selectivity indices > 217 and > 140, respectively. For the first time, AEHS was shown to exert anti-urease inhibition activity, with an IC50 value of 82.4 µg∙mL(-1). This, combined with its safety, could facilitate its use in practical applications as a natural urease inhibitor. Our results present Hibiscus sabdariffa L. and its bioactive compound PCA as potential therapeutic agents in the treatment of HSV-2 infection and the treatment of diseases caused by urease-producing bacteria.
650    _2
$a acyklovir $x farmakologie $7 D000212
650    _2
$a zvířata $7 D000818
650    _2
$a antivirové látky $x chemie $x izolace a purifikace $x farmakologie $7 D000998
650    _2
$a Cercopithecus aethiops $7 D002522
650    _2
$a vysokoúčinná kapalinová chromatografie $7 D002851
650    _2
$a preklinické hodnocení léčiv $7 D004353
650    _2
$a inhibitory enzymů $x chemie $x izolace a purifikace $x farmakologie $7 D004791
650    _2
$a lidský herpesvirus 2 $x účinky léků $7 D018258
650    _2
$a Hibiscus $x chemie $7 D031584
650    _2
$a inhibiční koncentrace 50 $7 D020128
650    _2
$a kinetika $7 D007700
650    _2
$a rostlinné extrakty $x chemie $x izolace a purifikace $x farmakologie $7 D010936
650    _2
$a polyfenoly $x chemie $x izolace a purifikace $x farmakologie $7 D059808
650    _2
$a hmotnostní spektrometrie s elektrosprejovou ionizací $7 D021241
650    _2
$a ureasa $x antagonisté a inhibitory $x chemie $7 D014510
650    _2
$a Vero buňky $7 D014709
655    _2
$a časopisecké články $7 D016428
700    1_
$a Švajdlenka, Emil $u Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1946/1, 612 42 Brno, Czech Republic. svajdlenkae@vfu.cz.
700    1_
$a Berchová-Bímová, Kateřina $u Department of Applied Ecology, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Kamýcká 129, 165 21 Praha-Suchdol, Czech Republic. berchova@knc.czu.cz.
773    0_
$w MED00180394 $t Molecules (Basel, Switzerland) $x 1420-3049 $g Roč. 22, č. 5 (2017)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/28468298 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20171025 $b ABA008
991    __
$a 20171030134220 $b ABA008
999    __
$a ok $b bmc $g 1254432 $s 991866
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 22 $c 5 $e 20170430 $i 1420-3049 $m Molecules $n Molecules $x MED00180394
LZP    __
$a Pubmed-20171025

Find record

Citation metrics

Archiving options