• Je něco špatně v tomto záznamu ?

Detailed immunophenotyping of B-cell precursors in regenerating bone marrow of acute lymphoblastic leukaemia patients: implications for minimal residual disease detection

PMJ. Theunissen, L. Sedek, V. De Haas, T. Szczepanski, A. Van Der Sluijs, E. Mejstrikova, M. Nováková, T. Kalina, Q. Lecrevisse, A. Orfao, AC. Lankester, JJM. van Dongen, VHJ. Van Der Velden, . ,

. 2017 ; 178 (2) : 257-266. [pub] 20170417

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc17030879

Grantová podpora
NV15-28525A MZ0 CEP - Centrální evidence projektů

Flow cytometric detection of minimal residual disease (MRD) in children with B-cell precursor acute lymphoblastic leukaemia (BCP-ALL) requires immunophenotypic discrimination between residual leukaemic cells and B-cell precursors (BCPs) which regenerate during therapy intervals. In this study, EuroFlow-based 8-colour flow cytometry and innovative analysis tools were used to first characterize the immunophenotypic maturation of normal BCPs in bone marrow (BM) from healthy children, resulting in a continuous multiparametric pathway including transition stages. This pathway was subsequently used as a reference to characterize the immunophenotypic maturation of regenerating BCPs in BM from children treated for BCP-ALL. We identified pre-B-I cells that expressed low or dim CD34 levels, in contrast to the classical CD34(high) pre-B-I cell immunophenotype. These CD34(-dim) pre-B-I cells were relatively abundant in regenerating BM (11-85% within pre-B-I subset), while hardly present in healthy control BM (9-13% within pre-B-I subset; P = 0·0037). Furthermore, we showed that some of the BCP-ALL diagnosis immunophenotypes (23%) overlapped with CD34(-dim) pre-B-I cells. Our results indicate that newly identified CD34(-dim) pre-B-I cells can be mistaken for residual BCP-ALL cells, potentially resulting in false-positive MRD outcomes. Therefore, regenerating BM, in which CD34(-dim) pre-B-I cells are relatively abundant, should be used as reference frame in flow cytometric MRD measurements.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17030879
003      
CZ-PrNML
005      
20201022094417.0
007      
ta
008      
171025s2017 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1111/bjh.14682 $2 doi
035    __
$a (PubMed)28419441
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Theunissen, Prisca M J $u Department of Immunology, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, the Netherlands.
245    10
$a Detailed immunophenotyping of B-cell precursors in regenerating bone marrow of acute lymphoblastic leukaemia patients: implications for minimal residual disease detection / $c PMJ. Theunissen, L. Sedek, V. De Haas, T. Szczepanski, A. Van Der Sluijs, E. Mejstrikova, M. Nováková, T. Kalina, Q. Lecrevisse, A. Orfao, AC. Lankester, JJM. van Dongen, VHJ. Van Der Velden, . ,
520    9_
$a Flow cytometric detection of minimal residual disease (MRD) in children with B-cell precursor acute lymphoblastic leukaemia (BCP-ALL) requires immunophenotypic discrimination between residual leukaemic cells and B-cell precursors (BCPs) which regenerate during therapy intervals. In this study, EuroFlow-based 8-colour flow cytometry and innovative analysis tools were used to first characterize the immunophenotypic maturation of normal BCPs in bone marrow (BM) from healthy children, resulting in a continuous multiparametric pathway including transition stages. This pathway was subsequently used as a reference to characterize the immunophenotypic maturation of regenerating BCPs in BM from children treated for BCP-ALL. We identified pre-B-I cells that expressed low or dim CD34 levels, in contrast to the classical CD34(high) pre-B-I cell immunophenotype. These CD34(-dim) pre-B-I cells were relatively abundant in regenerating BM (11-85% within pre-B-I subset), while hardly present in healthy control BM (9-13% within pre-B-I subset; P = 0·0037). Furthermore, we showed that some of the BCP-ALL diagnosis immunophenotypes (23%) overlapped with CD34(-dim) pre-B-I cells. Our results indicate that newly identified CD34(-dim) pre-B-I cells can be mistaken for residual BCP-ALL cells, potentially resulting in false-positive MRD outcomes. Therefore, regenerating BM, in which CD34(-dim) pre-B-I cells are relatively abundant, should be used as reference frame in flow cytometric MRD measurements.
650    _2
$a mladiství $7 D000293
650    _2
$a antigeny CD34 $x metabolismus $7 D018952
650    _2
$a kostní dřeň $x fyziologie $7 D001853
650    _2
$a buněčná diferenciace $x fyziologie $7 D002454
650    _2
$a předškolní dítě $7 D002675
650    _2
$a průtoková cytometrie $7 D005434
650    _2
$a genová přestavba B-lymfocytů $x imunologie $7 D015322
650    _2
$a lidé $7 D006801
650    _2
$a těžké řetězce imunoglobulinů $x imunologie $7 D007143
650    _2
$a imunofenotypizace $x metody $7 D016130
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a reziduální nádor $7 D018365
650    _2
$a pre-B-buněčná leukemie $x diagnóza $x imunologie $x patofyziologie $7 D015452
650    _2
$a prekurzorové B-lymfoidní buňky $x imunologie $x patologie $x fyziologie $7 D054448
650    _2
$a regenerace $7 D012038
655    _2
$a časopisecké články $7 D016428
700    1_
$a Sedek, Lukasz $u Department of Paediatric Haematology and Oncology, Zabrze, Poland. Medical University of Silesia (SUM), Katowice, Poland.
700    1_
$a De Haas, Valerie $u Dutch Childhood Oncology Group, The Hague, the Netherlands.
700    1_
$a Szczepanski, Tomasz $u Department of Paediatric Haematology and Oncology, Zabrze, Poland. Medical University of Silesia (SUM), Katowice, Poland.
700    1_
$a Van Der Sluijs, Alita $u Dutch Childhood Oncology Group, The Hague, the Netherlands.
700    1_
$a Mejstrikova, Ester $u Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University (DPH/O) and University Hospital Motol, Prague, Czech Republic.
700    1_
$a Nováková, Michaela $u Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University (DPH/O) and University Hospital Motol, Prague, Czech Republic.
700    1_
$a Kalina, Tomas $u Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University (DPH/O) and University Hospital Motol, Prague, Czech Republic.
700    1_
$a Lecrevisse, Quentin $u Cancer Research Centre (IBMCC-CSIC), Department of Medicine and Cytometry Service, University of Salamanca (USAL) and Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain.
700    1_
$a Orfao, Alberto $u Cancer Research Centre (IBMCC-CSIC), Department of Medicine and Cytometry Service, University of Salamanca (USAL) and Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain.
700    1_
$a Lankester, Arjan C $u Department of Paediatrics, Leiden University Medical Centre, Leiden, the Netherlands.
700    1_
$a van Dongen, Jacques J M $u Department of Immunology, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, the Netherlands. Department of Immunohaematology and Blood Transfusion, Leiden University Medical Centre, Leiden, the Netherlands.
700    1_
$a Van Der Velden, Vincent H J $u Department of Immunology, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, the Netherlands.
700    1_
$a ,
773    0_
$w MED00009374 $t British journal of haematology $x 1365-2141 $g Roč. 178, č. 2 (2017), s. 257-266
856    41
$u https://pubmed.ncbi.nlm.nih.gov/28419441 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20171025 $b ABA008
991    __
$a 20201022094415 $b ABA008
999    __
$a ok $b bmc $g 1254472 $s 991906
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 178 $c 2 $d 257-266 $e 20170417 $i 1365-2141 $m British journal of haematology $n Br J Haematol $x MED00009374
GRA    __
$a NV15-28525A $p MZ0
LZP    __
$a Pubmed-20171025

Najít záznam

Citační ukazatele

Možnosti archivace