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Fast and sensitive analysis of beta blockers by ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry

J. Tomková, P. Ondra, E. Kocianová, J. Václavík,

. 2017 ; 31 (7) : . [pub] 20170104

Language English Country England, Great Britain

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc17031240

This paper presents a method for the determination of acebutolol, betaxolol, bisoprolol, metoprolol, nebivolol and sotalol in human serum by liquid-liquid extraction and ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry. After liquid-liquid extraction, beta blockers were separated on a reverse-phase analytical column (Acclaim RS 120; 100 × 2.1 mm, 2.2 μm). The total run time was 6 min for each sample. Linearity, limit of detection, limit of quantification, matrix effects, specificity, precision, accuracy, recovery and sample stability were evaluated. The method was successfully applied to the therapeutic drug monitoring of 108 patients with hypertension. This method was also used for determination of beta blockers in 33 intoxicated patients.

References provided by Crossref.org

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$a This paper presents a method for the determination of acebutolol, betaxolol, bisoprolol, metoprolol, nebivolol and sotalol in human serum by liquid-liquid extraction and ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry. After liquid-liquid extraction, beta blockers were separated on a reverse-phase analytical column (Acclaim RS 120; 100 × 2.1 mm, 2.2 μm). The total run time was 6 min for each sample. Linearity, limit of detection, limit of quantification, matrix effects, specificity, precision, accuracy, recovery and sample stability were evaluated. The method was successfully applied to the therapeutic drug monitoring of 108 patients with hypertension. This method was also used for determination of beta blockers in 33 intoxicated patients.
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$a Ondra, Peter $u Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic. Department of Forensic Medicine and Medical Law, University Hospital Olomouc, Olomouc, Czech Republic.
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$a Kocianová, Eva $u Department of Forensic Medicine and Medical Law, University Hospital Olomouc, Olomouc, Czech Republic. Department of Internal Medicine I - Cardiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic.
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$a Václavík, Jan $u Department of Forensic Medicine and Medical Law, University Hospital Olomouc, Olomouc, Czech Republic. Department of Internal Medicine I - Cardiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic.
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