-
Something wrong with this record ?
Molecular Characterization of OXA-48-Like-Producing Enterobacteriaceae in the Czech Republic and Evidence for Horizontal Transfer of pOXA-48-Like Plasmids
A. Skalova, K. Chudejova, V. Rotova, M. Medvecky, V. Studentova, E. Chudackova, P. Lavicka, T. Bergerova, V. Jakubu, H. Zemlickova, CC. Papagiannitsis, J. Hrabak,
Language English Country United States
Document type Journal Article
Grant support
NV15-28663A
MZ0
CEP Register
Digital library NLK
Full text - Article
Source
NLK
Free Medical Journals
from 1972 to 6 months ago
Freely Accessible Science Journals
from 1995 to 6 months ago
PubMed Central
from 1972 to 1 year ago
Europe PubMed Central
from 1972 to 6 months ago
Open Access Digital Library
from 1972-01-01
Open Access Digital Library
from 1972-01-01
PubMed
27855076
DOI
10.1128/aac.01889-16
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Chromosomes, Bacterial genetics MeSH
- Bacterial Proteins genetics metabolism MeSH
- beta-Lactamases genetics metabolism MeSH
- Enterobacteriaceae drug effects enzymology genetics MeSH
- Escherichia coli drug effects enzymology genetics MeSH
- Klebsiella pneumoniae drug effects enzymology genetics MeSH
- Microbial Sensitivity Tests MeSH
- Plasmids genetics MeSH
- Gene Transfer, Horizontal genetics MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
The aim of this study was to characterize the first cases and outbreaks of OXA-48-like-producing Enterobacteriaceae recovered from hospital settings in the Czech Republic. From 2013 to 2015, 22 Klebsiella pneumoniae isolates, 3 Escherichia coli isolates, and 1 Enterobacter cloacae isolate producing OXA-48-like carbapenemases were isolated from 20 patients. Four of the patients were colonized or infected by two or three different OXA-48-like producers. The K. pneumoniae isolates were classified into nine sequence types (STs), with ST101 being predominant (n = 8). The E. coli isolates were of different STs, while the E. cloacae isolate belonged to ST109. Twenty-four isolates carried blaOXA-48, while two isolates carried blaOXA-181 or blaOXA-232 Almost all isolates (n = 22) carried blaOXA-48-positive plasmids of a similar size (∼60 kb), except the two isolates producing OXA-181 or OXA-232. In an ST45 K. pneumoniae isolate and an ST38 E. coli isolate, S1 nuclease profiling plus hybridization indicated a chromosomal location of blaOXA-48 Sequencing showed that the majority of blaOXA-48-carrying plasmids exhibited high degrees of identity with the pOXA-48-like plasmid pE71T. Additionally, two novel pE71T derivatives, pOXA-48_30715 and pOXA-48_30891, were observed. The blaOXA-181-carrying plasmid was identical to the IncX3 plasmid pOXA181_EC14828, while the blaOXA-232-carrying plasmid was a ColE2-type plasmid, being a novel derivative of pOXA-232. Finally, sequencing data showed that the ST45 K. pneumoniae and ST38 E. coli isolates harbored the IS1R-based composite transposon Tn6237 containing blaOXA-48 integrated into their chromosomes. These findings underlined that the horizontal transfer of pOXA-48-like plasmids has played a major role in the dissemination of blaOXA-48 in the Czech Republic. In combination with the difficulties with their detection, OXA-48 producers constitute an important public threat.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc17031287
- 003
- CZ-PrNML
- 005
- 20201022162235.0
- 007
- ta
- 008
- 171025s2017 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1128/AAC.01889-16 $2 doi
- 035 __
- $a (PubMed)27855076
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Skalova, Anna $u Department of Microbiology, Faculty of Medicine, and University Hospital Pilsen, Charles University, Pilsen, Czech Republic. Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic.
- 245 10
- $a Molecular Characterization of OXA-48-Like-Producing Enterobacteriaceae in the Czech Republic and Evidence for Horizontal Transfer of pOXA-48-Like Plasmids / $c A. Skalova, K. Chudejova, V. Rotova, M. Medvecky, V. Studentova, E. Chudackova, P. Lavicka, T. Bergerova, V. Jakubu, H. Zemlickova, CC. Papagiannitsis, J. Hrabak,
- 520 9_
- $a The aim of this study was to characterize the first cases and outbreaks of OXA-48-like-producing Enterobacteriaceae recovered from hospital settings in the Czech Republic. From 2013 to 2015, 22 Klebsiella pneumoniae isolates, 3 Escherichia coli isolates, and 1 Enterobacter cloacae isolate producing OXA-48-like carbapenemases were isolated from 20 patients. Four of the patients were colonized or infected by two or three different OXA-48-like producers. The K. pneumoniae isolates were classified into nine sequence types (STs), with ST101 being predominant (n = 8). The E. coli isolates were of different STs, while the E. cloacae isolate belonged to ST109. Twenty-four isolates carried blaOXA-48, while two isolates carried blaOXA-181 or blaOXA-232 Almost all isolates (n = 22) carried blaOXA-48-positive plasmids of a similar size (∼60 kb), except the two isolates producing OXA-181 or OXA-232. In an ST45 K. pneumoniae isolate and an ST38 E. coli isolate, S1 nuclease profiling plus hybridization indicated a chromosomal location of blaOXA-48 Sequencing showed that the majority of blaOXA-48-carrying plasmids exhibited high degrees of identity with the pOXA-48-like plasmid pE71T. Additionally, two novel pE71T derivatives, pOXA-48_30715 and pOXA-48_30891, were observed. The blaOXA-181-carrying plasmid was identical to the IncX3 plasmid pOXA181_EC14828, while the blaOXA-232-carrying plasmid was a ColE2-type plasmid, being a novel derivative of pOXA-232. Finally, sequencing data showed that the ST45 K. pneumoniae and ST38 E. coli isolates harbored the IS1R-based composite transposon Tn6237 containing blaOXA-48 integrated into their chromosomes. These findings underlined that the horizontal transfer of pOXA-48-like plasmids has played a major role in the dissemination of blaOXA-48 in the Czech Republic. In combination with the difficulties with their detection, OXA-48 producers constitute an important public threat.
- 650 _2
- $a antibakteriální látky $x farmakologie $7 D000900
- 650 _2
- $a bakteriální proteiny $x genetika $x metabolismus $7 D001426
- 650 _2
- $a bakteriální chromozomy $x genetika $7 D002876
- 650 _2
- $a Česká republika $7 D018153
- 650 _2
- $a Enterobacteriaceae $x účinky léků $x enzymologie $x genetika $7 D004755
- 650 _2
- $a Escherichia coli $x účinky léků $x enzymologie $x genetika $7 D004926
- 650 _2
- $a přenos genů horizontální $x genetika $7 D022761
- 650 _2
- $a Klebsiella pneumoniae $x účinky léků $x enzymologie $x genetika $7 D007711
- 650 _2
- $a mikrobiální testy citlivosti $7 D008826
- 650 _2
- $a plazmidy $x genetika $7 D010957
- 650 _2
- $a beta-laktamasy $x genetika $x metabolismus $7 D001618
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Chudejova, Katerina $u Department of Microbiology, Faculty of Medicine, and University Hospital Pilsen, Charles University, Pilsen, Czech Republic. Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic.
- 700 1_
- $a Rotova, Veronika $u Department of Microbiology, Faculty of Medicine, and University Hospital Pilsen, Charles University, Pilsen, Czech Republic. Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic.
- 700 1_
- $a Medvecky, Matej $u Veterinary Research Institute, Brno, Czech Republic.
- 700 1_
- $a Studentova, Vendula $u Department of Microbiology, Faculty of Medicine, and University Hospital Pilsen, Charles University, Pilsen, Czech Republic. Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic.
- 700 1_
- $a Chudackova, Eva $u Department of Microbiology, Faculty of Medicine, and University Hospital Pilsen, Charles University, Pilsen, Czech Republic. Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic.
- 700 1_
- $a Lavicka, Pavel $u Department of Neurosurgery, Faculty of Medicine, and University Hospital Pilsen, Charles University, Pilsen, Czech Republic.
- 700 1_
- $a Bergerova, Tamara $u Department of Microbiology, Faculty of Medicine, and University Hospital Pilsen, Charles University, Pilsen, Czech Republic. Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic.
- 700 1_
- $a Jakubu, Vladislav $u Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic. National Reference Laboratory for Antibiotics, National Institute of Public Health, Prague, Czech Republic.
- 700 1_
- $a Zemlickova, Helena $u Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic. National Reference Laboratory for Antibiotics, National Institute of Public Health, Prague, Czech Republic. Department of Clinical Microbiology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic.
- 700 1_
- $a Papagiannitsis, Costas C $u Department of Microbiology, Faculty of Medicine, and University Hospital Pilsen, Charles University, Pilsen, Czech Republic c.papagiannitsis@gmail.com. Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic.
- 700 1_
- $a Hrabak, Jaroslav $u Department of Microbiology, Faculty of Medicine, and University Hospital Pilsen, Charles University, Pilsen, Czech Republic. Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic.
- 773 0_
- $w MED00009215 $t Antimicrobial agents and chemotherapy $x 1098-6596 $g Roč. 61, č. 2 (2017)
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/27855076 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20171025 $b ABA008
- 991 __
- $a 20201022162233 $b ABA008
- 999 __
- $a ok $b bmc $g 1254880 $s 992314
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 61 $c 2 $e 20170124 $i 1098-6596 $m Antimicrobial agents and chemotherapy $n Antimicrob Agents Chemother $x MED00009215
- GRA __
- $a NV15-28663A $p MZ0
- LZP __
- $a Pubmed-20171025
