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Capillary electrophoresis in the analysis of biologically important thiols
J. Lačná, F. Foret, P. Kubáň,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články, přehledy
PubMed
27611491
DOI
10.1002/elps.201600354
Knihovny.cz E-zdroje
- MeSH
- elektrochemické techniky MeSH
- elektroforéza kapilární metody MeSH
- fluorescence MeSH
- fluorescenční barviva MeSH
- fluorescenční spektrometrie metody MeSH
- hmotnostní spektrometrie MeSH
- konformace proteinů MeSH
- kovové nanočástice MeSH
- lidé MeSH
- metabolomika MeSH
- oxidace-redukce MeSH
- proteiny chemie MeSH
- senzitivita a specificita MeSH
- sulfhydrylové sloučeniny analýza chemie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
In this review article, CE methods for analysis of biologically important thiols are overviewed. The article covers the period from the previously published comprehensive review in 2004 until mid-2016, with emphasis on various detection modes, novel approaches for sample preconcentration, and applications in clinical practice. The most commonly used detection methods, such as conductometry or absorbance detection, although universally applicable and available in most commercial instruments have low sensitivity and have only limited use in thiol analysis. Amperometric and MS detection are more sensitive and have their steady place in thiol analysis, although the mainstay remains CE with LIF detection, reaching nanomolar concentration sensitivities for most of the thiols. Novel probes for CE-LIF have been developed and tested. The preconcentration approaches using modified gold nanoparticles reaching excellent sensitivities in the picomolar range and various sample stacking methods are also reviewed. Finally, significant clinical applications of the developed methods are discussed with critical insights into the future of CE analysis of thiols.
Citace poskytuje Crossref.org
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