• Je něco špatně v tomto záznamu ?

The Supportive Role of Insulin-Like Growth Factor-I in the Differentiation of Murine Mesenchymal Stem Cells into Corneal-Like Cells

P. Trosan, E. Javorkova, A. Zajicova, M. Hajkova, B. Hermankova, J. Kossl, M. Krulova, V. Holan,

. 2016 ; 25 (11) : 874-81. [pub] 20160509

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc17031805

This study was focused on characterizing the differentiation of bone marrow-derived mesenchymal stem cells (MSCs) into corneal-like cells. Mouse MSCs were isolated from the bone marrow, grown in cell culture for 3 weeks, and purified using a magnetic activated cell sorter. Purified MSCs were cultured with an extract prepared from excised corneas and in the presence or absence of insulin-like growth factor-I (IGF-I). Analysis by quantitative real-time polymerase chain reaction showed that the expression of corneal specific markers, such as cytokeratin 12 (K12), keratocan, and lumican, was already induced after a 3-day cultivation and gradually increased during the 10-day incubation of MSCs with the extract. The presence of IGF-I significantly increased differentiation. Immunofluorescence analysis of differentiated MSCs showed positive results for the K12 protein. The morphology of the differentiated cells and the expression of cell surface markers CD45, CD11b, CD73, CD44, and CD105 were comparable in the control and differentiated MSCs. Proliferative activity was even higher in differentiated cells than in untreated MSCs. Both untreated and differentiated MSCs inhibited the production of interleukin-2 and interferon-γ in spleen cells stimulated with Concanavalin A. The results thus show that MSCs cultured in the presence of corneal extract and IGF-I efficiently differentiate into corneal-like cells. The differentiated cells possess characteristics of corneal epithelial cells and keratocytes, while at the same time maintaining MSC properties.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17031805
003      
CZ-PrNML
005      
20171102132526.0
007      
ta
008      
171025s2016 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1089/scd.2016.0030 $2 doi
035    __
$a (PubMed)27050039
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Trosan, Peter $u 1 Department of Transplantation Immunology, Institute of Experimental Medicine , Academy of Sciences of the Czech Republic, Prague, Czech Republic . 2 Department of Cell Biology, Faculty of Science, Charles University , Prague, Czech Republic . 3 Laboratory of the Biology and Pathology of the Eye, First Faculty of Medicine, Institute of Inherited Metabolic Disorders, General University Hospital in Prague, Charles University in Prague , Prague, Czech Republic .
245    14
$a The Supportive Role of Insulin-Like Growth Factor-I in the Differentiation of Murine Mesenchymal Stem Cells into Corneal-Like Cells / $c P. Trosan, E. Javorkova, A. Zajicova, M. Hajkova, B. Hermankova, J. Kossl, M. Krulova, V. Holan,
520    9_
$a This study was focused on characterizing the differentiation of bone marrow-derived mesenchymal stem cells (MSCs) into corneal-like cells. Mouse MSCs were isolated from the bone marrow, grown in cell culture for 3 weeks, and purified using a magnetic activated cell sorter. Purified MSCs were cultured with an extract prepared from excised corneas and in the presence or absence of insulin-like growth factor-I (IGF-I). Analysis by quantitative real-time polymerase chain reaction showed that the expression of corneal specific markers, such as cytokeratin 12 (K12), keratocan, and lumican, was already induced after a 3-day cultivation and gradually increased during the 10-day incubation of MSCs with the extract. The presence of IGF-I significantly increased differentiation. Immunofluorescence analysis of differentiated MSCs showed positive results for the K12 protein. The morphology of the differentiated cells and the expression of cell surface markers CD45, CD11b, CD73, CD44, and CD105 were comparable in the control and differentiated MSCs. Proliferative activity was even higher in differentiated cells than in untreated MSCs. Both untreated and differentiated MSCs inhibited the production of interleukin-2 and interferon-γ in spleen cells stimulated with Concanavalin A. The results thus show that MSCs cultured in the presence of corneal extract and IGF-I efficiently differentiate into corneal-like cells. The differentiated cells possess characteristics of corneal epithelial cells and keratocytes, while at the same time maintaining MSC properties.
650    _2
$a zvířata $7 D000818
650    _2
$a biologické markery $x metabolismus $7 D015415
650    _2
$a buněčná diferenciace $x účinky léků $x genetika $7 D002454
650    _2
$a proliferace buněk $x účinky léků $x genetika $7 D049109
650    _2
$a tvar buňky $x účinky léků $x genetika $7 D048430
650    _2
$a rohovka $x cytologie $7 D003315
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a regulace genové exprese $x účinky léků $7 D005786
650    _2
$a imunosupresivní léčba $7 D007165
650    _2
$a insulinu podobný růstový faktor I $x farmakologie $7 D007334
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a mezenchymální kmenové buňky $x cytologie $x účinky léků $x metabolismus $7 D059630
650    _2
$a myši inbrední BALB C $7 D008807
655    _2
$a časopisecké články $7 D016428
700    1_
$a Javorkova, Eliska $u 1 Department of Transplantation Immunology, Institute of Experimental Medicine , Academy of Sciences of the Czech Republic, Prague, Czech Republic . 2 Department of Cell Biology, Faculty of Science, Charles University , Prague, Czech Republic .
700    1_
$a Zajicova, Alena $u 1 Department of Transplantation Immunology, Institute of Experimental Medicine , Academy of Sciences of the Czech Republic, Prague, Czech Republic .
700    1_
$a Hajkova, Michaela $u 1 Department of Transplantation Immunology, Institute of Experimental Medicine , Academy of Sciences of the Czech Republic, Prague, Czech Republic . 2 Department of Cell Biology, Faculty of Science, Charles University , Prague, Czech Republic .
700    1_
$a Hermankova, Barbora $u 1 Department of Transplantation Immunology, Institute of Experimental Medicine , Academy of Sciences of the Czech Republic, Prague, Czech Republic . 2 Department of Cell Biology, Faculty of Science, Charles University , Prague, Czech Republic .
700    1_
$a Kossl, Jan $u 1 Department of Transplantation Immunology, Institute of Experimental Medicine , Academy of Sciences of the Czech Republic, Prague, Czech Republic . 2 Department of Cell Biology, Faculty of Science, Charles University , Prague, Czech Republic .
700    1_
$a Krulova, Magdalena $u 1 Department of Transplantation Immunology, Institute of Experimental Medicine , Academy of Sciences of the Czech Republic, Prague, Czech Republic . 2 Department of Cell Biology, Faculty of Science, Charles University , Prague, Czech Republic .
700    1_
$a Holan, Vladimir $u 1 Department of Transplantation Immunology, Institute of Experimental Medicine , Academy of Sciences of the Czech Republic, Prague, Czech Republic . 2 Department of Cell Biology, Faculty of Science, Charles University , Prague, Czech Republic .
773    0_
$w MED00162608 $t Stem cells and development $x 1557-8534 $g Roč. 25, č. 11 (2016), s. 874-81
856    41
$u https://pubmed.ncbi.nlm.nih.gov/27050039 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20171025 $b ABA008
991    __
$a 20171102132619 $b ABA008
999    __
$a ok $b bmc $g 1255398 $s 992832
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2016 $b 25 $c 11 $d 874-81 $e 20160509 $i 1557-8534 $m Stem cells and development $n Stem Cells Dev $x MED00162608
LZP    __
$a Pubmed-20171025

Najít záznam

Citační ukazatele

Možnosti archivace