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Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy
J. Jaros, M. Petrov, M. Tesarova, A. Hampl,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
- MeSH
- buněčné sféroidy ultrastruktura MeSH
- embryonální kmenové buňky ultrastruktura MeSH
- kultivované buňky MeSH
- lidé MeSH
- mikroskopie elektronová rastrovací metody MeSH
- počítačové zpracování obrazu MeSH
- zobrazování trojrozměrné metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Cell culture methods have been developed in efforts to produce biologically relevant systems for developmental and disease modeling, and appropriate analytical tools are essential. Knowledge of ultrastructural characteristics represents the basis to reveal in situ the cellular morphology, cell-cell interactions, organelle distribution, niches in which cells reside, and many more. The traditional method for 3D visualization of ultrastructural components, serial sectioning using transmission electron microscopy (TEM), is very labor-intensive due to contentious TEM slice preparation and subsequent image processing of the whole collection. In this chapter, we present serial block-face scanning electron microscopy, together with complex methodology for spheroid formation, contrasting of cellular compartments, image processing, and 3D visualization. The described technique is effective for detailed morphological analysis of stem cell spheroids, organoids, as well as organotypic cell cultures.
CEITEC BUT Brno University of Technology Purkynova 123 612 00 Brno Czech Republic
TESCAN Brno s r o Libusina Trida 1 623 00 Brno Czech Republic
Citace poskytuje Crossref.org
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- $a Jaros, Josef $u Faculty of Medicine, Department of Histology and Embryology, Masaryk University, Kamenice 3/A1, 625 00, Brno, Czech Republic. jaros@med.muni.cz. Cell and Tissue Regeneration, International Clinical Research Center, St. Anne's University Hospital Brno, Pekarska 53, 656 91, Brno, Czech Republic. jaros@med.muni.cz.
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- $a Cell culture methods have been developed in efforts to produce biologically relevant systems for developmental and disease modeling, and appropriate analytical tools are essential. Knowledge of ultrastructural characteristics represents the basis to reveal in situ the cellular morphology, cell-cell interactions, organelle distribution, niches in which cells reside, and many more. The traditional method for 3D visualization of ultrastructural components, serial sectioning using transmission electron microscopy (TEM), is very labor-intensive due to contentious TEM slice preparation and subsequent image processing of the whole collection. In this chapter, we present serial block-face scanning electron microscopy, together with complex methodology for spheroid formation, contrasting of cellular compartments, image processing, and 3D visualization. The described technique is effective for detailed morphological analysis of stem cell spheroids, organoids, as well as organotypic cell cultures.
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