The nitrification of the liquid phase of digestate (LPD) was conducted using a 5L completely stirred tank reactor (CSTR) in two independent periods (P1 - without pH control; P2 - with pH control). The possibility of minimizing nitrogen losses during the application of LPD to the soil as well as during long-term storage or thermal thickening of LPD using nitrification was discussed. Moreover, the feasibility of applying the nitrification of LPD to the production of electron acceptors for biological desulfurization of biogas was assessed. Despite an extremely high average concentration of ammonia and COD in LPD reaching 2470 and 9080mg/L, respectively, nitrification was confirmed immediately after the start-up of the CSTR. N-NO3-concentration reached 250mg/L only two days after the start of P1. On the other hand, P1 demonstrated that working without pH control is a risk because of the free nitrous acid (FNA) inhibition towards nitrite oxidizing bacteria (NOB) resulting in massive nitrite accumulation. Up to 30.9mg/L of FNA was present in the reactor during P1, where the NOB started to be inhibited even at 0.15mg/L of FNA. During P2, the control of pH at 7.0 resulted in nitrogen oxidation efficiency reaching 98.3±1.5% and the presence of N-NO3-among oxidized nitrogen 99.6±0.4%. The representation of volatile free ammonia within total nitrogen was reduced more than 1000 times comparing with raw LPD under these conditions. Thus, optimum characteristics of the tested system from the point of view of minimizing the nitrogen losses as well as production of electron acceptors for the desulfurization of biogas were gained in this phase of reactor operation. Based on the results of the experiments, potential improvements and modifications of the tested system were suggested.

Department of Agro Environmental Chemistry and Plant Nutrition Czech University of Life Sciences Prague Kamycka 129 Prague 165 21 Czech Republic

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