• Something wrong with this record ?

Inducible protein stabilization system in Leishmania mexicana

L. Podešvová, H. Huang, V. Yurchenko,

. 2017 ; 214 (-) : 62-64. [pub] 20170331

Language English Country Netherlands

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc18010704

Targeted regulation of protein levels is an important tool to investigate the role of proteins essential for cell function and development. In recent years, methods based on the Escherichia coli dihydrofolate reductase destabilization domain (ecDHFR DD) have been established and used in various cell types. ecDHFR DD destabilizes the fused protein of interest and causes its degradation by proteasomes, unless it is stabilized by a specific ligand, trimethoprim. In this work we developed an inducible protein stabilization system in Leishmania mexicana based on ecDHFR DD.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18010704
003      
CZ-PrNML
005      
20180420093913.0
007      
ta
008      
180404s2017 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.molbiopara.2017.03.008 $2 doi
035    __
$a (PubMed)28373094
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Podešvová, Lucie $u Life Science Research Centre, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic.
245    10
$a Inducible protein stabilization system in Leishmania mexicana / $c L. Podešvová, H. Huang, V. Yurchenko,
520    9_
$a Targeted regulation of protein levels is an important tool to investigate the role of proteins essential for cell function and development. In recent years, methods based on the Escherichia coli dihydrofolate reductase destabilization domain (ecDHFR DD) have been established and used in various cell types. ecDHFR DD destabilizes the fused protein of interest and causes its degradation by proteasomes, unless it is stabilized by a specific ligand, trimethoprim. In this work we developed an inducible protein stabilization system in Leishmania mexicana based on ecDHFR DD.
650    _2
$a Escherichia coli $x enzymologie $x genetika $7 D004926
650    12
$a regulace genové exprese $7 D005786
650    _2
$a Leishmania mexicana $x genetika $x metabolismus $7 D007894
650    _2
$a molekulární biologie $x metody $7 D008967
650    _2
$a parazitologie $x metody $7 D010274
650    _2
$a rekombinantní proteiny $x genetika $x metabolismus $7 D011994
650    _2
$a dihydrofolátreduktasa $x genetika $x metabolismus $7 D013762
650    12
$a aktivace transkripce $7 D015533
650    _2
$a trimethoprim $x metabolismus $7 D014295
655    _2
$a časopisecké články $7 D016428
700    1_
$a Huang, Huan $u Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
700    1_
$a Yurchenko, Vyacheslav $u Life Science Research Centre, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic; Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 370 05 České Budějovice (Budweis), Czech Republic; Institute of Environmental Technologies, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic. Electronic address: vyacheslav.yurchenko@osu.cz.
773    0_
$w MED00003384 $t Molecular and biochemical parasitology $x 1872-9428 $g Roč. 214, č. - (2017), s. 62-64
856    41
$u https://pubmed.ncbi.nlm.nih.gov/28373094 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20180404 $b ABA008
991    __
$a 20180420094016 $b ABA008
999    __
$a ok $b bmc $g 1288189 $s 1007516
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 214 $c - $d 62-64 $e 20170331 $i 1872-9428 $m Molecular and biochemical parasitology $n Mol Biochem Parasitol $x MED00003384
LZP    __
$a Pubmed-20180404
Back

Find record

Citation metrics

Archiving options