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Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface
M. Naseem, S. Shams, T. Roitsch,
Language English Country United States
Document type Journal Article
- MeSH
- Cytokinins metabolism MeSH
- Phenotype MeSH
- Plants, Genetically Modified MeSH
- Plant Immunity MeSH
- Host-Pathogen Interactions * immunology MeSH
- Plant Diseases genetics immunology microbiology MeSH
- Gene Expression Regulation, Plant MeSH
- Plant Growth Regulators metabolism MeSH
- Signal Transduction MeSH
- Transformation, Genetic MeSH
- Publication type
- Journal Article MeSH
Cytokinins are adenine and non-adenine derived heterogeneous class of regulatory molecules that participate in almost every aspect of plant biology. They also affect plant defense responses as well as help microbial pathogens to establish pathogenesis. The functional approaches that ensure desired and subtle modulations in the levels of plant cytokinins are highly instrumental in assessing their functions in plant immunity. Here, we describe a detailed working protocol regarding the enhanced production of cytokinins from plants that harbor isopentenyltransferase (IPT) enzyme gene under the control of 4xJERE (jasmonic acid and elicitor-responsive element) pathogen-inducible promoter. Our devised expression system is a context-dependent solution when it comes to investigating host-pathogen interactions under the modulated conditions of plant cytokinins.
References provided by Crossref.org
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- $a Naseem, Muhammad $u Deptartment of Bioinformatics, Biocenter University of Würzburg Am Hubland Würzburg Germany, Julius-Maximilians-Universität Würzburg, Würzburg, Germany. muhammad@biozentrum.uni-wuerzburg.de. Department of Molecular Biology and Genetics, Boğaziçi University, North Campus Kuzey Park Building 310, 34342, Bebek, Instanbul, Turkey. muhammad@biozentrum.uni-wuerzburg.de.
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- $a Cytokinins are adenine and non-adenine derived heterogeneous class of regulatory molecules that participate in almost every aspect of plant biology. They also affect plant defense responses as well as help microbial pathogens to establish pathogenesis. The functional approaches that ensure desired and subtle modulations in the levels of plant cytokinins are highly instrumental in assessing their functions in plant immunity. Here, we describe a detailed working protocol regarding the enhanced production of cytokinins from plants that harbor isopentenyltransferase (IPT) enzyme gene under the control of 4xJERE (jasmonic acid and elicitor-responsive element) pathogen-inducible promoter. Our devised expression system is a context-dependent solution when it comes to investigating host-pathogen interactions under the modulated conditions of plant cytokinins.
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- $a Shams, Shabana $u Department of Animal Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
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- $a Roitsch, Thomas $u Department of Plant and Environmental Sciences, Copenhagen Plant Science Centre, University of Copenhagen, Taastrup, Denmark. Global Change Research Centre, Czech Globe AS CR, v.v.i., Drásov 470, 664 24, Drásov, Czech Republic.
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