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A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland
Z. Koledova, P. Lu,
Language English Country United States
Document type Journal Article
- MeSH
- Cellular Microenvironment physiology MeSH
- Stromal Cells cytology MeSH
- Epithelium physiology MeSH
- Epithelial Cells cytology MeSH
- Extracellular Matrix physiology MeSH
- Fibroblasts cytology MeSH
- Coculture Techniques methods MeSH
- Cells, Cultured MeSH
- Mammary Glands, Animal cytology MeSH
- Morphogenesis physiology MeSH
- Mice MeSH
- Signal Transduction physiology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
The mammary gland consists of numerous tissue compartments, including mammary epithelium, an array of stromal cells, and the extracellular matrix (ECM). Bidirectional interactions between the epithelium and its surrounding stroma are essential for proper mammary gland development and homeostasis, whereas their deregulation leads to developmental abnormalities and cancer. To study the relationships between the epithelium and the stroma, development of models that could recapitulate essential aspects of these interacting systems in vitro has become necessary. Here we describe a three-dimensional (3D) co-culture assay and show that the addition of fibroblasts to mammary organoid cultures promotes the epithelium to undergo branching morphogenesis, thus allowing the role of the stromal microenvironment to be examined in this essential developmental process.
References provided by Crossref.org
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- $a Koledova, Zuzana $u Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, UK. zkoledova@gmail.com. Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 3, Brno, 625 00, Czech Republic. zkoledova@gmail.com.
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- $a A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland / $c Z. Koledova, P. Lu,
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- $a The mammary gland consists of numerous tissue compartments, including mammary epithelium, an array of stromal cells, and the extracellular matrix (ECM). Bidirectional interactions between the epithelium and its surrounding stroma are essential for proper mammary gland development and homeostasis, whereas their deregulation leads to developmental abnormalities and cancer. To study the relationships between the epithelium and the stroma, development of models that could recapitulate essential aspects of these interacting systems in vitro has become necessary. Here we describe a three-dimensional (3D) co-culture assay and show that the addition of fibroblasts to mammary organoid cultures promotes the epithelium to undergo branching morphogenesis, thus allowing the role of the stromal microenvironment to be examined in this essential developmental process.
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- $a Lu, Pengfei $u Department of Anatomy and Program in Developmental and Stem Cell Biology, University of California, San Francisco, CA, 94143-0452, USA. lvpf@shanghaitech.edu.cn. School of Life Science and Technology, Shanghai Tech University, 319 Yueyang Road, Shanghai, 200031, China. lvpf@shanghaitech.edu.cn.
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