Monolithic capillary column with an integrated electrochemical detector
Language English Country Netherlands Media print-electronic
Document type Evaluation Study, Journal Article
PubMed
28647148
DOI
10.1016/j.chroma.2017.06.057
PII: S0021-9673(17)30925-1
Knihovny.cz E-resources
- Keywords
- Dopamine, Electrochemical detection, Integrated column, Polymer monoliths, Urine,
- MeSH
- Dopamine analysis MeSH
- Electrochemical Techniques instrumentation methods MeSH
- Electrodes MeSH
- Humans MeSH
- Neurotransmitter Agents analysis MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Names of Substances
- Dopamine MeSH
- Neurotransmitter Agents MeSH
The carbon fiber and silver microwire were used as working and pseudoreference electrode, respectively, and inserted into the ending of capillary to prepare monolithic capillary column with an integrated electrochemical detector. Prepared capillary devices offered stable and robust results with relative standard deviations of retention, resolution, and detection signal lower than 1.5, 5.5, and 5.0%, respectively. To further increase sensitivity of developed electrochemical microdetector, multiple pulse amperometry detection mode has been used. Optimized integrated device provided reliable chromatographic separation of mixture of neurotransmitters with calibration curve for dopamine linear from 0.5 to 20.0mgL-1 and an instrumental limit of detection as low as 24pg of injected dopamine. Finally, developed capillary column was applied to successful determination of dopamine in a human urine. By using both calibration curve and standard addition method, the dopamine level was determined to be 0.74±0.03mgL-1 and 0.71±0.02mgL-1, respectively. Triplicates of dopamine analysis provided relative standard deviations lower than 2.7% for intraday analyses, while interday relative standard deviations were lower than 3.6% for five consecutive days.
References provided by Crossref.org
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