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Two-photon laser-scanning microscopy for single and repetitive imaging of dorsal and lateral spinal white matter in vivo

F. Nadrigny, K. Le Meur, E. D. Schomburg, S. Safavi-Abbasi, P. Dibaj

. 2017 ; 66 (3) : 531-537. [pub] 20170228

Jazyk angličtina Země Česko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18016052

We developed appropriate surgical procedures for single and repetitive multi-photon imaging of spinal cord in vivo. By intravenous anesthesia, artificial ventilation and laminectomy, acute experiments were performed in the dorsal and lateral white matter. By volatile anesthesia and minimal-invasive surgery, chronic repetitive imaging up to 8 months were performed in the dorsal column through the window between two adjacent spines. Transgenic mouse technology enabled simultaneous imaging of labeled axons, astrocytes and microglia. Repetitive imaging showed positional shifts of microglia over time. These techniques serve for investigations of cellular dynamics and cell-cell interactions in intact and pathologically changed spinal tissue.

Citace poskytuje Crossref.org

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