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Insertion of the voltage-sensitive domain into circularly permuted red fluorescent protein as a design for genetically encoded voltage sensor

LA. Kost, ES. Nikitin, VO. Ivanova, U. Sung, EV. Putintseva, DM. Chudakov, PM. Balaban, KA. Lukyanov, AM. Bogdanov,

. 2017 ; 12 (9) : e0184225. [pub] 20170901

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18016374

Visualization of electrical activity in living cells represents an important challenge in context of basic neurophysiological studies. Here we report a new voltage sensitive fluorescent indicator which response could be detected by fluorescence monitoring in a single red channel. To the best of our knowledge, this is the first fluorescent protein-based voltage sensor which uses insertion-into-circular permutant topology to provide an efficient interaction between sensitive and reporter domains. Its fluorescent core originates from red fluorescent protein (FP) FusionRed, which has optimal spectral characteristics to be used in whole body imaging techniques. Indicators using the same domain topology could become a new perspective for the FP-based voltage sensors that are traditionally based on Förster resonance energy transfer (FRET).

Citace poskytuje Crossref.org

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$a Nikitin, Evgeny S $u Institute of Higher Nervous Activity and Neurophysiology, Moscow, Russian Federation.
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$a Ivanova, Violetta O $u Institute of Higher Nervous Activity and Neurophysiology, Moscow, Russian Federation.
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$a Sung, Uhna $u Center for Functional Connectomics, Korea Institute of Science & Technology, Seoul, Korea.
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$a Putintseva, Ekaterina V $u Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation.
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$a Chudakov, Dmitry M $u Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation. Central European Institute of Technology, Masaryk University, Brno, Czech Republic. Pirogov Russian National Research Medical University, Moscow, Russia.
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$a Balaban, Pavel M $u Institute of Higher Nervous Activity and Neurophysiology, Moscow, Russian Federation.
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$a Lukyanov, Konstantin A $u Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation. Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
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$a Bogdanov, Alexey M $u Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation. Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
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