Visualization of electrical activity in living cells represents an important challenge in context of basic neurophysiological studies. Here we report a new voltage sensitive fluorescent indicator which response could be detected by fluorescence monitoring in a single red channel. To the best of our knowledge, this is the first fluorescent protein-based voltage sensor which uses insertion-into-circular permutant topology to provide an efficient interaction between sensitive and reporter domains. Its fluorescent core originates from red fluorescent protein (FP) FusionRed, which has optimal spectral characteristics to be used in whole body imaging techniques. Indicators using the same domain topology could become a new perspective for the FP-based voltage sensors that are traditionally based on Förster resonance energy transfer (FRET).
- MeSH
- Biosensing Techniques methods MeSH
- Electrophysiological Phenomena MeSH
- Fluorescent Dyes metabolism MeSH
- HEK293 Cells MeSH
- Rats MeSH
- Humans MeSH
- Luminescent Proteins chemistry MeSH
- Cell Line, Tumor MeSH
- Protein Domains MeSH
- Protein Engineering methods MeSH
- Fluorescence Resonance Energy Transfer methods MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
