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Separation of oligopeptides, nucleobases, nucleosides and nucleotides using capillary electrophoresis/electrochromatography with sol-gel modified inner capillary wall
J. Svobodová, O. Kofroňová, O. Benada, V. Král, I. Mikšík,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- chemické techniky analytické přístrojové vybavení metody MeSH
- kapilární elektrochromatografie * MeSH
- mikroskopie elektronová rastrovací MeSH
- nukleosidy izolace a purifikace MeSH
- nukleotidy izolace a purifikace MeSH
- oligopeptidy izolace a purifikace MeSH
- změna skupenství MeSH
- Publikační typ
- časopisecké články MeSH
The aim of this article is to study the modification of an inner capillary wall with sol-gel coating (pure silica sol-gel or silica sol-gel containing porphyrin-brucine conjugate) and determine its influence on the separation process using capillary electrophoresis/electrochromatography method. After modification of the inner capillary surface the separation of analytes was performed using two different phosphate buffers (pH 2.5 and 9.0) and finally the changes in electrophoretic mobilities of various samples were calculated. To confirm that the modification of the inner capillary surface was successful, the parts of the inner surfaces of capillaries were observed using scanning electron microscopy. The analytes used as testing samples were oligopeptides, nucleosides, nucleobases and finally nucleotides.
Department of Analytical Chemistry Institute of Chemical Technology Prague Czech Republic
Institute of Physiology Czech Academy of Sciences Prague Czech Republic
Citace poskytuje Crossref.org
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- $a Svobodová, Jana $u Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic. Electronic address: Jana.Svobodova@fgu.cas.cz.
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- $a The aim of this article is to study the modification of an inner capillary wall with sol-gel coating (pure silica sol-gel or silica sol-gel containing porphyrin-brucine conjugate) and determine its influence on the separation process using capillary electrophoresis/electrochromatography method. After modification of the inner capillary surface the separation of analytes was performed using two different phosphate buffers (pH 2.5 and 9.0) and finally the changes in electrophoretic mobilities of various samples were calculated. To confirm that the modification of the inner capillary surface was successful, the parts of the inner surfaces of capillaries were observed using scanning electron microscopy. The analytes used as testing samples were oligopeptides, nucleosides, nucleobases and finally nucleotides.
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