-
Je něco špatně v tomto záznamu ?
Yeast Tok1p channel is a major contributor to membrane potential maintenance under chemical stress
J. Zahumenský, I. Jančíková, A. Drietomská, A. Švenkrtová, O. Hlaváček, T. Hendrych, J. Plášek, K. Sigler, D. Gášková,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- buněčná membrána MeSH
- draslíkové kanály metabolismus MeSH
- fungální proteiny metabolismus MeSH
- fyziologický stres fyziologie MeSH
- kvasinky metabolismus MeSH
- membránové potenciály fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Tok1p is a highly specific yeast plasma membrane potassium channel with strong outward directionality. Its opening is induced by membrane depolarization. Although the biophysical properties of Tok1p are well-described, its potentially important physiological role is currently largely unexplored. To address this issue, we examined the Tok1p activity following chemically-induced depolarization by measuring changes of plasma membrane potential (ΔΨ) using the diS-C3(3) fluorescence assay in a Tok1p-expressing and a Tok1p-deficient strain. We report that Tok1p channel activity in response to chemical stress does not depend solely on the extent of depolarization, as might have been expected, but may also be negatively influenced by accompanying effects of the used compound. The stressors may interact with the plasma membrane or the channel itself, or cause cytosolic acidification. All of these effects may negatively influence the Tok1p channel opening. While ODDC-induced depolarization exhibits the cleanest Tok1p activation, restoring an astonishing 75% of lost ΔΨ, higher BAC concentrations reduce Tok1p activity, probably because of direct interactions with the channel and/or its lipid microenvironment. This is not only the first study of the physiological role of Tok1p in ΔΨ maintenance under chemical stress, but also the first estimate of the extent of depolarization the channel is able to counterbalance.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18016507
- 003
- CZ-PrNML
- 005
- 20190906111940.0
- 007
- ta
- 008
- 180515s2017 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.bbamem.2017.06.019 $2 doi
- 035 __
- $a (PubMed)28669766
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Zahumenský, Jakub $u Charles University, Faculty of Mathematics and Physics, Institute of Physics, Prague 121 16, Czech Republic.
- 245 10
- $a Yeast Tok1p channel is a major contributor to membrane potential maintenance under chemical stress / $c J. Zahumenský, I. Jančíková, A. Drietomská, A. Švenkrtová, O. Hlaváček, T. Hendrych, J. Plášek, K. Sigler, D. Gášková,
- 520 9_
- $a Tok1p is a highly specific yeast plasma membrane potassium channel with strong outward directionality. Its opening is induced by membrane depolarization. Although the biophysical properties of Tok1p are well-described, its potentially important physiological role is currently largely unexplored. To address this issue, we examined the Tok1p activity following chemically-induced depolarization by measuring changes of plasma membrane potential (ΔΨ) using the diS-C3(3) fluorescence assay in a Tok1p-expressing and a Tok1p-deficient strain. We report that Tok1p channel activity in response to chemical stress does not depend solely on the extent of depolarization, as might have been expected, but may also be negatively influenced by accompanying effects of the used compound. The stressors may interact with the plasma membrane or the channel itself, or cause cytosolic acidification. All of these effects may negatively influence the Tok1p channel opening. While ODDC-induced depolarization exhibits the cleanest Tok1p activation, restoring an astonishing 75% of lost ΔΨ, higher BAC concentrations reduce Tok1p activity, probably because of direct interactions with the channel and/or its lipid microenvironment. This is not only the first study of the physiological role of Tok1p in ΔΨ maintenance under chemical stress, but also the first estimate of the extent of depolarization the channel is able to counterbalance.
- 650 _2
- $a buněčná membrána $7 D002462
- 650 _2
- $a fungální proteiny $x metabolismus $7 D005656
- 650 _2
- $a membránové potenciály $x fyziologie $7 D008564
- 650 _2
- $a draslíkové kanály $x metabolismus $7 D015221
- 650 _2
- $a fyziologický stres $x fyziologie $7 D013312
- 650 _2
- $a kvasinky $x metabolismus $7 D015003
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Jančíková, Iva $u Charles University, Faculty of Mathematics and Physics, Institute of Physics, Prague 121 16, Czech Republic.
- 700 1_
- $a Drietomská, Andrea $u Charles University, Faculty of Mathematics and Physics, Institute of Physics, Prague 121 16, Czech Republic.
- 700 1_
- $a Švenkrtová, Andrea $u Institute of Microbiology, CR Academy of Sciences, Prague 142 20, Czech Republic; Institute of Chemical Technology, Faculty of Food and Biochemical Technology, Prague 166 28, Czech Republic.
- 700 1_
- $a Hlaváček, Otakar $u Institute of Microbiology, CR Academy of Sciences, Prague 142 20, Czech Republic.
- 700 1_
- $a Hendrych, Tomáš $u Department of Genetics and Microbiology, Faculty of Science, Charles University, Prague 128 44, Czech Republic.
- 700 1_
- $a Plášek, Jaromír $u Charles University, Faculty of Mathematics and Physics, Institute of Physics, Prague 121 16, Czech Republic.
- 700 1_
- $a Sigler, Karel $u Institute of Microbiology, CR Academy of Sciences, Prague 142 20, Czech Republic.
- 700 1_
- $a Gášková, Dana $u Charles University, Faculty of Mathematics and Physics, Institute of Physics, Prague 121 16, Czech Republic. Electronic address: gaskova@karlov.mff.cuni.cz.
- 773 0_
- $w MED00000713 $t Biochimica et biophysica acta. Biomembranes $x 0005-2736 $g Roč. 1859, č. 10 (2017), s. 1974-1985
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/28669766 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20180515 $b ABA008
- 991 __
- $a 20190906112314 $b ABA008
- 999 __
- $a ok $b bmc $g 1300131 $s 1013347
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 1859 $c 10 $d 1974-1985 $e 20170629 $i 0005-2736 $m Biochimica et biophysica acta. Biomembranes $n Biochem Biophys Acta $x MED00000713
- LZP __
- $a Pubmed-20180515
