-
Je něco špatně v tomto záznamu ?
Propagation and some physiological effects of Citrus bark cracking viroid and Apple fruit crinkle viroid in multiple infected hop (Humulus lupulus L.)
J. Matoušek, K. Siglová, J. Jakše, S. Radišek, JRJ. Brass, T. Tsushima, T. Guček, GS. Duraisamy, T. Sano, G. Steger,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
- MeSH
- Citrus genetika virologie MeSH
- Humulus genetika virologie MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- Malus genetika virologie MeSH
- messenger RNA genetika MeSH
- ovoce genetika virologie MeSH
- tabák genetika virologie MeSH
- viroidy genetika patogenita MeSH
- Publikační typ
- časopisecké články MeSH
The hop metabolome important for the brewing industry and for medical purposes is endangered worldwide due to multiple viroid infections affecting hop physiology. Combinatorial biolistic hop inoculation with Citrus bark cracking viroid (CBCVd), Apple fruit crinkle viroid (AFCVd), Hop latent viroid, and Hop stunt viroid (HSVd) showed a low CBCVd compatibility with HSVd, while all other viroid combinations were highly compatible. Unlike to other viroids, single CBCVd propagation showed a significant excess of (-) over (+) strands in hop, tomato, and Nicotiana benthamiana, but not in citruses. Inoculation of hop with all viroids led to multiple infections with unstable viroid levels in individual plants in the pre- and post-dormancy periods, and to high plant mortality and morphological disorders. Hop isolates of CBCVd and AFCVd were highly stable, only minor quasispecies were detected. CBCVd caused a strong suppression of some crucial mRNAs related to the hop prenylflavonoid biosynthesis pathway, while AFCVd-caused effects were moderate. According to mRNA degradome analysis, this suppression was not caused by a direct viroid-specific small RNA-mediated degradation. CBCVd infection led to a strong induction of two hop transcription factors from WRKY family and to a disbalance of WRKY/WDR1 complexes important for activation of lupulin genes.
Faculty of Agriculture and Life Science Hirosaki University Bubkyo cho Hirosaki 036 8561 Japan
Institute of Physical Biology Heinrich Heine Universität Düsseldorf D 40204 Düsseldorf Germany
Slovenian Institute of Hop Research and Brewing Cesta Žalskega tabora 2 SI 3310 Žalec Slovenia
University of South Bohemia Faculty of Science Branišovská 31 370 05 České Budějovice Czech Republic
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18016674
- 003
- CZ-PrNML
- 005
- 20180521092754.0
- 007
- ta
- 008
- 180515s2017 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.jplph.2017.02.014 $2 doi
- 035 __
- $a (PubMed)28395198
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Matoušek, J $u Biology Centre ASCR v.v.i, Institute of Plant Molecular Biology, Branišovská 31, České Budějovice 370 05, Czech Republic.
- 245 10
- $a Propagation and some physiological effects of Citrus bark cracking viroid and Apple fruit crinkle viroid in multiple infected hop (Humulus lupulus L.) / $c J. Matoušek, K. Siglová, J. Jakše, S. Radišek, JRJ. Brass, T. Tsushima, T. Guček, GS. Duraisamy, T. Sano, G. Steger,
- 520 9_
- $a The hop metabolome important for the brewing industry and for medical purposes is endangered worldwide due to multiple viroid infections affecting hop physiology. Combinatorial biolistic hop inoculation with Citrus bark cracking viroid (CBCVd), Apple fruit crinkle viroid (AFCVd), Hop latent viroid, and Hop stunt viroid (HSVd) showed a low CBCVd compatibility with HSVd, while all other viroid combinations were highly compatible. Unlike to other viroids, single CBCVd propagation showed a significant excess of (-) over (+) strands in hop, tomato, and Nicotiana benthamiana, but not in citruses. Inoculation of hop with all viroids led to multiple infections with unstable viroid levels in individual plants in the pre- and post-dormancy periods, and to high plant mortality and morphological disorders. Hop isolates of CBCVd and AFCVd were highly stable, only minor quasispecies were detected. CBCVd caused a strong suppression of some crucial mRNAs related to the hop prenylflavonoid biosynthesis pathway, while AFCVd-caused effects were moderate. According to mRNA degradome analysis, this suppression was not caused by a direct viroid-specific small RNA-mediated degradation. CBCVd infection led to a strong induction of two hop transcription factors from WRKY family and to a disbalance of WRKY/WDR1 complexes important for activation of lupulin genes.
- 650 _2
- $a Citrus $x genetika $x virologie $7 D002957
- 650 _2
- $a ovoce $x genetika $x virologie $7 D005638
- 650 _2
- $a Humulus $x genetika $x virologie $7 D027582
- 650 _2
- $a Malus $x genetika $x virologie $7 D027845
- 650 _2
- $a messenger RNA $x genetika $7 D012333
- 650 _2
- $a kvantitativní polymerázová řetězová reakce $7 D060888
- 650 _2
- $a tabák $x genetika $x virologie $7 D014026
- 650 _2
- $a viroidy $x genetika $x patogenita $7 D014772
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Siglová, K $u Biology Centre ASCR v.v.i, Institute of Plant Molecular Biology, Branišovská 31, České Budějovice 370 05, Czech Republic; University of South Bohemia, Faculty of Science, Branišovská 31, 370 05 České Budějovice, Czech Republic.
- 700 1_
- $a Jakše, J $u University of Ljubljana, Biotechnical Faculty, Department of Agronomy, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia.
- 700 1_
- $a Radišek, S $u Slovenian Institute of Hop Research and Brewing, Cesta Žalskega tabora 2, SI-3310 Žalec, Slovenia.
- 700 1_
- $a Brass, Joseph R J $u Institute of Physical Biology, Heinrich-Heine-Universität Düsseldorf, D-40204 Düsseldorf, Germany.
- 700 1_
- $a Tsushima, T $u Faculty of Agriculture and Life Science, Hirosaki University, Bubkyo-cho, Hirosaki 036-8561, Japan.
- 700 1_
- $a Guček, T $u Slovenian Institute of Hop Research and Brewing, Cesta Žalskega tabora 2, SI-3310 Žalec, Slovenia.
- 700 1_
- $a Duraisamy, G S $u Biology Centre ASCR v.v.i, Institute of Plant Molecular Biology, Branišovská 31, České Budějovice 370 05, Czech Republic.
- 700 1_
- $a Sano, T $u Faculty of Agriculture and Life Science, Hirosaki University, Bubkyo-cho, Hirosaki 036-8561, Japan.
- 700 1_
- $a Steger, G $u Institute of Physical Biology, Heinrich-Heine-Universität Düsseldorf, D-40204 Düsseldorf, Germany. Electronic address: steger@biophys.uni-duesseldorf.de.
- 773 0_
- $w MED00008138 $t Journal of plant physiology $x 1618-1328 $g Roč. 213, č. - (2017), s. 166-177
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/28395198 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20180515 $b ABA008
- 991 __
- $a 20180521092935 $b ABA008
- 999 __
- $a ok $b bmc $g 1300298 $s 1013514
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 213 $c - $d 166-177 $e 20170327 $i 1618-1328 $m Journal of plant physiology $n J. plant physiol. $x MED00008138
- LZP __
- $a Pubmed-20180515
