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Anaplasma phagocytophilum Infection Subverts Carbohydrate Metabolic Pathways in the Tick Vector, Ixodes scapularis
A. Cabezas-Cruz, P. Alberdi, JJ. Valdés, M. Villar, J. de la Fuente,
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články, práce podpořená grantem
Free Medical Journals od 2011
PubMed Central od 2011
Europe PubMed Central od 2011
Open Access Digital Library od 2011-01-01
Open Access Digital Library od 2011-01-01
ROAD: Directory of Open Access Scholarly Resources od 2011
Odkazy
PubMed
28229048
DOI
10.3389/fcimb.2017.00023
Knihovny.cz E-zdroje
- MeSH
- Anaplasma phagocytophilum patogenita fyziologie MeSH
- anaplasmóza metabolismus MeSH
- buněčné linie MeSH
- citrátový cyklus genetika MeSH
- glukoneogeneze genetika MeSH
- glykolýza genetika MeSH
- interakce hostitele a patogenu genetika MeSH
- klíště enzymologie genetika metabolismus mikrobiologie MeSH
- metabolické sítě a dráhy genetika MeSH
- metabolismus sacharidů genetika MeSH
- mitochondrie genetika metabolismus MeSH
- pentózofosfátový cyklus genetika MeSH
- proteiny členovců chemie genetika metabolismus MeSH
- proteomika metody MeSH
- regulace genové exprese fyziologie MeSH
- sacharidy MeSH
- slinné žlázy mikrobiologie MeSH
- terciární struktura proteinů MeSH
- transkriptom genetika MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The obligate intracellular pathogen, Anaplasma phagocytophilum, is the causative agent of human, equine, and canine granulocytic anaplasmosis and tick-borne fever (TBF) in ruminants. A. phagocytophilum has become an emerging tick-borne pathogen in the United States, Europe, Africa, and Asia, with increasing numbers of infected people and animals every year. It has been recognized that intracellular pathogens manipulate host cell metabolic pathways to increase infection and transmission in both vertebrate and invertebrate hosts. However, our current knowledge on how A. phagocytophilum affect these processes in the tick vector, Ixodes scapularis is limited. In this study, a genome-wide search for components of major carbohydrate metabolic pathways was performed in I. scapularis ticks for which the genome was recently published. The enzymes involved in the seven major carbohydrate metabolic pathways glycolysis, gluconeogenesis, pentose phosphate, tricarboxylic acid cycle (TCA), glyceroneogenesis, and mitochondrial oxidative phosphorylation and β-oxidation were identified. Then, the available transcriptomics and proteomics data was used to characterize the mRNA and protein levels of I. scapularis major carbohydrate metabolic pathway components in response to A. phagocytophilum infection of tick tissues and cultured cells. The results showed that major carbohydrate metabolic pathways are conserved in ticks. A. phagocytophilum infection inhibits gluconeogenesis and mitochondrial metabolism, but increases the expression of glycolytic genes. A model was proposed to explain how A. phagocytophilum could simultaneously control tick cell glucose metabolism and cytoskeleton organization, which may be achieved in part by up-regulating and stabilizing hypoxia inducible factor 1 alpha in a hypoxia-independent manner. The present work provides a more comprehensive view of the major carbohydrate metabolic pathways involved in the response to A. phagocytophilum infection in ticks, and provides the basis for further studies to develop novel strategies for the control of granulocytic anaplasmosis.
Department of Virology Veterinary Research InstituteBrno Czechia
Faculty of Science University of South BohemiaCeské Budejovice Czechia
Institute of Parasitology Biology Center Czech Academy of SciencesCeské Budejovice Czechia
SaBio Instituto de Investigación en Recursos Cinegéticos Ciudad Real Spain
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