Dasatinib is a novel oral prescription drug proposed for treating adult patients with chronic myeloid leukemia. Three analytical methods, namely ultra high performance liquid chromatography, capillary zone electrophoresis, and sequential injection analysis, were developed, validated, and compared for determination of the drug in the tablet dosage form. The total analysis time of optimized ultra high performance liquid chromatography and capillary zone electrophoresis methods was 2.0 and 2.2 min, respectively. Direct ultraviolet detection with detection wavelength of 322 nm was employed in both cases. The optimized sequential injection analysis method was based on spectrophotometric detection of dasatinib after a simple colorimetric reaction with folin ciocalteau reagent forming a blue-colored complex with an absorbance maximum at 745 nm. The total analysis time was 2.5 min. The ultra high performance liquid chromatography method provided the lowest detection and quantitation limits and the most precise and accurate results. All three newly developed methods were demonstrated to be specific, linear, sensitive, precise, and accurate, providing results satisfactorily meeting the requirements of the pharmaceutical industry, and can be employed for the routine determination of the active pharmaceutical ingredient in the tablet dosage form.

Faculty of Science Department of Analytical Chemistry Charles University Czech Republic

GlaxoSmithKline Currabinny Carrigaline Co Cork Ireland Faculty of Science Department of Analytical Chemistry Charles University Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc18016935

003      

CZ-PrNML

005      

20180515103358.0

007      

ta

008      

180515s2017 gw f 000 0|eng||

009      

AR

024    7_

$a 10.1002/jssc.201600950 $2 doi

035    __

$a (PubMed)27805766

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a gw

100    1_

$a Gonzalez, Aroa Garcia $u GlaxoSmithKline, Currabinny, Carrigaline, Co. Cork, Ireland. Faculty of Science, Department of Analytical Chemistry, Charles University, Czech Republic.

245    10

$a Determination of dasatinib in the tablet dosage form by ultra high performance liquid chromatography, capillary zone electrophoresis, and sequential injection analysis / $c AG. Gonzalez, L. Taraba, J. Hraníček, P. Kozlík, P. Coufal,

520    9_

$a Dasatinib is a novel oral prescription drug proposed for treating adult patients with chronic myeloid leukemia. Three analytical methods, namely ultra high performance liquid chromatography, capillary zone electrophoresis, and sequential injection analysis, were developed, validated, and compared for determination of the drug in the tablet dosage form. The total analysis time of optimized ultra high performance liquid chromatography and capillary zone electrophoresis methods was 2.0 and 2.2 min, respectively. Direct ultraviolet detection with detection wavelength of 322 nm was employed in both cases. The optimized sequential injection analysis method was based on spectrophotometric detection of dasatinib after a simple colorimetric reaction with folin ciocalteau reagent forming a blue-colored complex with an absorbance maximum at 745 nm. The total analysis time was 2.5 min. The ultra high performance liquid chromatography method provided the lowest detection and quantitation limits and the most precise and accurate results. All three newly developed methods were demonstrated to be specific, linear, sensitive, precise, and accurate, providing results satisfactorily meeting the requirements of the pharmaceutical industry, and can be employed for the routine determination of the active pharmaceutical ingredient in the tablet dosage form.

650    _2

$a chemie farmaceutická $x metody $x normy $7 D002626

650    12

$a vysokoúčinná kapalinová chromatografie $7 D002851

650    _2

$a dasatinib $x analýza $7 D000069439

650    12

$a elektroforéza kapilární $7 D019075

650    _2

$a lidé $7 D006801

650    _2

$a limita detekce $7 D057230

650    _2

$a reprodukovatelnost výsledků $7 D015203

650    12

$a spektrofotometrie $7 D013053

650    _2

$a tablety $x chemie $x normy $7 D013607

655    _2

$a časopisecké články $7 D016428

655    _2

$a práce podpořená grantem $7 D013485

700    1_

$a Taraba, Lukáš $u Faculty of Science, Department of Analytical Chemistry, Charles University, Czech Republic.

700    1_

$a Hraníček, Jakub $u Faculty of Science, Department of Analytical Chemistry, Charles University, Czech Republic.

700    1_

$a Kozlík, Petr $u Faculty of Science, Department of Analytical Chemistry, Charles University, Czech Republic.

700    1_

$a Coufal, Pavel $u Faculty of Science, Department of Analytical Chemistry, Charles University, Czech Republic.

773    0_

$w MED00006463 $t Journal of separation science $x 1615-9314 $g Roč. 40, č. 2 (2017), s. 400-406

856    41

$u https://pubmed.ncbi.nlm.nih.gov/27805766 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20180515 $b ABA008

991    __

$a 20180515103531 $b ABA008

999    __

$a ok $b bmc $g 1300559 $s 1013775

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2017 $b 40 $c 2 $d 400-406 $e 20161128 $i 1615-9314 $m Journal of separation science $n J Sep Sci $x MED00006463

LZP    __

$a Pubmed-20180515