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Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes
NP. Jobe, V. Živicová, A. Mifková, D. Rösel, B. Dvořánková, O. Kodet, H. Strnad, M. Kolář, A. Šedo, K. Smetana, K. Strnadová, J. Brábek, L. Lacina,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
Grantová podpora
NV16-29032A
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
NLK
Medline Complete (EBSCOhost)
od 2000-01-01 do Před 1 rokem
- MeSH
- fibroblasty cytologie patologie MeSH
- imunohistochemie MeSH
- invazivní růst nádoru * MeSH
- keratinocyty patologie účinky záření MeSH
- kokultivační techniky MeSH
- kultivované buňky MeSH
- lidé MeSH
- melanom patologie MeSH
- ultrafialové záření * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Melanoma represents a malignant disease with steadily increasing incidence. UV-irradiation is a recognized key factor in melanoma initiation. Therefore, the efficient prevention of UV tissue damage bears a critical potential for melanoma prevention. In this study, we tested the effect of UV irradiation of normal keratinocytes and their consequent interaction with normal and cancer-associated fibroblasts isolated from melanoma, respectively. Using this model of UV influenced microenvironment, we measured melanoma cell migration in 3-D collagen gels. These interactions were studied using DNA microarray technology, immunofluorescence staining, single cell electrophoresis assay, viability (dead/life) cell detection methods, and migration analysis. We observed that three 10 mJ/cm2 fractions at equal intervals over 72 h applied on keratinocytes lead to a 50% increase (p < 0.05) in in vitro invasion of melanoma cells. The introduction cancer-associated fibroblasts to such model further significantly stimulated melanoma cells in vitro invasiveness to a higher extent than normal fibroblasts. A panel of candidate gene products responsible for facilitation of melanoma cells invasion was defined with emphasis on IL-6, IL-8, and CXCL-1. In conclusion, this study demonstrates a synergistic effect between cancer microenvironment and UV irradiation in melanoma invasiveness under in vitro condition.
Citace poskytuje Crossref.org
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- $a Jobe, Njainday Pulo $u Department of Cell Biology, Faculty of Sciences, Charles University in Prague, Viničná 7, 120 00, Prague 2, Czech Republic. Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University in Vestec (BIOCEV), Průmyslová 595, Vestec u Prahy, Prague, Czech Republic. Cell and Experimental Pathology, Department of Translational Medicine, Lund University, Clinical Research Centre, Skåne University Hospital, Jan Waldenströms gata 35, 21421, Malmö, Sweden.
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- $a Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes / $c NP. Jobe, V. Živicová, A. Mifková, D. Rösel, B. Dvořánková, O. Kodet, H. Strnad, M. Kolář, A. Šedo, K. Smetana, K. Strnadová, J. Brábek, L. Lacina,
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- $a Melanoma represents a malignant disease with steadily increasing incidence. UV-irradiation is a recognized key factor in melanoma initiation. Therefore, the efficient prevention of UV tissue damage bears a critical potential for melanoma prevention. In this study, we tested the effect of UV irradiation of normal keratinocytes and their consequent interaction with normal and cancer-associated fibroblasts isolated from melanoma, respectively. Using this model of UV influenced microenvironment, we measured melanoma cell migration in 3-D collagen gels. These interactions were studied using DNA microarray technology, immunofluorescence staining, single cell electrophoresis assay, viability (dead/life) cell detection methods, and migration analysis. We observed that three 10 mJ/cm2 fractions at equal intervals over 72 h applied on keratinocytes lead to a 50% increase (p < 0.05) in in vitro invasion of melanoma cells. The introduction cancer-associated fibroblasts to such model further significantly stimulated melanoma cells in vitro invasiveness to a higher extent than normal fibroblasts. A panel of candidate gene products responsible for facilitation of melanoma cells invasion was defined with emphasis on IL-6, IL-8, and CXCL-1. In conclusion, this study demonstrates a synergistic effect between cancer microenvironment and UV irradiation in melanoma invasiveness under in vitro condition.
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- $a Živicová, Veronika $u Institute of Anatomy, 1st Faculty of Medicine, Charles University, U Nemocnice 3, Prague 2, Czech Republic. Department of Otorhinolaryngology, Head and Neck Surgery, 1st Faculty of Medicine, Charles University, V Úvalu 5, Prague 5, Czech Republic.
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